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Strain Name:
C57BL/6J-MtgxR6996Btlr/Mmmh
Stock Number:
045102-MU
Citation ID:
RRID:MMRRC_045102-MU
Other Names:
R6996 (G1)
Major Collection:

Strain Information

Bcar3
Name: breast cancer anti-estrogen resistance 3
Synonyms: AND-34
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 29815
HGNC: HGNC:973
Homologene: 31181
Efr3a
Name: EFR3 homolog A
Synonyms: A130089M23Rik, D030063F01Rik, C920006C10Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 76740
Homologene: 44222
Spop
Name: speckle-type BTB/POZ protein
Synonyms: TEF2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20747
Homologene: 68354
Pml
Name: promyelocytic leukemia
Synonyms: Trim19, 1200009E24Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 18854
HGNC: HGNC:9113
Homologene: 13245
Specc1l
Name: sperm antigen with calponin homology and coiled-coil domains 1-like
Synonyms: 4930470P14Rik, 9530057A13Rik, 4932439K10Rik, Specc1l, Cytsa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 74392
VEGA: 10
Homologene: 15610
Sap30bp
Name: SAP30 binding protein
Synonyms: 2700016D05Rik, Hcngp
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 57230
Homologene: 8308
Reps1
Name: RalBP1 associated Eps domain containing protein
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19707
Homologene: 7515
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 56,636,410 bp
  • A to T, chromosome 1 at 180,587,371 bp
  • T to C, chromosome 1 at 180,851,189 bp
  • A to C, chromosome 2 at 51,627,589 bp
  • A to T, chromosome 2 at 66,287,731 bp
  • A to G, chromosome 2 at 88,623,845 bp
  • A to G, chromosome 2 at 155,024,437 bp
  • A to G, chromosome 2 at 181,996,204 bp
  • A to C, chromosome 3 at 58,636,095 bp
  • G to A, chromosome 3 at 63,346,102 bp
  • A to T, chromosome 3 at 93,202,670 bp
  • A to C, chromosome 3 at 93,202,949 bp
  • T to C, chromosome 3 at 116,328,706 bp
  • A to G, chromosome 3 at 117,846,632 bp
  • A to G, chromosome 3 at 122,508,384 bp
  • T to C, chromosome 4 at 33,158,157 bp
  • A to G, chromosome 4 at 123,117,687 bp
  • G to T, chromosome 5 at 34,327,260 bp
  • C to A, chromosome 5 at 142,212,014 bp
  • A to G, chromosome 6 at 60,977,383 bp
  • C to A, chromosome 7 at 6,378,765 bp
  • T to C, chromosome 7 at 24,997,626 bp
  • C to G, chromosome 7 at 64,388,921 bp
  • A to G, chromosome 7 at 80,343,750 bp
  • CGGCGGCGG to CGGCGGCGGGGGCGGCGG, chromosome 7 at 97,579,911 bp
  • C to A, chromosome 7 at 99,591,362 bp
  • T to G, chromosome 7 at 103,639,858 bp
  • A to T, chromosome 7 at 104,596,811 bp
  • A to G, chromosome 7 at 133,876,898 bp
  • T to C, chromosome 8 at 4,266,611 bp
  • C to T, chromosome 8 at 10,569,496 bp
  • G to A, chromosome 8 at 24,806,501 bp
  • C to G, chromosome 9 at 7,791,070 bp
  • C to A, chromosome 9 at 18,645,897 bp
  • G to A, chromosome 9 at 36,128,639 bp
  • A to C, chromosome 9 at 42,366,786 bp
  • C to T, chromosome 9 at 56,057,561 bp
  • A to G, chromosome 9 at 58,234,886 bp
  • T to C, chromosome 9 at 75,250,464 bp
  • T to C, chromosome 9 at 110,550,572 bp
  • T to A, chromosome 10 at 18,093,855 bp
  • T to C, chromosome 10 at 40,249,778 bp
  • C to A, chromosome 10 at 75,246,279 bp
  • T to A, chromosome 10 at 77,997,589 bp
  • C to A, chromosome 10 at 78,847,517 bp
  • T to C, chromosome 10 at 127,045,018 bp
  • T to A, chromosome 10 at 129,526,863 bp
  • C to T, chromosome 11 at 8,849,046 bp
  • T to A, chromosome 11 at 88,999,503 bp
  • A to G, chromosome 11 at 95,471,310 bp
  • A to G, chromosome 11 at 100,047,732 bp
  • A to T, chromosome 11 at 103,618,757 bp
  • A to G, chromosome 11 at 115,933,488 bp
  • C to T, chromosome 12 at 16,723,354 bp
  • T to A, chromosome 12 at 76,028,012 bp
  • G to A, chromosome 12 at 86,244,184 bp
  • A to T, chromosome 13 at 59,642,244 bp
  • C to T, chromosome 13 at 70,798,038 bp
  • G to A, chromosome 13 at 97,149,360 bp
  • G to A, chromosome 13 at 100,043,813 bp
  • A to T, chromosome 14 at 31,615,423 bp
  • A to T, chromosome 14 at 70,669,448 bp
  • A to T, chromosome 14 at 122,464,668 bp
  • T to A, chromosome 15 at 65,848,181 bp
  • A to T, chromosome 15 at 79,362,759 bp
  • T to C, chromosome 15 at 99,955,493 bp
  • A to G, chromosome 16 at 16,868,814 bp
  • T to C, chromosome 16 at 17,980,577 bp
  • G to A, chromosome 16 at 38,397,468 bp
  • A to G, chromosome 16 at 59,074,192 bp
  • A to G, chromosome 17 at 18,304,641 bp
  • T to C, chromosome 17 at 47,395,177 bp
  • T to A, chromosome 18 at 37,806,603 bp
  • C to A, chromosome 18 at 46,720,313 bp
  • C to T, chromosome 18 at 61,201,854 bp
  • T to C, chromosome 18 at 80,323,143 bp
  • G to T, chromosome 19 at 4,345,641 bp
  • A to T, chromosome 19 at 13,313,672 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6996 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045102-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.