Strain Name:
C57BL/6J-MtgxR6657Btlr/Mmmh
Stock Number:
044778-MU
Citation ID:
RRID:MMRRC_044778-MU
Other Names:
R6657 (G1)
Major Collection:

Strain Information

Ep400
Name: E1A binding protein p400
Synonyms: 1700020J09Rik, mDomino, p400
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 75560
Homologene: 38779
Kansl2
Name: KAT8 regulatory NSL complex subunit 2
Synonyms: 2310037I24Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 69612
VEGA: 15
Homologene: 32367
Rictor
Name: RPTOR independent companion of MTOR, complex 2
Synonyms: 4921505C17Rik, 6030405M08Rik, D530039E11Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 78757
VEGA: 15
Homologene: 34317
Chst5
Name: carbohydrate sulfotransferase 5
Synonyms: I-GlcNAc6ST, GST-4
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 56773
Homologene: 56927
Itga5
Name: integrin alpha 5 (fibronectin receptor alpha)
Synonyms: Cd49e, Fnra
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 16402
VEGA: 15
HGNC: HGNC:6141
Homologene: 20508
Washc4
Name: WASH complex subunit 4
Synonyms: A230046K03Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 319277
VEGA: 10
Homologene: 123926
Rsrc2
Name: arginine/serine-rich coiled-coil 2
Synonyms: 1500011J06Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 208606
Homologene: 133892
Vps53
Name: VPS53 GARP complex subunit
Synonyms: 3100002B05Rik, 2010002A08Rik, 2310040I21Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 68299
Homologene: 6264
Sec16a
Name: SEC16 homolog A, endoplasmic reticulum export factor
Synonyms: C230052J16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227648
Homologene: 10533
Sugt1
Name: SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae)
Synonyms: 2410174K12Rik, SGT1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 67955
VEGA: 14
Homologene: 4877
Myh14
Name: myosin, heavy polypeptide 14
Synonyms: 2400004E04Rik, NMHC II-C
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 71960
Homologene: 23480
Zfp592
Name: zinc finger protein 592
Synonyms: A730014M16Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233410
Homologene: 8759
Cpxm2
Name: carboxypeptidase X, M14 family member 2
Synonyms: 4632435C11Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 55987
Homologene: 69259
Nectin2
Name: nectin cell adhesion molecule 2
Synonyms: MPH, Cd112, Pvrl2, nectin-2, Pvs
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 19294
HGNC: HGNC:9707
Homologene: 86092
Csnk1d
Name: casein kinase 1, delta
Synonyms: 1200006A05Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 104318
HGNC: HGNC:2452
Homologene: 74841
Myo19
Name: myosin XIX
Synonyms: Myohd1, 1110055A02Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 66196
Homologene: 49819
Eml5
Name: echinoderm microtubule associated protein like 5
Synonyms: C130068M19Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 319670
VEGA: 12
Homologene: 26807
Zfp599
Name: zinc finger protein 599
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235048
Homologene: 138456
Bhlhe40
Name: basic helix-loop-helix family, member e40
Synonyms: cytokine response gene 8, Clast5, Stra13, Bhlhb2, C130042M06Rik, eip1 (E47 interaction protein 1), Stra14, Sharp2, DEC1, CR8
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 20893
HGNC: HGNC:1046
Homologene: 2722
Mroh7
Name: maestro heat-like repeat family member 7
Synonyms: Gm1027, LOC381538, Heatr8
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 381538
Homologene: 19633
Pcsk2
Name: proprotein convertase subtilisin/kexin type 2
Synonyms: SPC2, prohormone convertase 2, 6330411F23Rik, Nec-2, Phpp-2, Nec2, PC2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18549
HGNC: HGNC:8744
Homologene: 37640
Wdfy4
Name: WD repeat and FYVE domain containing 4
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 545030
Homologene: 83473
Sptbn5
Name: spectrin beta, non-erythrocytic 5
Synonyms: Spnb5, EG640524
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 640524
Homologene: 41150
Odf4
Name: outer dense fiber of sperm tails 4
Synonyms: Oppo1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 252868
Homologene: 17099
Lrp4
Name: low density lipoprotein receptor-related protein 4
Synonyms: 6430526J12Rik, Megf7, mdig
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228357
HGNC: HGNC:6696
Homologene: 17964
Sfmbt1
Name: Scm-like with four mbt domains 1
Synonyms: 4930442N21Rik, Smr, 9330180L21Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 54650
Homologene: 9472
Vmn2r111
Name: vomeronasal 2, receptor 111
Synonyms: EG210876
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 210876
Homologene: 86604
Ttll9
Name: tubulin tyrosine ligase-like family, member 9
Synonyms: 4930509O20Rik, 1700016F23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74711
Homologene: 41769
4933405L10Rik
Name: RIKEN cDNA 4933405L10 gene
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 71046
Homologene: 19274
Fbln2
Name: fibulin 2
Synonyms: 5730577E14Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14115
HGNC: HGNC:3601
Homologene: 1514
Pdzrn3
Name: PDZ domain containing RING finger 3
Synonyms: LNX3, Semcap3, semaphorin cytoplasmic domain-associated protein 3A, 1110020C07Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 55983
Homologene: 10328
Tnfaip6
Name: tumor necrosis factor alpha induced protein 6
Synonyms: Tsg6, TSG-6, Tnfip6
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21930
Homologene: 5162
Sqor
Name: sulfide quinone oxidoreductase
Synonyms: 0610039J17Rik, 4930557M22Rik, flavo-binding protein, Sqrdl
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 59010
Homologene: 10921
Vmn1r173
Name: vomeronasal 1 receptor 173
Synonyms: Gm5892
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 545934
Homologene: 79577
Nrg2
Name: neuregulin 2
Synonyms: NTAK, Don1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 100042150
HGNC: HGNC:7998
Homologene: 75024
Vmn2r52
Name: vomeronasal 2, receptor 52
Synonyms: EG384534
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 384534
Homologene: 129605
Pfpl
Name: pore forming protein-like
Synonyms: Epcs50, Epcs5
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 56093
VEGA: 19
Homologene: 130704
Gpc5
Name: glypican 5
Synonyms: A230034F01Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 103978
HGNC: HGNC:4453
Homologene: 3285
Ctsh
Name: cathepsin H
Synonyms: Cat H
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 13036
HGNC: HGNC:2535
Homologene: 36159
Plbd1
Name: phospholipase B domain containing 1
Synonyms: 1100001H23Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 66857
Homologene: 11745
Akr1b7
Name: aldo-keto reductase family 1, member B7
Synonyms: Avdp, MVDP
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 11997
Homologene: 122176
Hyal6
Name: hyaluronoglucosaminidase 6
Synonyms: 4932701A20Rik, Hyal-ps1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 74409
HGNC: HGNC:5324
Homologene: 78028
Tcp11
Name: t-complex protein 11
Synonyms: Tcp-11, D17Ken1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 21463
Homologene: 8433
Or5aq6
Name: olfactory receptor family 5 subfamily AQ member 6
Synonyms: MOR172-5, GA_x6K02T2Q125-48586461-48585523, Olfr1109
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258762
Homologene: 86672
Akr1b1
Name: aldo-keto reductase family 1 member B
Synonyms: Ahr-1, Akr1b3, Ahr1, Aldr1, ALR2, Aldor1, AR
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 11677
HGNC: HGNC:381
Homologene: 133743
Mmp24
Name: matrix metallopeptidase 24
Synonyms: MT5-MMP, Membrane type 5-MMP
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17391
HGNC: HGNC:7172
Homologene: 21331
Psmb5
Name: proteasome (prosome, macropain) subunit, beta type 5
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 19173
VEGA: 14
HGNC: HGNC:9542
Homologene: 55690
Tmem262
Name: transmembrane protein 262
Synonyms: BC048609
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 433215
Homologene: 104835
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 2 at 26,425,864 bp
  • C to A, chromosome 2 at 52,043,783 bp
  • T to A, chromosome 2 at 87,093,059 bp
  • T to A, chromosome 2 at 91,492,053 bp
  • T to G, chromosome 2 at 120,076,400 bp
  • A to G, chromosome 2 at 122,807,594 bp
  • T to G, chromosome 2 at 143,690,366 bp
  • T to C, chromosome 2 at 152,984,262 bp
  • A to T, chromosome 2 at 155,798,179 bp
  • A to T, chromosome 4 at 106,702,500 bp
  • C to A, chromosome 5 at 110,693,545 bp
  • A to G, chromosome 5 at 123,739,567 bp
  • A to G, chromosome 6 at 24,734,758 bp
  • C to T, chromosome 6 at 34,310,004 bp
  • A to C, chromosome 6 at 34,416,200 bp
  • A to G, chromosome 6 at 91,259,750 bp
  • C to A, chromosome 6 at 101,151,022 bp
  • TG to TGG, chromosome 6 at 108,664,857 bp
  • A to T, chromosome 6 at 136,617,252 bp
  • G to A, chromosome 7 at 10,159,163 bp
  • T to C, chromosome 7 at 19,738,140 bp
  • T to A, chromosome 7 at 23,702,895 bp
  • T to C, chromosome 7 at 44,637,846 bp
  • A to T, chromosome 7 at 81,025,486 bp
  • T to C, chromosome 7 at 132,049,077 bp
  • T to G, chromosome 8 at 105,708,818 bp
  • C to T, chromosome 8 at 111,890,274 bp
  • A to G, chromosome 9 at 22,250,242 bp
  • A to T, chromosome 9 at 90,060,502 bp
  • T to A, chromosome 10 at 83,558,618 bp
  • T to C, chromosome 11 at 68,926,812 bp
  • A to T, chromosome 11 at 76,134,427 bp
  • A to T, chromosome 11 at 84,897,196 bp
  • T to C, chromosome 11 at 120,964,994 bp
  • T to G, chromosome 12 at 98,791,405 bp
  • A to G, chromosome 14 at 30,766,096 bp
  • C to T, chromosome 14 at 33,047,251 bp
  • A to G, chromosome 14 at 54,614,383 bp
  • A to T, chromosome 14 at 79,607,261 bp
  • A to G, chromosome 14 at 115,370,198 bp
  • A to G, chromosome 15 at 6,759,496 bp
  • A to T, chromosome 15 at 76,178,156 bp
  • T to A, chromosome 15 at 98,524,670 bp
  • T to C, chromosome 15 at 103,350,795 bp
  • T to C, chromosome 17 at 22,559,051 bp
  • G to A, chromosome 17 at 28,071,672 bp
  • A to G, chromosome 18 at 36,196,589 bp
  • A to G, chromosome 19 at 6,080,512 bp
  • G to A, chromosome 19 at 12,429,926 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6657 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044778-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.