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Strain Name:
C57BL/6J-MtgxR6994Btlr/Mmmh
Stock Number:
045100-MU
Citation ID:
RRID:MMRRC_045100-MU
Other Names:
R6994 (G1)
Major Collection:

Strain Information

Vcan
Name: versican
Synonyms: PG-M, hdf, heart defect, 5430420N07Rik, Cspg2, DPEAAE
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 13003
HGNC: HGNC:2464
Homologene: 3228
Dtnbp1
Name: dystrobrevin binding protein 1
Synonyms: dysbindin, 5430437B18Rik, sdy, Bloc1s8
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 94245
VEGA: 13
Homologene: 12037
Psenen
Name: presenilin enhancer gamma secretase subunit
Synonyms: 1700023M09Rik, Pen2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 66340
Homologene: 11952
Spen
Name: spen family transcription repressor
Synonyms: Mint
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56381
Homologene: 124461
Macroh2a1
Name: macroH2A.1 histone
Synonyms: mH2a1, MACROH2A1.2, H2AF12M, H2afy
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 26914
HGNC: HGNC:4740
Homologene: 3598
Ddx10
Name: DEAD box helicase 10
Synonyms: 4632415A01Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 10
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 77591
VEGA: 9
HGNC: HGNC:2735
Homologene: 20922
Trip10
Name: thyroid hormone receptor interactor 10
Synonyms: Cip4
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 106628
VEGA: 17
Homologene: 99728
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 12,881,393 bp
  • T to C, chromosome 1 at 31,222,909 bp
  • C to A, chromosome 1 at 65,252,530 bp
  • T to A, chromosome 1 at 74,293,176 bp
  • T to A, chromosome 1 at 139,736,930 bp
  • G to A, chromosome 1 at 153,136,762 bp
  • C to T, chromosome 1 at 173,455,586 bp
  • T to C, chromosome 2 at 25,083,309 bp
  • T to C, chromosome 2 at 25,617,045 bp
  • T to A, chromosome 2 at 106,699,533 bp
  • T to C, chromosome 2 at 120,963,593 bp
  • A to T, chromosome 2 at 158,258,244 bp
  • C to T, chromosome 2 at 160,904,883 bp
  • T to G, chromosome 2 at 167,997,266 bp
  • C to T, chromosome 2 at 181,493,668 bp
  • C to A, chromosome 3 at 46,446,910 bp
  • C to T, chromosome 3 at 66,994,649 bp
  • A to G, chromosome 4 at 43,171,403 bp
  • A to G, chromosome 4 at 43,173,203 bp
  • A to G, chromosome 4 at 43,690,192 bp
  • T to C, chromosome 4 at 141,493,459 bp
  • T to G, chromosome 4 at 144,623,279 bp
  • T to A, chromosome 5 at 67,944,669 bp
  • A to G, chromosome 5 at 107,133,026 bp
  • A to T, chromosome 5 at 109,340,103 bp
  • T to C, chromosome 5 at 113,614,922 bp
  • T to C, chromosome 5 at 124,714,145 bp
  • T to C, chromosome 6 at 78,381,149 bp
  • T to A, chromosome 6 at 89,894,158 bp
  • A to T, chromosome 6 at 115,451,050 bp
  • T to C, chromosome 7 at 16,203,874 bp
  • G to T, chromosome 7 at 19,702,906 bp
  • A to G, chromosome 7 at 26,939,181 bp
  • A to C, chromosome 7 at 28,693,360 bp
  • G to A, chromosome 7 at 30,425,767 bp
  • A to G, chromosome 7 at 30,563,507 bp
  • A to T, chromosome 7 at 43,465,067 bp
  • A to C, chromosome 7 at 100,853,114 bp
  • T to C, chromosome 7 at 103,172,912 bp
  • T to A, chromosome 7 at 108,322,894 bp
  • A to G, chromosome 7 at 127,016,441 bp
  • A to T, chromosome 8 at 24,786,246 bp
  • T to C, chromosome 8 at 43,190,078 bp
  • C to T, chromosome 8 at 71,623,521 bp
  • CT to C, chromosome 8 at 104,182,032 bp
  • T to C, chromosome 8 at 123,830,366 bp
  • C to T, chromosome 9 at 21,287,787 bp
  • T to C, chromosome 9 at 44,628,723 bp
  • G to A, chromosome 9 at 50,792,122 bp
  • A to G, chromosome 9 at 53,204,111 bp
  • T to C, chromosome 9 at 75,509,387 bp
  • A to G, chromosome 10 at 45,339,418 bp
  • C to T, chromosome 10 at 130,391,007 bp
  • T to A, chromosome 11 at 100,086,445 bp
  • T to A, chromosome 11 at 101,165,693 bp
  • C to A, chromosome 12 at 54,068,793 bp
  • G to A, chromosome 12 at 86,244,184 bp
  • G to A, chromosome 12 at 104,112,830 bp
  • T to C, chromosome 13 at 34,127,535 bp
  • T to A, chromosome 13 at 45,001,929 bp
  • T to C, chromosome 13 at 56,089,830 bp
  • T to A, chromosome 13 at 61,539,884 bp
  • A to T, chromosome 13 at 89,693,407 bp
  • G to C, chromosome 13 at 112,368,300 bp
  • T to A, chromosome 14 at 51,009,681 bp
  • G to T, chromosome 14 at 52,753,591 bp
  • G to T, chromosome 14 at 53,042,476 bp
  • T to C, chromosome 14 at 72,480,831 bp
  • A to T, chromosome 14 at 78,908,156 bp
  • T to A, chromosome 14 at 79,213,690 bp
  • G to T, chromosome 15 at 99,942,466 bp
  • T to C, chromosome 16 at 14,128,857 bp
  • C to T, chromosome 16 at 35,931,372 bp
  • G to A, chromosome 16 at 48,565,621 bp
  • A to T, chromosome 16 at 48,618,546 bp
  • T to G, chromosome 16 at 59,284,090 bp
  • T to A, chromosome 17 at 50,036,991 bp
  • A to T, chromosome 17 at 52,977,695 bp
  • A to G, chromosome 17 at 57,255,331 bp
  • G to A, chromosome 17 at 67,753,825 bp
  • A to T, chromosome 18 at 38,198,500 bp
  • G to T, chromosome 18 at 80,653,564 bp
  • A to G, chromosome 18 at 89,028,899 bp
  • A to T, chromosome 19 at 5,873,181 bp
  • A to T, chromosome 19 at 6,999,727 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6994 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045100-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.