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Strain Name:
C57BL/6J-MtgxR6481Btlr/Mmmh
Stock Number:
044613-MU
Citation ID:
RRID:MMRRC_044613-MU
Other Names:
R6481 (G1)
Major Collection:

Strain Information

Trmt9b
Name: tRNA methyltransferase 9B
Synonyms: 6430573F11Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 319582
Homologene: 35306
Vsx2
Name: visual system homeobox 2
Synonyms: Hox-10, Hox10, Chx10
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 12677
HGNC: HGNC:1975
Homologene: 7266
Dpagt1
Name: dolichyl-phosphate N-acetylglucosaminephosphotransferase 1
Synonyms: GPT, Dpagt2, Gnpta
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 13478
VEGA: 9
HGNC: HGNC:2995
Homologene: 1058
Slc17a5
Name: solute carrier family 17 (anion/sugar transporter), member 5
Synonyms: 4631416G20Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235504
Homologene: 56571
Cadm1
Name: cell adhesion molecule 1
Synonyms: 3100001I08Rik, 2900073G06Rik, RA175N, RA175C, RA175B, RA175A, Necl2, SgIGSF, Tslc1, SynCam, Igsf4, Igsf4a
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 54725
HGNC: HGNC:5951
Homologene: 8641
Plcd3
Name: phospholipase C, delta 3
Synonyms: 2610205J15Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 72469
HGNC: HGNC:9061
Homologene: 14858
Tnpo3
Name: transportin 3
Synonyms: 5730544L10Rik, C430013M08Rik, D6Ertd313e
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 320938
Homologene: 40848
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 31,222,507 bp
  • A to G, chromosome 1 at 60,438,939 bp
  • A to T, chromosome 1 at 82,453,778 bp
  • T to G, chromosome 1 at 90,958,961 bp
  • T to C, chromosome 1 at 158,612,462 bp
  • G to A, chromosome 2 at 35,902,754 bp
  • T to A, chromosome 2 at 36,793,777 bp
  • T to G, chromosome 2 at 76,741,499 bp
  • A to G, chromosome 2 at 82,990,086 bp
  • T to C, chromosome 2 at 87,753,468 bp
  • A to T, chromosome 2 at 88,852,519 bp
  • A to T, chromosome 2 at 110,795,027 bp
  • T to A, chromosome 3 at 9,699,336 bp
  • T to C, chromosome 3 at 19,694,337 bp
  • T to C, chromosome 3 at 59,861,057 bp
  • A to G, chromosome 3 at 101,883,583 bp
  • A to G, chromosome 4 at 43,498,580 bp
  • A to G, chromosome 4 at 85,067,510 bp
  • A to G, chromosome 4 at 105,794,349 bp
  • A to G, chromosome 4 at 118,726,733 bp
  • G to T, chromosome 4 at 139,431,751 bp
  • A to T, chromosome 4 at 155,753,641 bp
  • A to G, chromosome 4 at 156,249,078 bp
  • A to T, chromosome 5 at 86,492,156 bp
  • T to C, chromosome 5 at 122,800,544 bp
  • T to C, chromosome 5 at 124,577,111 bp
  • T to C, chromosome 5 at 136,744,178 bp
  • G to A, chromosome 5 at 150,386,014 bp
  • A to G, chromosome 6 at 4,538,680 bp
  • T to A, chromosome 6 at 24,796,930 bp
  • G to T, chromosome 6 at 29,571,101 bp
  • C to A, chromosome 6 at 39,805,370 bp
  • T to A, chromosome 6 at 52,216,614 bp
  • A to T, chromosome 6 at 142,604,895 bp
  • A to G, chromosome 7 at 4,145,413 bp
  • A to C, chromosome 7 at 18,814,450 bp
  • A to C, chromosome 7 at 25,288,281 bp
  • A to G, chromosome 7 at 36,752,339 bp
  • A to T, chromosome 7 at 45,442,969 bp
  • A to T, chromosome 7 at 80,364,071 bp
  • A to G, chromosome 7 at 98,853,274 bp
  • A to T, chromosome 7 at 102,494,139 bp
  • A to G, chromosome 7 at 119,534,881 bp
  • A to C, chromosome 7 at 126,112,885 bp
  • A to G, chromosome 7 at 130,185,278 bp
  • A to T, chromosome 7 at 131,256,801 bp
  • G to A, chromosome 7 at 140,969,606 bp
  • A to G, chromosome 7 at 141,809,071 bp
  • T to C, chromosome 8 at 36,498,483 bp
  • G to T, chromosome 8 at 86,634,908 bp
  • A to T, chromosome 8 at 105,358,393 bp
  • C to T, chromosome 9 at 18,550,677 bp
  • T to C, chromosome 9 at 44,331,190 bp
  • A to T, chromosome 9 at 47,788,109 bp
  • G to T, chromosome 9 at 57,023,040 bp
  • A to G, chromosome 9 at 78,538,271 bp
  • C to G, chromosome 9 at 108,837,084 bp
  • A to G, chromosome 9 at 122,857,566 bp
  • A to G, chromosome 10 at 110,774,681 bp
  • T to C, chromosome 10 at 121,661,148 bp
  • T to C, chromosome 11 at 103,077,767 bp
  • G to A, chromosome 11 at 110,165,962 bp
  • T to C, chromosome 12 at 51,598,173 bp
  • C to T, chromosome 12 at 84,593,104 bp
  • A to T, chromosome 13 at 67,347,937 bp
  • A to G, chromosome 13 at 100,128,500 bp
  • A to C, chromosome 13 at 100,162,041 bp
  • A to G, chromosome 15 at 3,965,734 bp
  • T to C, chromosome 15 at 58,923,911 bp
  • T to C, chromosome 15 at 76,658,708 bp
  • T to A, chromosome 15 at 102,162,055 bp
  • T to A, chromosome 16 at 34,360,984 bp
  • A to C, chromosome 16 at 87,378,980 bp
  • T to C, chromosome 17 at 5,922,209 bp
  • C to T, chromosome 17 at 25,845,911 bp
  • G to A, chromosome 17 at 28,226,163 bp
  • G to A, chromosome 17 at 30,748,568 bp
  • C to A, chromosome 17 at 34,645,414 bp
  • T to C, chromosome 17 at 36,268,662 bp
  • T to G, chromosome 17 at 37,772,303 bp
  • G to T, chromosome 17 at 81,388,918 bp
  • A to G, chromosome 17 at 88,711,625 bp
  • T to A, chromosome 18 at 39,150,057 bp
  • T to C, chromosome 19 at 40,802,395 bp
  • A to C, chromosome 19 at 44,973,164 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6481 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044613-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.