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Strain Name:
C57BL/6J-MtgxR7033Btlr/Mmmh
Stock Number:
045134-MU
Citation ID:
RRID:MMRRC_045134-MU
Other Names:
R7033 (G1)
Major Collection:

Strain Information

Chrm4
Name: cholinergic receptor, muscarinic 4
Synonyms: Chrm-4, muscarinic acetylcholine receptor 4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12672
HGNC: HGNC:1953
Homologene: 20192
Ank2
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-2, Ankyrin-B, Gm4392
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109676
HGNC: HGNC:493
H2-DMa
Name: histocompatibility 2, class II, locus DMa
Synonyms: H2-Ma, H-2Ma, H2-M alpha
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 14998
HGNC: HGNC:4934
Homologene: 4464
Ubc
Name: ubiquitin C
Synonyms: 2700054O04Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22190
Homologene: 128418
Psen2
Name: presenilin 2
Synonyms: ALG-3, PS-2, PS2, Ad4h
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19165
HGNC: HGNC:9509
Homologene: 386
Slc27a4
Name: solute carrier family 27 (fatty acid transporter), member 4
Synonyms: fatty acid transport protein 4, FATP4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 26569
Homologene: 68437
Serpinc1
Name: serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1
Synonyms: antithrombin, ATIII, At-3, At3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 11905
HGNC: HGNC:775
Homologene: 20139
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 53,479,661 bp
  • G to A, chromosome 1 at 60,310,947 bp
  • A to T, chromosome 1 at 88,095,528 bp
  • A to G, chromosome 1 at 160,997,521 bp
  • C to T, chromosome 1 at 180,227,520 bp
  • T to C, chromosome 1 at 184,037,605 bp
  • G to A, chromosome 2 at 28,229,336 bp
  • T to C, chromosome 2 at 29,804,271 bp
  • T to A, chromosome 2 at 88,700,820 bp
  • A to G, chromosome 2 at 91,928,347 bp
  • T to C, chromosome 2 at 112,256,168 bp
  • T to C, chromosome 2 at 129,115,642 bp
  • T to A, chromosome 2 at 131,953,442 bp
  • T to C, chromosome 2 at 151,557,500 bp
  • T to C, chromosome 2 at 151,970,211 bp
  • A to G, chromosome 3 at 96,169,946 bp
  • C to A, chromosome 3 at 126,944,850 bp
  • A to G, chromosome 4 at 10,898,014 bp
  • A to G, chromosome 4 at 32,639,392 bp
  • A to G, chromosome 4 at 52,826,089 bp
  • C to T, chromosome 4 at 80,911,367 bp
  • T to G, chromosome 4 at 118,613,126 bp
  • G to T, chromosome 5 at 10,950,551 bp
  • T to G, chromosome 5 at 25,692,709 bp
  • C to T, chromosome 5 at 99,232,336 bp
  • T to A, chromosome 5 at 121,364,568 bp
  • T to A, chromosome 5 at 125,388,174 bp
  • G to C, chromosome 5 at 136,697,971 bp
  • A to T, chromosome 6 at 4,516,904 bp
  • A to G, chromosome 6 at 29,171,148 bp
  • A to G, chromosome 6 at 135,923,038 bp
  • T to C, chromosome 6 at 142,472,722 bp
  • T to C, chromosome 7 at 4,338,145 bp
  • T to C, chromosome 7 at 18,614,744 bp
  • C to T, chromosome 7 at 22,842,864 bp
  • T to A, chromosome 7 at 46,267,398 bp
  • A to T, chromosome 7 at 96,895,223 bp
  • A to T, chromosome 8 at 4,349,641 bp
  • T to C, chromosome 8 at 27,031,764 bp
  • G to T, chromosome 8 at 69,765,841 bp
  • G to C, chromosome 8 at 70,138,170 bp
  • C to T, chromosome 9 at 42,030,983 bp
  • G to T, chromosome 9 at 56,259,707 bp
  • T to C, chromosome 9 at 56,888,074 bp
  • T to C, chromosome 10 at 3,940,251 bp
  • A to G, chromosome 10 at 81,462,714 bp
  • A to G, chromosome 10 at 88,754,475 bp
  • A to C, chromosome 10 at 108,690,936 bp
  • C to A, chromosome 11 at 5,798,102 bp
  • T to C, chromosome 11 at 48,915,999 bp
  • C to A, chromosome 11 at 55,223,737 bp
  • A to G, chromosome 11 at 67,369,316 bp
  • A to T, chromosome 11 at 69,747,213 bp
  • C to T, chromosome 11 at 86,233,085 bp
  • G to A, chromosome 12 at 49,384,720 bp
  • A to T, chromosome 12 at 87,960,281 bp
  • A to T, chromosome 12 at 105,176,491 bp
  • T to C, chromosome 12 at 116,211,891 bp
  • T to C, chromosome 13 at 4,079,178 bp
  • A to G, chromosome 14 at 31,264,925 bp
  • A to G, chromosome 14 at 41,997,417 bp
  • A to G, chromosome 14 at 50,736,707 bp
  • A to C, chromosome 15 at 68,181,015 bp
  • G to A, chromosome 15 at 89,371,356 bp
  • T to C, chromosome 15 at 89,569,500 bp
  • C to A, chromosome 16 at 32,756,324 bp
  • G to A, chromosome 16 at 59,554,165 bp
  • T to C, chromosome 17 at 20,208,612 bp
  • A to G, chromosome 17 at 21,500,246 bp
  • A to G, chromosome 17 at 32,199,015 bp
  • A to T, chromosome 17 at 33,883,697 bp
  • T to A, chromosome 17 at 34,136,997 bp
  • A to G, chromosome 17 at 55,866,009 bp
  • A to G, chromosome 18 at 47,248,570 bp
  • G to T, chromosome 18 at 61,307,714 bp
  • A to G, chromosome 19 at 9,893,372 bp
  • A to G, chromosome 19 at 42,124,977 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7033 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045134-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.