Strain Name:
C57BL/6J-MtgxR6554Btlr/Mmmh
Stock Number:
044679-MU
Citation ID:
RRID:MMRRC_044679-MU
Other Names:
R6554 (G1)
Major Collection:

Strain Information

Dop1b
Name: DOP1 leucine zipper like protein B
Synonyms: 2610510B01Rik, 0610038M01Rik, Dopey2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 70028
VEGA: 16
HGNC: HGNC:1291
Homologene: 21068
Mtbp
Name: Mdm2, transformed 3T3 cell double minute p53 binding protein
Synonyms: MDM2BP
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105837
HGNC: HGNC:7417
Homologene: 11102
Unk
Name: unkempt family zinc finger
Synonyms: Zc3h5, B230379M23Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217331
Homologene: 18194
Smc4
Name: structural maintenance of chromosomes 4
Synonyms: Smc4l1, 2500002A22Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 70099
Homologene: 4015
Tbc1d8
Name: TBC1 domain family, member 8
Synonyms: BUB2-like protein 1, GRAM domain, HBLP1, AD3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 54610
Homologene: 31421
Dync1h1
Name: dynein cytoplasmic 1 heavy chain 1
Synonyms: 9930018I23Rik, MAP1C, Swl, Dnec1, Loa, Dnchc1, dynein heavy chain, retrograde transport
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 13424
HGNC: HGNC:2961
Homologene: 1053
Tmem161b
Name: transmembrane protein 161B
Synonyms: 2810446P07Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 72745
Homologene: 14519
Gm14322
Name: predicted gene 14322
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 626802
Nfix
Name: nuclear factor I/X
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 18032
HGNC: HGNC:7788
Homologene: 1872
Lrfn4
Name: leucine rich repeat and fibronectin type III domain containing 4
Synonyms: SALM3
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 225875
Homologene: 36415
Dync2h1
Name: dynein cytoplasmic 2 heavy chain 1
Synonyms: Dnchc2, m152Asp, m407Asp, b2b414Clo, D030010H02Rik, D330044F14Rik, 4432416O06Rik, DHC1b, DHC2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 110350
HGNC: HGNC:2962
Homologene: 14468
Eml2
Name: echinoderm microtubule associated protein like 2
Synonyms: 1600029N02Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 72205
Homologene: 8125
Katnip
Name: katanin interacting protein
Synonyms: D430042O09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233865
Homologene: 45841
Flt3
Name: FMS-like tyrosine kinase 3
Synonyms: wmfl, Flk-2, Flk2, Flt-3, CD135
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14255
HGNC: HGNC:3765
Homologene: 3040
Tbccd1
Name: TBCC domain containing 1
Synonyms: 5730478M09Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 70573
VEGA: 16
Homologene: 32392
Reln
Name: reelin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19699
HGNC: HGNC:9957
Homologene: 3699
Vmn2r61
Name: vomeronasal 2, receptor 61
Synonyms: Gprc2a-rs2, EG637873, Casr-rs2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 637873
Homologene: 129683
A2m
Name: alpha-2-macroglobulin
Synonyms: A2mp
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232345
HGNC: HGNC:7
Homologene: 37248
Klkb1
Name: kallikrein B, plasma 1
Synonyms: Kal-3, PSA, Kal3, Klk3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 16621
HGNC: HGNC:6371
Homologene: 68097
St6gal1
Name: beta galactoside alpha 2,6 sialyltransferase 1
Synonyms: Siat1, ST6Gal I, St6Gal-I
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 20440
Homologene: 2281
Kcnj16
Name: potassium inwardly-rectifying channel, subfamily J, member 16
Synonyms: 6430410F18Rik, Kir5.1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16517
HGNC: HGNC:6262
Homologene: 23112
Cps1
Name: carbamoyl-phosphate synthetase 1
Synonyms: CPS, 4732433M03Rik, CPSase I, D1Ucla3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227231
HGNC: HGNC:2323
Homologene: 68208
Serpina1d
Name: serine (or cysteine) peptidase inhibitor, clade A, member 1D
Synonyms: Spi1-4, PI4
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 20703
HGNC: HGNC:8941
Homologene: 20103
Nsd3
Name: nuclear receptor binding SET domain protein 3
Synonyms: WHISTLE, Whsc1l1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234135
Homologene: 56960
Dmrt2
Name: doublesex and mab-3 related transcription factor 2
Synonyms: Terra
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 226049
VEGA: 19
HGNC: HGNC:2935
Homologene: 17111
Spdl1
Name: spindle apparatus coiled-coil protein 1
Synonyms: 1700018I02Rik, Ccdc99, 2600001J17Rik, 2810049B11Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 70385
Homologene: 9837
Skint8
Name: selection and upkeep of intraepithelial T cells 8
Synonyms: OTTMUSG00000009475
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 639774
Homologene: 106613
Skic8
Name: SKI8 subunit of superkiller complex
Synonyms: 2700038L12Rik, 2810418I05Rik, Wdr61
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 66317
Homologene: 5884
Selplg
Name: selectin, platelet (p-selectin) ligand
Synonyms: CD162, Psgl1, Psgl-1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20345
Homologene: 2261
Ces1b
Name: carboxylesterase 1B
Synonyms: Gm5158
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 382044
HGNC: HGNC:1863
Homologene: 117484
Fam184a
Name: family with sequence similarity 184, member A
Synonyms: 4930589M24Rik, 3110012E06Rik, 4930438C08Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 75906
Homologene: 11600
Zeb2
Name: zinc finger E-box binding homeobox 2
Synonyms: 9130203F04Rik, SIP1, Zfhx1b, Zfx1b, D130016B08Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 24136
Homologene: 8868
Or4g17
Name: olfactory receptor family 4 subfamily G member 17
Synonyms: GA_x6K02T2Q125-72430580-72431515, MOR245-13, Olfr1284
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258379
Homologene: 74059
Mfsd4b5
Name: major facilitator superfamily domain containing 4B5
Synonyms: BC021785
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 215928
Homologene: 129849
Sprr1b
Name: small proline-rich protein 1B
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20754
Homologene: 134537
Or5aq7
Name: olfactory receptor family 5 subfamily AQ member 7
Synonyms: Olfr259, GA_x6K02T2N869-1820-882, MOR172-3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258766
Homologene: 106833
B3galt9
Name: beta-1,3-galactosyltransferase 9
Synonyms: Gm34653
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 102637973
Homologene: 131655
Wdr97
Name: WD repeat domain 97
Synonyms: Gm35339
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 102638882
Homologene: 131539
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 39,406,822 bp
  • C to T, chromosome 1 at 67,174,469 bp
  • C to A, chromosome 2 at 34,839,264 bp
  • A to T, chromosome 2 at 44,997,512 bp
  • A to T, chromosome 2 at 87,107,626 bp
  • C to T, chromosome 2 at 111,379,159 bp
  • G to A, chromosome 2 at 177,768,427 bp
  • G to A, chromosome 3 at 69,029,515 bp
  • T to G, chromosome 3 at 92,437,113 bp
  • G to A, chromosome 4 at 111,927,216 bp
  • T to C, chromosome 5 at 21,896,840 bp
  • G to A, chromosome 5 at 113,820,149 bp
  • A to C, chromosome 5 at 147,375,735 bp
  • C to T, chromosome 6 at 121,641,287 bp
  • G to A, chromosome 7 at 19,201,163 bp
  • A to G, chromosome 7 at 42,276,715 bp
  • C to T, chromosome 7 at 125,850,742 bp
  • A to T, chromosome 8 at 25,662,875 bp
  • T to A, chromosome 8 at 45,273,554 bp
  • T to C, chromosome 8 at 84,727,650 bp
  • C to A, chromosome 8 at 93,064,991 bp
  • A to G, chromosome 9 at 7,037,699 bp
  • A to T, chromosome 9 at 54,727,645 bp
  • T to A, chromosome 10 at 39,986,432 bp
  • T to C, chromosome 10 at 53,640,967 bp
  • T to G, chromosome 11 at 34,822,570 bp
  • T to A, chromosome 11 at 111,025,305 bp
  • T to C, chromosome 11 at 116,051,459 bp
  • A to T, chromosome 12 at 103,764,803 bp
  • T to A, chromosome 12 at 110,649,848 bp
  • C to A, chromosome 13 at 84,222,418 bp
  • A to G, chromosome 15 at 55,567,249 bp
  • A to G, chromosome 15 at 76,354,978 bp
  • A to T, chromosome 16 at 22,822,124 bp
  • A to G, chromosome 16 at 23,321,655 bp
  • A to G, chromosome 16 at 93,760,458 bp
  • T to C, chromosome 19 at 4,613,886 bp
  • C to T, chromosome 19 at 25,677,948 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6554 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044679-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.