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Strain Name:
C57BL/6J-MtgxR7228Btlr/Mmmh
Stock Number:
045300-MU
Citation ID:
RRID:MMRRC_045300-MU
Other Names:
R7228 (G1)
Major Collection:

Strain Information

Matr3
Name: matrin 3
Synonyms: D030046F20Rik, 2810017I02Rik, 1110061A14Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 17184
HGNC: HGNC:6912
Homologene: 7830
Map2k5
Name: mitogen-activated protein kinase kinase 5
Synonyms: MEK5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 23938
VEGA: 9
HGNC: HGNC:6845
Homologene: 115933
Notch2
Name: notch 2
Synonyms: Motch B, N2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18129
HGNC: HGNC:7882
Homologene: 7865
Amigo1
Name: adhesion molecule with Ig like domain 1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229715
Homologene: 46421
Adcy10
Name: adenylate cyclase 10
Synonyms: soluble adenylyl cyclase, sAC, 4930431D04Rik, Sacy
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 271639
Homologene: 10188
Slit3
Name: slit guidance ligand 3
Synonyms: Slit1, b2b2362.1Clo
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20564
Homologene: 2303
Prrc2b
Name: proline-rich coiled-coil 2B
Synonyms: 5830434P21Rik, Bat2l
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227723
Homologene: 106649
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 33,803,394 bp
  • A to T, chromosome 1 at 60,109,394 bp
  • A to G, chromosome 1 at 136,071,059 bp
  • G to A, chromosome 1 at 156,434,238 bp
  • A to C, chromosome 1 at 165,510,272 bp
  • T to C, chromosome 1 at 181,510,967 bp
  • C to A, chromosome 2 at 32,214,306 bp
  • T to A, chromosome 2 at 82,992,307 bp
  • T to A, chromosome 2 at 105,792,302 bp
  • T to C, chromosome 2 at 112,861,852 bp
  • C to T, chromosome 2 at 118,457,157 bp
  • T to C, chromosome 2 at 172,551,572 bp
  • A to T, chromosome 2 at 180,954,569 bp
  • T to C, chromosome 3 at 79,069,218 bp
  • T to A, chromosome 3 at 82,033,274 bp
  • T to C, chromosome 3 at 82,842,533 bp
  • T to C, chromosome 3 at 84,192,181 bp
  • T to A, chromosome 3 at 93,073,686 bp
  • T to A, chromosome 3 at 98,137,317 bp
  • T to C, chromosome 3 at 108,187,230 bp
  • C to T, chromosome 3 at 138,957,467 bp
  • T to A, chromosome 4 at 14,775,027 bp
  • G to T, chromosome 4 at 88,722,305 bp
  • G to A, chromosome 4 at 155,465,050 bp
  • T to A, chromosome 5 at 8,976,051 bp
  • T to C, chromosome 5 at 104,440,445 bp
  • C to A, chromosome 7 at 62,419,667 bp
  • A to T, chromosome 7 at 107,037,100 bp
  • T to C, chromosome 7 at 126,953,725 bp
  • T to G, chromosome 8 at 85,843,007 bp
  • T to C, chromosome 9 at 51,291,656 bp
  • A to T, chromosome 9 at 54,714,285 bp
  • C to T, chromosome 9 at 63,358,022 bp
  • C to A, chromosome 9 at 106,176,672 bp
  • C to G, chromosome 9 at 109,724,944 bp
  • T to C, chromosome 9 at 116,109,943 bp
  • A to G, chromosome 10 at 82,151,271 bp
  • A to G, chromosome 10 at 127,045,283 bp
  • T to C, chromosome 11 at 6,598,412 bp
  • T to A, chromosome 11 at 9,297,653 bp
  • T to A, chromosome 11 at 35,599,418 bp
  • T to A, chromosome 11 at 49,134,879 bp
  • A to T, chromosome 12 at 85,235,656 bp
  • A to G, chromosome 12 at 87,957,949 bp
  • C to T, chromosome 12 at 111,305,093 bp
  • A to T, chromosome 12 at 113,932,361 bp
  • A to G, chromosome 13 at 15,724,502 bp
  • C to A, chromosome 14 at 4,252,444 bp
  • T to A, chromosome 14 at 66,053,912 bp
  • T to A, chromosome 14 at 101,896,535 bp
  • A to G, chromosome 14 at 103,049,329 bp
  • T to C, chromosome 15 at 12,372,973 bp
  • T to A, chromosome 15 at 12,458,145 bp
  • A to G, chromosome 15 at 99,582,124 bp
  • A to G, chromosome 17 at 24,658,091 bp
  • A to G, chromosome 17 at 32,491,829 bp
  • C to T, chromosome 17 at 52,956,716 bp
  • T to A, chromosome 17 at 65,717,553 bp
  • T to A, chromosome 18 at 15,062,412 bp
  • T to C, chromosome 18 at 35,562,484 bp
  • T to C, chromosome 18 at 43,477,509 bp
  • T to C, chromosome 18 at 57,189,589 bp
  • A to G, chromosome 19 at 15,969,197 bp
  • C to A, chromosome 19 at 39,881,166 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7228 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045300-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.