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Strain Name:
C57BL/6J-MtgxR4779Btlr/Mmmh
Stock Number:
045240-MU
Citation ID:
RRID:MMRRC_045240-MU
Other Names:
R4779 (G1)
Major Collection:

Strain Information

Dop1b
Name: DOP1 leucine zipper like protein B
Synonyms: 0610038M01Rik, 2610510B01Rik, Dopey2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 70028
VEGA: 16
HGNC: HGNC:1291
Homologene: 21068
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Cwf19l2
Name: CWF19 like cell cycle control factor 2
Synonyms: 3230401L03Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244672
Homologene: 12366
Zdbf2
Name: zinc finger, DBF-type containing 2
Synonyms: 9330107J05Rik, 4930431J08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 73884
Homologene: 52868
Ptpra
Name: protein tyrosine phosphatase receptor type A
Synonyms: RPTRalpha, PTPalpha, PTP[a], Ptpa, RPTPalpha
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 19262
HGNC: HGNC:9664
Homologene: 20621
L3mbtl2
Name: L3MBTL2 polycomb repressive complex 1 subunit
Synonyms: 4732493N06Rik, m4mbt
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 214669
Homologene: 12882
Polk
Name: polymerase (DNA directed), kappa
Synonyms: Dinb1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 27015
VEGA: 13
HGNC: HGNC:9183
Homologene: 32140
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 10,153,733 bp
  • T to A, chromosome 1 at 17,749,607 bp
  • A to G, chromosome 1 at 63,303,238 bp
  • T to C, chromosome 1 at 74,147,663 bp
  • T to C, chromosome 1 at 92,886,723 bp
  • T to C, chromosome 1 at 162,968,838 bp
  • A to G, chromosome 1 at 174,609,895 bp
  • T to A, chromosome 2 at 11,718,306 bp
  • A to T, chromosome 2 at 69,459,715 bp
  • A to G, chromosome 2 at 87,509,597 bp
  • G to T, chromosome 2 at 125,568,892 bp
  • G to A, chromosome 2 at 130,537,617 bp
  • G to T, chromosome 2 at 131,240,365 bp
  • A to G, chromosome 2 at 139,589,589 bp
  • A to T, chromosome 2 at 160,949,557 bp
  • A to G, chromosome 2 at 169,962,681 bp
  • A to G, chromosome 2 at 181,493,460 bp
  • A to G, chromosome 2 at 181,864,547 bp
  • A to T, chromosome 3 at 109,508,794 bp
  • A to G, chromosome 4 at 45,229,865 bp
  • A to T, chromosome 4 at 56,743,632 bp
  • A to T, chromosome 4 at 56,936,468 bp
  • C to T, chromosome 4 at 130,819,065 bp
  • T to C, chromosome 5 at 64,278,046 bp
  • C to T, chromosome 5 at 111,419,660 bp
  • T to C, chromosome 5 at 114,404,717 bp
  • T to A, chromosome 5 at 135,572,957 bp
  • T to C, chromosome 5 at 137,365,702 bp
  • T to A, chromosome 6 at 57,979,026 bp
  • C to T, chromosome 6 at 68,939,910 bp
  • A to G, chromosome 6 at 120,369,099 bp
  • T to A, chromosome 6 at 128,323,120 bp
  • A to G, chromosome 6 at 142,363,700 bp
  • T to A, chromosome 7 at 28,094,937 bp
  • T to C, chromosome 7 at 36,768,972 bp
  • T to C, chromosome 7 at 38,522,256 bp
  • T to A, chromosome 7 at 42,448,031 bp
  • T to A, chromosome 7 at 45,691,471 bp
  • C to T, chromosome 7 at 85,871,715 bp
  • T to C, chromosome 7 at 101,404,367 bp
  • T to A, chromosome 7 at 107,845,548 bp
  • T to G, chromosome 7 at 130,185,193 bp
  • T to C, chromosome 7 at 139,659,815 bp
  • T to A, chromosome 7 at 140,176,450 bp
  • C to T, chromosome 7 at 142,514,283 bp
  • T to C, chromosome 8 at 3,522,838 bp
  • A to G, chromosome 9 at 3,410,035 bp
  • T to A, chromosome 9 at 53,809,729 bp
  • A to T, chromosome 9 at 56,885,808 bp
  • C to T, chromosome 9 at 115,109,530 bp
  • T to A, chromosome 10 at 23,820,869 bp
  • ATGGCGCT to AT, chromosome 10 at 63,100,408 bp
  • C to T, chromosome 10 at 127,173,355 bp
  • T to A, chromosome 11 at 75,620,327 bp
  • A to G, chromosome 11 at 77,686,328 bp
  • G to T, chromosome 11 at 100,770,103 bp
  • T to A, chromosome 11 at 101,178,072 bp
  • C to T, chromosome 11 at 111,724,711 bp
  • A to G, chromosome 11 at 117,806,008 bp
  • A to T, chromosome 12 at 57,729,451 bp
  • A to G, chromosome 12 at 100,403,362 bp
  • A to G, chromosome 13 at 96,496,491 bp
  • A to T, chromosome 13 at 113,368,336 bp
  • A to G, chromosome 14 at 50,361,250 bp
  • A to T, chromosome 14 at 52,231,506 bp
  • G to A, chromosome 14 at 60,753,907 bp
  • A to G, chromosome 14 at 65,730,749 bp
  • T to C, chromosome 15 at 8,218,838 bp
  • A to G, chromosome 15 at 81,682,612 bp
  • A to G, chromosome 15 at 102,224,413 bp
  • T to A, chromosome 16 at 4,849,749 bp
  • C to A, chromosome 16 at 10,511,366 bp
  • T to A, chromosome 16 at 14,410,771 bp
  • T to G, chromosome 16 at 33,719,772 bp
  • C to A, chromosome 16 at 75,947,289 bp
  • T to A, chromosome 16 at 88,611,529 bp
  • G to A, chromosome 16 at 88,873,874 bp
  • T to A, chromosome 16 at 91,414,657 bp
  • T to C, chromosome 16 at 93,757,081 bp
  • C to G, chromosome 17 at 24,510,438 bp
  • T to C, chromosome 17 at 34,022,289 bp
  • T to C, chromosome 17 at 34,193,891 bp
  • T to C, chromosome 17 at 35,052,487 bp
  • G to A, chromosome 17 at 57,296,552 bp
  • T to C, chromosome 18 at 77,991,365 bp
  • C to A, chromosome 19 at 29,958,911 bp
  • G to A, chromosome 19 at 37,417,949 bp
  • T to C, chromosome 19 at 39,877,594 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4779 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045240-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.