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Strain Name:
C57BL/6J-MtgxR6341Btlr/Mmmh
Stock Number:
044495-MU
Citation ID:
RRID:MMRRC_044495-MU
Other Names:
R6341 (G1)
Major Collection:

Strain Information

Mafb
Name: MAF bZIP transcription factor B
Synonyms: Kreisler, Krml1, Krml
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16658
HGNC: HGNC:6408
Homologene: 31315
Pkd1
Name: polycystin 1, transient receptor potential channel interacting
Synonyms: polycystin-1, PC1, PC-1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18763
VEGA: 17
HGNC: HGNC:9008
Homologene: 250
Hlcs
Name: holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase)
Synonyms: 410I21.SP6, D16Jhu34
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 110948
VEGA: 16
HGNC: HGNC:4976
Homologene: 37302
Gli2
Name: GLI-Kruppel family member GLI2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14633
HGNC: HGNC:4318
Homologene: 12725
Phax
Name: phosphorylated adaptor for RNA export
Synonyms: p55, 4933427L19Rik, 2810055C14Rik, D18Ertd65e, phosphorylation regulated, Phax, Rnuxa
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 56698
Homologene: 10558
Srgap2
Name: SLIT-ROBO Rho GTPase activating protein 2
Synonyms: FBP2, 9930124L22Rik, Fnbp2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14270
Homologene: 52683
Ddx10
Name: DEAD box helicase 10
Synonyms: 4632415A01Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 10
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 77591
VEGA: 9
HGNC: HGNC:2735
Homologene: 20922
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 3,670,778 bp
  • T to G, chromosome 1 at 23,369,863 bp
  • T to C, chromosome 1 at 39,300,687 bp
  • T to C, chromosome 1 at 45,802,131 bp
  • A to G, chromosome 1 at 87,150,471 bp
  • T to C, chromosome 1 at 118,836,224 bp
  • C to T, chromosome 1 at 131,291,629 bp
  • C to A, chromosome 1 at 191,613,127 bp
  • G to A, chromosome 2 at 52,209,474 bp
  • A to G, chromosome 2 at 83,661,223 bp
  • A to T, chromosome 2 at 90,458,349 bp
  • A to T, chromosome 2 at 114,196,759 bp
  • T to C, chromosome 2 at 122,337,721 bp
  • T to C, chromosome 2 at 152,946,284 bp
  • T to C, chromosome 2 at 160,366,451 bp
  • T to A, chromosome 2 at 178,471,573 bp
  • T to A, chromosome 3 at 97,694,911 bp
  • T to A, chromosome 4 at 40,222,199 bp
  • G to A, chromosome 4 at 121,149,360 bp
  • T to C, chromosome 5 at 67,682,927 bp
  • T to C, chromosome 5 at 74,198,376 bp
  • T to C, chromosome 5 at 92,348,213 bp
  • A to T, chromosome 5 at 129,601,955 bp
  • A to T, chromosome 6 at 42,597,259 bp
  • A to T, chromosome 6 at 58,472,010 bp
  • T to C, chromosome 6 at 87,088,145 bp
  • A to G, chromosome 6 at 119,777,998 bp
  • A to T, chromosome 6 at 119,948,585 bp
  • T to A, chromosome 7 at 4,936,665 bp
  • T to A, chromosome 7 at 23,929,066 bp
  • C to T, chromosome 7 at 35,490,048 bp
  • G to T, chromosome 7 at 35,627,403 bp
  • C to T, chromosome 7 at 45,541,148 bp
  • A to T, chromosome 7 at 141,801,492 bp
  • A to G, chromosome 8 at 45,770,578 bp
  • C to T, chromosome 8 at 71,600,077 bp
  • T to C, chromosome 8 at 84,674,791 bp
  • A to T, chromosome 8 at 85,095,399 bp
  • G to T, chromosome 8 at 95,365,463 bp
  • G to T, chromosome 8 at 124,031,125 bp
  • A to T, chromosome 9 at 36,751,296 bp
  • A to T, chromosome 9 at 53,204,251 bp
  • A to G, chromosome 9 at 54,908,780 bp
  • A to T, chromosome 10 at 75,658,739 bp
  • A to G, chromosome 10 at 81,720,123 bp
  • A to G, chromosome 11 at 23,381,353 bp
  • A to T, chromosome 11 at 51,717,776 bp
  • T to C, chromosome 11 at 67,082,996 bp
  • T to A, chromosome 11 at 68,972,712 bp
  • A to T, chromosome 11 at 95,055,851 bp
  • T to C, chromosome 11 at 106,785,542 bp
  • A to G, chromosome 11 at 109,428,827 bp
  • T to A, chromosome 12 at 61,843,582 bp
  • A to G, chromosome 13 at 91,800,697 bp
  • T to C, chromosome 14 at 89,896,708 bp
  • A to G, chromosome 15 at 10,492,821 bp
  • T to G, chromosome 15 at 44,229,298 bp
  • T to C, chromosome 15 at 76,854,137 bp
  • T to G, chromosome 15 at 83,935,938 bp
  • G to T, chromosome 16 at 5,013,377 bp
  • T to C, chromosome 16 at 16,879,071 bp
  • T to C, chromosome 16 at 17,648,114 bp
  • T to C, chromosome 16 at 31,105,546 bp
  • A to G, chromosome 16 at 94,231,163 bp
  • T to A, chromosome 17 at 24,580,227 bp
  • T to A, chromosome 17 at 26,213,693 bp
  • A to G, chromosome 18 at 37,944,164 bp
  • C to T, chromosome 18 at 56,573,101 bp
  • A to T, chromosome 19 at 4,102,829 bp
  • T to C, chromosome 19 at 12,919,479 bp
  • A to C, chromosome 19 at 39,712,489 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6341 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044495-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.