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Strain Name:
C57BL/6J-MtgxR7106Btlr/Mmmh
Stock Number:
045198-MU
Citation ID:
RRID:MMRRC_045198-MU
Other Names:
R7106 (G1)
Major Collection:

Strain Information

Myh9
Name: myosin, heavy polypeptide 9, non-muscle
Synonyms: D0Jmb2, E030044M24Rik, NMHC II-A, Myhn-1, Myhn1, myosin IIA, Fltn
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17886
HGNC: HGNC:7579
Homologene: 129835
Dnmt3a
Name: DNA methyltransferase 3A
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 13435
HGNC: HGNC:2978
Homologene: 7294
Kpna4
Name: karyopherin subunit alpha 4
Synonyms: IPOA3, 1110058D08Rik, importin alpha 3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 16649
HGNC: HGNC:6397
Homologene: 20521
Mif4gd
Name: MIF4G domain containing
Synonyms: 1110014L05Rik, 2310075G12Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 69674
Homologene: 41389
Zfp384
Name: zinc finger protein 384
Synonyms: C130073D16Rik, Ciz, Nmp4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 269800
Homologene: 15849
Rapgef2
Name: Rap guanine nucleotide exchange factor (GEF) 2
Synonyms: 5830453M24Rik, Pdzgef1, RA-GEF-1, CNRasGEF, nRapGEP
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 76089
Homologene: 35477
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 11,136,793 bp
  • G to A, chromosome 1 at 66,410,744 bp
  • A to C, chromosome 1 at 93,374,983 bp
  • T to A, chromosome 1 at 164,126,422 bp
  • T to C, chromosome 2 at 76,797,488 bp
  • A to G, chromosome 2 at 153,070,651 bp
  • A to G, chromosome 3 at 64,491,683 bp
  • G to A, chromosome 3 at 69,079,464 bp
  • A to G, chromosome 3 at 79,066,608 bp
  • T to C, chromosome 3 at 103,131,577 bp
  • A to G, chromosome 3 at 145,027,429 bp
  • T to A, chromosome 4 at 22,427,140 bp
  • T to C, chromosome 4 at 49,541,258 bp
  • C to T, chromosome 4 at 68,829,378 bp
  • A to T, chromosome 4 at 73,727,627 bp
  • A to G, chromosome 4 at 98,967,326 bp
  • C to A, chromosome 4 at 133,578,203 bp
  • A to G, chromosome 4 at 136,271,368 bp
  • A to T, chromosome 4 at 139,662,049 bp
  • T to C, chromosome 5 at 33,731,414 bp
  • T to A, chromosome 5 at 107,633,122 bp
  • A to G, chromosome 6 at 30,102,184 bp
  • G to T, chromosome 6 at 57,900,311 bp
  • T to C, chromosome 6 at 122,082,721 bp
  • T to C, chromosome 6 at 125,024,259 bp
  • A to G, chromosome 7 at 4,742,706 bp
  • T to C, chromosome 7 at 19,080,529 bp
  • AATCCGCTTGTGCA to AA, chromosome 7 at 29,895,021 bp
  • T to C, chromosome 7 at 78,843,464 bp
  • A to C, chromosome 7 at 105,749,911 bp
  • G to A, chromosome 7 at 116,418,133 bp
  • T to C, chromosome 7 at 141,522,660 bp
  • A to C, chromosome 8 at 35,825,915 bp
  • T to C, chromosome 8 at 72,724,137 bp
  • G to T, chromosome 9 at 22,236,224 bp
  • A to G, chromosome 9 at 64,132,837 bp
  • G to A, chromosome 9 at 71,725,733 bp
  • A to T, chromosome 10 at 75,638,053 bp
  • A to T, chromosome 10 at 121,301,992 bp
  • A to T, chromosome 11 at 3,998,509 bp
  • T to C, chromosome 11 at 84,522,077 bp
  • G to T, chromosome 11 at 95,055,873 bp
  • G to A, chromosome 11 at 115,611,911 bp
  • A to G, chromosome 12 at 3,897,591 bp
  • A to G, chromosome 12 at 69,984,616 bp
  • A to G, chromosome 12 at 103,765,721 bp
  • T to C, chromosome 12 at 117,961,149 bp
  • C to A, chromosome 13 at 55,050,815 bp
  • C to T, chromosome 13 at 73,914,532 bp
  • A to G, chromosome 14 at 55,588,237 bp
  • A to G, chromosome 15 at 7,172,924 bp
  • A to G, chromosome 15 at 58,116,766 bp
  • A to G, chromosome 15 at 77,775,121 bp
  • T to C, chromosome 16 at 4,705,934 bp
  • T to A, chromosome 16 at 18,399,049 bp
  • T to C, chromosome 16 at 57,140,758 bp
  • T to A, chromosome 17 at 29,376,970 bp
  • T to C, chromosome 17 at 30,741,178 bp
  • T to G, chromosome 17 at 34,852,419 bp
  • A to T, chromosome 17 at 47,391,311 bp
  • C to A, chromosome 17 at 56,658,070 bp
  • A to G, chromosome 17 at 78,586,799 bp
  • A to G, chromosome 19 at 5,718,737 bp
  • G to C, chromosome 19 at 48,705,963 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7106 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045198-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.