Strain Name:
C57BL/6J-MtgxR6322Btlr/Mmmh
Stock Number:
044476-MU
Citation ID:
RRID:MMRRC_044476-MU
Other Names:
R6322 (G1)
Major Collection:

Strain Information

Hfe
Name: homeostatic iron regulator
Synonyms: MR2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 15216
HGNC: HGNC:4886
Homologene: 88330
Aldh1l1
Name: aldehyde dehydrogenase 1 family, member L1
Synonyms: 1810048F20Rik, Fthfd
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 107747
HGNC: HGNC:3978
Homologene: 122031
Prkcd
Name: protein kinase C, delta
Synonyms: PKCdelta, PKC[d], Pkcd, D14Ertd420e
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18753
HGNC: HGNC:9399
Homologene: 55963
Dlg1
Name: discs large MAGUK scaffold protein 1
Synonyms: Dlgh1, SAP97, B130052P05Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 13383
HGNC: HGNC:2900
Homologene: 20869
Tns3
Name: tensin 3
Synonyms: TEM6, F830010I22Rik, Tens1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 319939
Homologene: 49713
Mrpl58
Name: mitochondrial ribosomal protein L58
Synonyms: 1110001A02Rik, Ict1, 1110002E03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 68572
HGNC: HGNC:5359
Homologene: 1185
Alox12b
Name: arachidonate 12-lipoxygenase, 12R type
Synonyms: e-LOX2, Aloxe2, 12R-LOX
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 11686
HGNC: HGNC:430
Homologene: 884
Numa1
Name: nuclear mitotic apparatus protein 1
Synonyms: NuMA, 6720401E04Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101706
HGNC: HGNC:8059
Homologene: 38150
Rnmt
Name: RNA (guanine-7-) methyltransferase
Synonyms: 2610002P10Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 67897
Homologene: 2816
Pds5a
Name: PDS5 cohesin associated factor A
Synonyms: 9030416H16Rik, E230024D05Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 71521
Homologene: 22877
Zbtb17
Name: zinc finger and BTB domain containing 17
Synonyms: Miz1, Zfp100, mZ13
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 22642
Homologene: 2575
Sfxn1
Name: sideroflexin 1
Synonyms: 2810002O05Rik, A930015P12Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 14057
VEGA: 13
Homologene: 41494
Map3k20
Name: mitogen-activated protein kinase kinase kinase 20
Synonyms: Zak, MLTKbeta, B230120H23Rik, MLTKalpha
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 65964
Homologene: 32331
Atcay
Name: ataxia, cerebellar, Cayman type
Synonyms: 3322401A10Rik, BNIP-H, ji
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16467
HGNC: HGNC:779
Homologene: 13206
Eif2ak1
Name: eukaryotic translation initiation factor 2 alpha kinase 1
Synonyms: Hri
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 15467
Homologene: 8290
Ubr3
Name: ubiquitin protein ligase E3 component n-recognin 3
Synonyms: A130030D10Rik, Zfp650, 4833421P10Rik, 1110059H15Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 68795
Homologene: 52092
Fchsd1
Name: FCH and double SH3 domains 1
Synonyms: A030002D08Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 319262
Homologene: 14127
Snapc5
Name: small nuclear RNA activating complex, polypeptide 5
Synonyms: 2010103A03Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 330959
VEGA: 9
Homologene: 4414
Fam13c
Name: family with sequence similarity 13, member C
Synonyms: C030038O19Rik, 1200015N20Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 71721
Homologene: 11490
Phf21b
Name: PHD finger protein 21B
Synonyms: A730032D07Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 271305
Homologene: 78154
Plxna2
Name: plexin A2
Synonyms: OCT, Plxn2, 2810428A13Rik, PlexA2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18845
HGNC: HGNC:9100
Homologene: 56427
Moxd1
Name: monooxygenase, DBH-like 1
Synonyms: 3230402N08Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 59012
Homologene: 22904
Slc9a2
Name: solute carrier family 9 (sodium/hydrogen exchanger), member 2
Synonyms: NHE2, 4932415O19Rik, 2210416H12Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226999
Homologene: 20661
Rtn3
Name: reticulon 3
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20168
VEGA: 19
Homologene: 24934
Lama2
Name: laminin, alpha 2
Synonyms: merosin, mer
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16773
HGNC: HGNC:6482
Homologene: 37306
Trappc11
Name: trafficking protein particle complex 11
Synonyms: D030016E14Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 320714
Homologene: 11076
Celsr2
Name: cadherin, EGF LAG seven-pass G-type receptor 2
Synonyms: EGFL2, mfmi1, Adgrc2, flamingo
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 53883
HGNC: HGNC:3231
Homologene: 1078
Cfap44
Name: cilia and flagella associated protein 44
Synonyms: D16Ertd642e, Wdr52, 6330444M21Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 212517
Homologene: 75085
Lipe
Name: lipase, hormone sensitive
Synonyms: 4933403G17Rik, HSL
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16890
HGNC: HGNC:6621
Homologene: 3912
Pappa
Name: pregnancy-associated plasma protein A
Synonyms: PAPP-A, IGFBP-4ase, 8430414N03Rik, PAG1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18491
HGNC: HGNC:8602
Homologene: 31097
Pik3r5
Name: phosphoinositide-3-kinase regulatory subunit 5
Synonyms: p101, Foap2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 320207
Homologene: 8627
Heatr9
Name: HEAT repeat containing 9
Synonyms: Gm11435
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 629303
Homologene: 51895
Gm16432
Name: predicted gene 16432
Type: Gene
Species: Mouse
Chromosome: 1
Plagl2
Name: pleiomorphic adenoma gene-like 2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 54711
HGNC: HGNC:9047
Homologene: 1994
Simc1
Name: SUMO-interacting motifs containing 1
Synonyms: 4732471D19Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 319719
Homologene: 131217
Fam171a1
Name: family with sequence similarity 171, member A1
Synonyms: 9630050M13Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 269233
Homologene: 19521
Nrm
Name: nurim (nuclear envelope membrane protein)
Synonyms: 2610307M02Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 106582
HGNC: HGNC:8003
Homologene: 38294
Adora3
Name: adenosine A3 receptor
Synonyms: 1700001D09Rik, AA3R, A3AR, Gpcr 2, A3R, ARA3, 4930578J19Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11542
HGNC: HGNC:268
Homologene: 550
Rgl1
Name: ral guanine nucleotide dissociation stimulator,-like 1
Synonyms: Rgl
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19731
Homologene: 9039
Sh3bp1
Name: SH3-domain binding protein 1
Synonyms: 3BP-1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20401
Homologene: 7534
Gm15446
Name: predicted gene 15446
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100642166
4932414N04Rik
Name: RIKEN cDNA 4932414N04 gene
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 75721
Homologene: 138468
Sh2b2
Name: SH2B adaptor protein 2
Synonyms: Aps
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 23921
Homologene: 10309
Gm10549
Name: predicted gene 10549
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 433171
VEGA: 18
Cpa6
Name: carboxypeptidase A6
Synonyms: 9030616D13Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 329093
Homologene: 75130
Tmem161a
Name: transmembrane protein 161A
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234371
Homologene: 9853
Krtap4-22
Name: keratin associated protein 4-22
Synonyms: Gm11595
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 100040276
Homologene: 124486
Blvra
Name: biliverdin reductase A
Synonyms: 2500001N03Rik, 0610006A11Rik, Blvr
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 109778
HGNC: HGNC:1062
Homologene: 572
Nkx1-1
Name: NK1 homeobox 1
Synonyms: Nkx-1.1, Sax2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 672284
Homologene: 78303
Myl10
Name: myosin, light chain 10, regulatory
Synonyms: PLRLC-C, 1700027I08Rik, Mylc2pl, PLRLC, PLRLC-A, PLRLC-B
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 59310
Homologene: 69341
Or4f4-ps1
Name: olfactory receptor family 4 subfamily F member 4, pseudogene 1
Synonyms: MOR245-32_p, GA_x6K02T2Q125-72550790-72551728, OTTMUSG00000015084, Olfr1291-ps1, Olfr1291
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 257819
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 10,477,121 bp
  • C to A, chromosome 1 at 40,742,653 bp
  • T to C, chromosome 1 at 152,552,435 bp
  • T to A, chromosome 1 at 178,017,730 bp
  • A to G, chromosome 1 at 194,754,367 bp
  • G to A, chromosome 2 at 3,226,355 bp
  • T to C, chromosome 2 at 68,729,499 bp
  • C to T, chromosome 2 at 69,956,085 bp
  • T to G, chromosome 2 at 72,433,470 bp
  • T to C, chromosome 2 at 111,499,384 bp
  • A to G, chromosome 2 at 127,080,539 bp
  • T to C, chromosome 2 at 153,231,886 bp
  • G to A, chromosome 3 at 105,907,444 bp
  • A to G, chromosome 3 at 108,412,574 bp
  • C to A, chromosome 4 at 65,314,659 bp
  • G to A, chromosome 4 at 141,463,383 bp
  • A to G, chromosome 4 at 144,392,905 bp
  • T to C, chromosome 5 at 33,431,045 bp
  • T to C, chromosome 5 at 65,696,834 bp
  • A to T, chromosome 5 at 109,943,517 bp
  • A to G, chromosome 5 at 136,224,188 bp
  • G to C, chromosome 5 at 136,697,971 bp
  • A to G, chromosome 5 at 143,899,095 bp
  • T to C, chromosome 6 at 90,562,698 bp
  • A to T, chromosome 7 at 25,380,536 bp
  • T to C, chromosome 7 at 102,000,920 bp
  • A to T, chromosome 8 at 47,530,773 bp
  • T to C, chromosome 8 at 70,182,114 bp
  • T to A, chromosome 9 at 64,182,173 bp
  • C to T, chromosome 10 at 24,284,811 bp
  • T to C, chromosome 10 at 27,190,547 bp
  • A to T, chromosome 10 at 70,498,891 bp
  • A to T, chromosome 10 at 81,213,291 bp
  • A to G, chromosome 11 at 8,492,147 bp
  • T to C, chromosome 11 at 68,492,741 bp
  • T to C, chromosome 11 at 69,158,373 bp
  • T to G, chromosome 11 at 83,516,712 bp
  • G to A, chromosome 11 at 99,772,555 bp
  • C to T, chromosome 11 at 115,410,666 bp
  • T to A, chromosome 13 at 23,705,896 bp
  • T to C, chromosome 13 at 54,104,850 bp
  • T to C, chromosome 13 at 54,550,569 bp
  • C to A, chromosome 14 at 30,599,663 bp
  • C to T, chromosome 15 at 78,911,515 bp
  • C to A, chromosome 15 at 84,787,379 bp
  • A to G, chromosome 16 at 31,856,479 bp
  • C to T, chromosome 16 at 44,433,666 bp
  • A to T, chromosome 17 at 35,864,713 bp
  • C to A, chromosome 18 at 33,464,305 bp
  • A to G, chromosome 18 at 37,965,700 bp
  • C to T, chromosome 18 at 68,319,214 bp
  • G to A, chromosome 19 at 7,458,138 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6322 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044476-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.