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Strain Detail Sheet

Published:
Revised: 03/02/2024

Strain Name:
C57BL/6J-MtgxR7196Btlr/Mmmh
Stock Number:
045275-MU
Citation ID:
RRID:MMRRC_045275-MU
Other Names:
R7196 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Kifc3
Name: kinesin family member C3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 16582
HGNC: HGNC:6326
Homologene: 124421

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Gene Ontology
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KOMP Phenotyping
Monarch
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Ptch2
Name: patched 2
Synonyms: ptc2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 19207
HGNC: HGNC:9586
Homologene: 37842

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Dock5
Name: dedicator of cytokinesis 5
Synonyms: lr2, 1110060D06Rik, rlc
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 68813
VEGA: 14
Homologene: 57016

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Cltc
Name: clathrin heavy chain
Synonyms: CHC
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 67300
HGNC: HGNC:2092
Homologene: 3572

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Rab3gap2
Name: RAB3 GTPase activating protein subunit 2
Synonyms: 1110059F07Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98732
Homologene: 40842

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Tnpo2
Name: transportin 2 (importin 3, karyopherin beta 2b)
Synonyms: Kpnb2b, TRN2, 1110034O24Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 212999
Homologene: 8381

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Cnn3
Name: calponin 3, acidic
Synonyms: 1600014M03Rik, Calpo3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 71994
HGNC: HGNC:2157
Homologene: 37533

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 53,684,841 bp
  • T to A, chromosome 1 at 117,986,061 bp
  • T to C, chromosome 1 at 171,344,435 bp
  • G to T, chromosome 1 at 174,026,543 bp
  • T to C, chromosome 1 at 185,281,667 bp
  • A to G, chromosome 1 at 193,035,628 bp
  • A to T, chromosome 2 at 17,452,518 bp
  • A to C, chromosome 2 at 19,493,995 bp
  • A to T, chromosome 2 at 35,086,429 bp
  • A to G, chromosome 2 at 66,090,924 bp
  • C to A, chromosome 2 at 76,752,042 bp
  • T to G, chromosome 2 at 111,664,697 bp
  • T to G, chromosome 3 at 64,715,777 bp
  • C to A, chromosome 3 at 97,051,106 bp
  • A to C, chromosome 3 at 104,049,168 bp
  • T to C, chromosome 3 at 107,753,898 bp
  • G to A, chromosome 3 at 121,454,788 bp
  • A to T, chromosome 3 at 152,384,191 bp
  • A to T, chromosome 4 at 19,608,938 bp
  • A to G, chromosome 4 at 25,250,669 bp
  • A to C, chromosome 4 at 46,119,955 bp
  • G to A, chromosome 4 at 65,323,891 bp
  • T to A, chromosome 4 at 96,215,414 bp
  • T to G, chromosome 4 at 101,102,859 bp
  • T to G, chromosome 4 at 117,114,749 bp
  • T to A, chromosome 4 at 123,323,372 bp
  • A to T, chromosome 5 at 21,486,303 bp
  • A to T, chromosome 5 at 138,239,324 bp
  • A to T, chromosome 6 at 5,423,356 bp
  • A to G, chromosome 6 at 38,329,272 bp
  • G to T, chromosome 6 at 140,543,922 bp
  • T to C, chromosome 7 at 3,163,958 bp
  • G to A, chromosome 7 at 5,093,381 bp
  • T to C, chromosome 7 at 17,154,956 bp
  • G to A, chromosome 7 at 19,223,164 bp
  • A to T, chromosome 7 at 86,269,441 bp
  • G to A, chromosome 7 at 101,828,786 bp
  • A to T, chromosome 7 at 103,442,997 bp
  • A to G, chromosome 7 at 107,074,728 bp
  • G to A, chromosome 7 at 143,358,741 bp
  • A to G, chromosome 8 at 11,398,693 bp
  • G to A, chromosome 8 at 46,635,463 bp
  • G to A, chromosome 8 at 85,047,137 bp
  • A to G, chromosome 8 at 95,106,611 bp
  • G to T, chromosome 8 at 106,425,148 bp
  • C to A, chromosome 8 at 122,638,990 bp
  • G to A, chromosome 9 at 7,147,715 bp
  • C to T, chromosome 9 at 7,476,017 bp
  • G to T, chromosome 9 at 13,244,157 bp
  • A to T, chromosome 9 at 18,755,590 bp
  • A to G, chromosome 9 at 20,314,198 bp
  • T to A, chromosome 9 at 37,402,705 bp
  • A to T, chromosome 9 at 56,620,619 bp
  • AGCTTCCCTGCTT to AGCTT, chromosome 9 at 57,258,222 bp
  • A to T, chromosome 9 at 85,743,656 bp
  • T to A, chromosome 9 at 110,851,465 bp
  • A to G, chromosome 10 at 44,456,992 bp
  • A to T, chromosome 10 at 60,307,980 bp
  • T to A, chromosome 10 at 81,144,600 bp
  • C to A, chromosome 10 at 89,714,922 bp
  • C to T, chromosome 10 at 93,733,714 bp
  • T to C, chromosome 10 at 121,840,848 bp
  • A to G, chromosome 11 at 73,517,131 bp
  • T to C, chromosome 11 at 86,706,831 bp
  • C to T, chromosome 11 at 99,387,545 bp
  • T to C, chromosome 11 at 102,438,346 bp
  • C to A, chromosome 11 at 104,633,612 bp
  • A to G, chromosome 12 at 7,983,893 bp
  • G to A, chromosome 12 at 81,995,538 bp
  • A to T, chromosome 12 at 100,595,864 bp
  • C to T, chromosome 12 at 109,592,787 bp
  • T to G, chromosome 13 at 24,704,825 bp
  • T to C, chromosome 13 at 59,533,180 bp
  • C to A, chromosome 13 at 65,044,826 bp
  • A to T, chromosome 13 at 100,300,158 bp
  • T to A, chromosome 14 at 20,588,370 bp
  • A to G, chromosome 14 at 57,873,891 bp
  • G to A, chromosome 14 at 67,756,470 bp
  • T to A, chromosome 14 at 101,896,500 bp
  • T to C, chromosome 14 at 105,298,038 bp
  • T to A, chromosome 15 at 66,831,571 bp
  • G to T, chromosome 15 at 99,722,055 bp
  • A to T, chromosome 16 at 18,776,880 bp
  • A to G, chromosome 16 at 32,273,802 bp
  • G to A, chromosome 16 at 37,588,716 bp
  • A to T, chromosome 17 at 24,739,582 bp
  • C to T, chromosome 17 at 33,933,429 bp
  • A to G, chromosome 17 at 37,211,193 bp
  • A to G, chromosome 17 at 40,602,365 bp
  • C to T, chromosome 17 at 88,985,469 bp
  • T to C, chromosome 18 at 20,466,480 bp
  • T to A, chromosome 18 at 43,329,845 bp
  • T to C, chromosome 18 at 67,562,502 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7196 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
045275-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.