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Strain Name:
C57BL/6J-MtgxR6967Btlr/Mmmh
Stock Number:
045077-MU
Citation ID:
RRID:MMRRC_045077-MU
Other Names:
R6967 (G1)
Major Collection:

Strain Information

Gga3
Name: golgi associated, gamma adaptin ear containing, ARF binding protein 3
Synonyms: C230037M19Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 260302
Homologene: 121703
Nub1
Name: negative regulator of ubiquitin-like proteins 1
Synonyms: BS4, NY-REN-18, 4931404D21Rik, 6330412F12Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 53312
Homologene: 41108
Cdc42ep4
Name: CDC42 effector protein 4
Synonyms: CEP4, Borg4, 1500041M20Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 56699
Homologene: 8110
Tmem63b
Name: transmembrane protein 63b
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224807
Homologene: 101682
Ggta1
Name: glycoprotein galactosyltransferase alpha 1, 3
Synonyms: GALT, alpha Gal, Gal, glycoprotein alpha galactosyl transferase 1, alpha3GalT, Ggta-1, Ggta
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14594
HGNC: HGNC:4253
Homologene: 7730
Adam23
Name: a disintegrin and metallopeptidase domain 23
Synonyms: MDC3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 23792
HGNC: HGNC:202
Homologene: 2826
Hcn4
Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 330953
VEGA: 9
Homologene: 3997
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 10,153,678 bp
  • G to T, chromosome 1 at 10,153,679 bp
  • T to G, chromosome 1 at 63,563,336 bp
  • C to T, chromosome 1 at 88,215,123 bp
  • A to T, chromosome 1 at 119,684,581 bp
  • G to A, chromosome 2 at 26,430,486 bp
  • A to G, chromosome 2 at 35,402,722 bp
  • A to G, chromosome 2 at 53,097,332 bp
  • A to G, chromosome 2 at 87,575,513 bp
  • T to C, chromosome 3 at 153,206,708 bp
  • A to G, chromosome 4 at 16,158,275 bp
  • A to T, chromosome 4 at 34,807,812 bp
  • A to G, chromosome 4 at 115,898,294 bp
  • T to C, chromosome 5 at 24,708,711 bp
  • T to C, chromosome 5 at 36,615,756 bp
  • T to C, chromosome 5 at 108,778,914 bp
  • T to C, chromosome 5 at 125,422,167 bp
  • A to G, chromosome 6 at 42,487,013 bp
  • A to C, chromosome 6 at 48,489,794 bp
  • A to T, chromosome 6 at 128,710,523 bp
  • C to T, chromosome 7 at 4,964,147 bp
  • A to T, chromosome 7 at 11,756,617 bp
  • T to C, chromosome 7 at 15,972,205 bp
  • G to GACGGCGGCA, chromosome 7 at 97,579,909 bp
  • A to G, chromosome 7 at 104,059,005 bp
  • T to A, chromosome 7 at 118,547,678 bp
  • A to G, chromosome 7 at 126,870,392 bp
  • T to A, chromosome 8 at 70,060,552 bp
  • G to A, chromosome 8 at 93,267,997 bp
  • T to C, chromosome 8 at 124,688,618 bp
  • C to T, chromosome 9 at 30,954,491 bp
  • T to C, chromosome 9 at 35,034,906 bp
  • T to C, chromosome 9 at 45,674,523 bp
  • G to A, chromosome 9 at 56,890,136 bp
  • C to T, chromosome 9 at 58,823,945 bp
  • C to T, chromosome 9 at 61,972,831 bp
  • A to T, chromosome 10 at 7,839,880 bp
  • T to C, chromosome 10 at 20,988,625 bp
  • G to A, chromosome 10 at 107,814,050 bp
  • T to C, chromosome 11 at 62,847,603 bp
  • C to T, chromosome 11 at 87,889,498 bp
  • T to A, chromosome 11 at 100,134,513 bp
  • C to T, chromosome 11 at 113,729,172 bp
  • T to C, chromosome 11 at 115,591,276 bp
  • A to T, chromosome 13 at 3,574,819 bp
  • ACTTCTTCT to ACTTCT, chromosome 13 at 59,649,576 bp
  • A to T, chromosome 13 at 100,539,288 bp
  • A to T, chromosome 14 at 66,150,949 bp
  • A to G, chromosome 14 at 79,597,407 bp
  • A to T, chromosome 14 at 97,903,197 bp
  • A to C, chromosome 15 at 75,158,549 bp
  • T to C, chromosome 17 at 45,666,632 bp
  • A to C, chromosome 17 at 48,392,904 bp
  • G to T, chromosome 17 at 53,463,168 bp
  • C to A, chromosome 18 at 35,980,332 bp
  • A to T, chromosome 19 at 11,414,827 bp
  • A to G, chromosome 19 at 13,375,451 bp
  • T to A, chromosome 19 at 34,040,394 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6967 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045077-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.