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Strain Name:
C57BL/6J-MtgxR6838Btlr/Mmmh
Stock Number:
044946-MU
Citation ID:
RRID:MMRRC_044946-MU
Other Names:
R6838 (G1)
Major Collection:

Strain Information

Myo5a
Name: myosin VA
Synonyms: MVa, MyoVA, Myo5, flail, 9630007J19Rik, Dbv
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17918
HGNC: HGNC:7602
Homologene: 20100
Vldlr
Name: very low density lipoprotein receptor
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 22359
Homologene: 443
Gpx1
Name: glutathione peroxidase 1
Synonyms: CGPx, cellular GPx, GSHPx-1, Gpx, GPx-1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 14775
HGNC: HGNC:4553
Homologene: 20155
Tpst1
Name: protein-tyrosine sulfotransferase 1
Synonyms: Tango13a
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22021
Homologene: 2667
Usp28
Name: ubiquitin specific peptidase 28
Synonyms: 9830148O20Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235323
VEGA: 9
Homologene: 10840
Dock9
Name: dedicator of cytokinesis 9
Synonyms: B230309H04Rik, D14Wsu89e, Zizimin1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105445
Homologene: 41026
Map4k4
Name: mitogen-activated protein kinase kinase kinase kinase 4
Synonyms: Nik, 9430080K19Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 26921
HGNC: HGNC:6866
Homologene: 7442
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 39,976,722 bp
  • T to C, chromosome 1 at 46,191,788 bp
  • T to C, chromosome 1 at 74,932,021 bp
  • T to A, chromosome 1 at 131,058,003 bp
  • C to T, chromosome 1 at 136,084,437 bp
  • C to G, chromosome 1 at 173,463,980 bp
  • G to A, chromosome 1 at 185,329,145 bp
  • A to T, chromosome 1 at 194,804,914 bp
  • T to C, chromosome 2 at 115,776,990 bp
  • T to A, chromosome 2 at 150,239,346 bp
  • A to G, chromosome 3 at 88,536,777 bp
  • C to T, chromosome 4 at 48,283,278 bp
  • A to T, chromosome 4 at 138,278,399 bp
  • A to G, chromosome 5 at 38,530,031 bp
  • A to T, chromosome 5 at 76,632,215 bp
  • A to T, chromosome 5 at 76,858,209 bp
  • A to G, chromosome 5 at 91,088,464 bp
  • G to T, chromosome 5 at 107,842,161 bp
  • A to T, chromosome 5 at 130,102,438 bp
  • G to T, chromosome 6 at 12,504,075 bp
  • T to C, chromosome 6 at 108,471,191 bp
  • A to G, chromosome 6 at 118,416,494 bp
  • A to G, chromosome 6 at 143,029,638 bp
  • T to C, chromosome 7 at 4,507,407 bp
  • T to C, chromosome 7 at 15,833,739 bp
  • T to A, chromosome 7 at 22,691,717 bp
  • T to C, chromosome 7 at 31,134,504 bp
  • C to A, chromosome 7 at 44,844,314 bp
  • T to A, chromosome 7 at 56,108,778 bp
  • A to G, chromosome 7 at 88,450,709 bp
  • T to C, chromosome 7 at 102,073,269 bp
  • T to C, chromosome 7 at 127,216,722 bp
  • A to T, chromosome 8 at 70,039,297 bp
  • A to C, chromosome 8 at 80,612,031 bp
  • A to C, chromosome 8 at 88,670,458 bp
  • A to T, chromosome 8 at 105,279,575 bp
  • A to G, chromosome 8 at 106,742,074 bp
  • A to C, chromosome 9 at 37,773,052 bp
  • T to A, chromosome 9 at 49,000,430 bp
  • T to A, chromosome 9 at 71,112,216 bp
  • T to C, chromosome 9 at 75,153,883 bp
  • A to G, chromosome 9 at 108,339,940 bp
  • T to C, chromosome 10 at 62,271,658 bp
  • C to T, chromosome 10 at 108,261,276 bp
  • T to C, chromosome 10 at 119,210,030 bp
  • G to A, chromosome 10 at 127,013,562 bp
  • A to T, chromosome 11 at 73,073,474 bp
  • C to T, chromosome 11 at 73,345,933 bp
  • A to T, chromosome 12 at 87,280,201 bp
  • A to T, chromosome 13 at 21,750,205 bp
  • G to T, chromosome 13 at 55,639,935 bp
  • C to A, chromosome 13 at 84,222,418 bp
  • C to T, chromosome 13 at 99,228,507 bp
  • A to G, chromosome 13 at 99,246,177 bp
  • A to G, chromosome 13 at 102,702,731 bp
  • A to G, chromosome 14 at 31,354,173 bp
  • A to G, chromosome 14 at 121,546,596 bp
  • G to A, chromosome 15 at 85,939,194 bp
  • T to C, chromosome 16 at 26,105,284 bp
  • T to A, chromosome 16 at 90,782,106 bp
  • G to T, chromosome 17 at 28,777,561 bp
  • A to G, chromosome 17 at 30,710,551 bp
  • A to T, chromosome 17 at 37,093,166 bp
  • A to G, chromosome 18 at 46,890,880 bp
  • C to T, chromosome 19 at 4,903,852 bp
  • A to T, chromosome 19 at 8,841,381 bp
  • G to A, chromosome 19 at 27,247,970 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6838 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044946-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.