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Strain Name:
C57BL/6J-MtgxR6917Btlr/Mmmh
Stock Number:
045038-MU
Citation ID:
RRID:MMRRC_045038-MU
Other Names:
R6917 (G1)
Major Collection:

Strain Information

Sp4
Name: trans-acting transcription factor 4
Synonyms: HF1-b, HF-1b, 5730497N03Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 20688
VEGA: 12
Homologene: 2341
Nos2
Name: nitric oxide synthase 2, inducible
Synonyms: iNOS, Nos-2, NOS-II, Nos2a
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18126
HGNC: HGNC:7873
Homologene: 55473
Thrap3
Name: thyroid hormone receptor associated protein 3
Synonyms: 9330151F09Rik, Trap150, B230333E16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230753
Homologene: 31289
Gpatch2l
Name: G patch domain containing 2 like
Synonyms: 1700020O03Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 70373
VEGA: 12
Homologene: 9942
Dmxl1
Name: Dmx-like 1
Synonyms: C630007L23Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 240283
HGNC: HGNC:2937
Homologene: 21136
Slc19a2
Name: solute carrier family 19 (thiamine transporter), member 2
Synonyms: TRMA, THTR1, DDA1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 116914
Homologene: 38258
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 37,995,806 bp
  • C to A, chromosome 1 at 164,261,009 bp
  • C to T, chromosome 2 at 28,892,409 bp
  • A to G, chromosome 2 at 32,631,152 bp
  • ATT to ATTTT, chromosome 2 at 181,338,277 bp
  • C to T, chromosome 3 at 66,994,649 bp
  • T to C, chromosome 4 at 98,734,031 bp
  • A to G, chromosome 4 at 102,968,191 bp
  • A to T, chromosome 4 at 118,809,129 bp
  • A to G, chromosome 4 at 126,180,492 bp
  • A to G, chromosome 6 at 3,996,680 bp
  • T to C, chromosome 6 at 139,896,173 bp
  • T to C, chromosome 7 at 30,958,296 bp
  • T to A, chromosome 7 at 38,522,190 bp
  • CCCTCCTCCTCCTCCTCCTCCT to CCCTCCTCCTCCTCCTCCT, chromosome 7 at 62,377,844 bp
  • A to G, chromosome 7 at 65,299,688 bp
  • T to C, chromosome 7 at 98,095,763 bp
  • T to G, chromosome 7 at 140,110,011 bp
  • T to C, chromosome 8 at 13,172,563 bp
  • T to A, chromosome 9 at 37,541,449 bp
  • T to A, chromosome 9 at 39,511,199 bp
  • T to A, chromosome 9 at 92,593,770 bp
  • A to T, chromosome 9 at 122,862,294 bp
  • A to G, chromosome 10 at 23,865,934 bp
  • A to G, chromosome 11 at 69,406,593 bp
  • A to G, chromosome 11 at 70,661,436 bp
  • C to T, chromosome 11 at 78,951,227 bp
  • C to T, chromosome 11 at 82,879,333 bp
  • A to G, chromosome 12 at 53,069,168 bp
  • A to G, chromosome 12 at 72,184,905 bp
  • G to A, chromosome 12 at 86,244,184 bp
  • G to T, chromosome 12 at 118,299,173 bp
  • A to T, chromosome 13 at 68,620,757 bp
  • A to T, chromosome 13 at 70,594,894 bp
  • G to A, chromosome 14 at 34,555,364 bp
  • A to T, chromosome 14 at 44,858,610 bp
  • A to G, chromosome 14 at 50,643,223 bp
  • A to G, chromosome 14 at 55,652,407 bp
  • A to G, chromosome 14 at 101,918,010 bp
  • A to G, chromosome 16 at 56,617,321 bp
  • A to T, chromosome 16 at 64,786,471 bp
  • T to C, chromosome 17 at 34,283,707 bp
  • T to C, chromosome 17 at 56,309,574 bp
  • T to G, chromosome 17 at 81,044,114 bp
  • A to G, chromosome 18 at 48,047,589 bp
  • T to C, chromosome 18 at 49,864,148 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6917 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045038-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.