Strain Name:
C57BL/6J-MtgxR7228Btlr/Mmmh
Stock Number:
045300-MU
Citation ID:
RRID:MMRRC_045300-MU
Other Names:
R7228 (G1)
Major Collection:

Strain Information

Matr3
Name: matrin 3
Synonyms: 2810017I02Rik, 1110061A14Rik, D030046F20Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 17184
HGNC: HGNC:6912
Homologene: 7830
Map2k5
Name: mitogen-activated protein kinase kinase 5
Synonyms: MEK5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 23938
VEGA: 9
HGNC: HGNC:6845
Homologene: 115933
Notch2
Name: notch 2
Synonyms: Motch B, N2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18129
HGNC: HGNC:7882
Homologene: 7865
Amigo1
Name: adhesion molecule with Ig like domain 1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229715
Homologene: 46421
Adcy10
Name: adenylate cyclase 10
Synonyms: soluble adenylyl cyclase, Sacy, 4930431D04Rik, sAC
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 271639
Homologene: 10188
Slit3
Name: slit guidance ligand 3
Synonyms: b2b2362.1Clo, Slit1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20564
Homologene: 2303
Prrc2b
Name: proline-rich coiled-coil 2B
Synonyms: Bat2l, 5830434P21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227723
Homologene: 106649
Wdr82
Name: WD repeat domain containing 82
Synonyms: 9430077D24Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 77305
Homologene: 42951
Pdzd2
Name: PDZ domain containing 2
Synonyms: LOC223364, 4930537L06Rik, Pdzk3, A930022H17Rik, Gm21706
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 68070
Homologene: 23393
Rapgef2
Name: Rap guanine nucleotide exchange factor (GEF) 2
Synonyms: 5830453M24Rik, Pdzgef1, CNRasGEF, nRapGEP, RA-GEF-1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 76089
Homologene: 35477
Cdc42bpb
Name: CDC42 binding protein kinase beta
Synonyms: DMPK-like
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217866
VEGA: 12
HGNC: HGNC:1738
Homologene: 55945
Eif2ak4
Name: eukaryotic translation initiation factor 2 alpha kinase 4
Synonyms: GCN2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 27103
Homologene: 40891
Phkb
Name: phosphorylase kinase beta
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 102093
HGNC: HGNC:8927
Homologene: 247
Cep78
Name: centrosomal protein 78
Synonyms: 5730599I05Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 208518
VEGA: 19
Homologene: 11030
Mlh3
Name: mutL homolog 3
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217716
VEGA: 12
HGNC: HGNC:7128
Homologene: 91153
Crot
Name: carnitine O-octanoyltransferase
Synonyms: 1200003H03Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 74114
HGNC: HGNC:2366
Homologene: 10899
Tfap2c
Name: transcription factor AP-2, gamma
Synonyms: Stra2, Ap-2.2, AP2gamma, Tcfap2c
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21420
Homologene: 2423
Rab31
Name: RAB31, member RAS oncogene family
Synonyms: 1700093E07Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 106572
VEGA: 17
HGNC: HGNC:9771
Homologene: 5001
Soat1
Name: sterol O-acyltransferase 1
Synonyms: hid, Acact, 8430426K15Rik, ACAT-1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 20652
Homologene: 2333
Mettl1
Name: methyltransferase 1, tRNA methylguanosine
Synonyms: 2810012D02Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17299
HGNC: HGNC:7030
Homologene: 3918
Sez6l2
Name: seizure related 6 homolog like 2
Synonyms: Psk1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233878
Homologene: 8237
Trim2
Name: tripartite motif-containing 2
Synonyms: narf, neural activity-related ring finger protein
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 80890
Homologene: 22882
Lmo7
Name: LIM domain only 7
Synonyms: LOC380928, FBXO20
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 380928
HGNC: HGNC:6646
Homologene: 83924
Zfp451
Name: zinc finger protein 451
Synonyms: Kiaa0576-hp, 4933435G09Rik, 4930515K21Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98403
Homologene: 9188
AU041133
Name: expressed sequence AU041133
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216177
Homologene: 87560
Rap1gds1
Name: RAP1, GTP-GDP dissociation stimulator 1
Synonyms: GDS1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229877
HGNC: HGNC:9859
Homologene: 41422
Spp1
Name: secreted phosphoprotein 1
Synonyms: bone sialoprotein 1, Spp-1, 44kDa bone phosphoprotein, 2ar, osteopontin, minopontin, Ric, Opn, BNSP, ETA-1, OP, osteopontin-like protein, Apl-1, Opnl
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20750
Homologene: 20156
Abca13
Name: ATP-binding cassette, sub-family A member 13
Synonyms: A930002G16Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 268379
Homologene: 27991
Cfap74
Name: cilia and flagella associated protein 74
Synonyms: 2010015L04Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 544678
Homologene: 129584
Gucy1b1
Name: guanylate cyclase 1, soluble, beta 1
Synonyms: Gucy1b3, beta 1 sGC
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 54195
HGNC: HGNC:4687
Homologene: 664
Fsip2
Name: fibrous sheath-interacting protein 2
Synonyms: OTTMUSG00000013335
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241516
Homologene: 110349
Megf10
Name: multiple EGF-like-domains 10
Synonyms: 3000002B06Rik, LOC240312
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 70417
Homologene: 23771
Ryr3
Name: ryanodine receptor 3
Synonyms: calcium release channel isoform 3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20192
Homologene: 68151
Fbxw26
Name: F-box and WD-40 domain protein 26
Synonyms: Gm5163
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 382109
Homologene: 110776
Cacna1s
Name: calcium channel, voltage-dependent, L type, alpha 1S subunit
Synonyms: Cchl1a3, Cav1.1, muscle dysgenesis, DHPR alpha1s, mdg, sj, fmd
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12292
HGNC: HGNC:1397
Homologene: 37257
Rbm46
Name: RNA binding motif protein 46
Synonyms: ENSMUSG00000033882, LOC329687
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 633285
Homologene: 17016
Acod1
Name: aconitate decarboxylase 1
Synonyms: Irg1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 16365
Homologene: 35405
Ccdc121rt1
Name: coiled-coil domain containing 121, retrogene 1
Synonyms: Ccdc121, 6530421E24Rik, LOC329308
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 403180
Homologene: 136217
Adam2
Name: a disintegrin and metallopeptidase domain 2
Synonyms: Ftnb, Ph30-beta, fertilin beta
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 11495
VEGA: 14
HGNC: HGNC:198
Homologene: 1127
Gli3
Name: GLI-Kruppel family member GLI3
Synonyms: brachyphalangy, Bph
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 14634
HGNC: HGNC:4319
Homologene: 139
Elp4
Name: elongator acetyltransferase complex subunit 4
Synonyms: A330107A17Rik, Paxneb
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 77766
HGNC: HGNC:1171
Homologene: 32433
Kctd1
Name: potassium channel tetramerisation domain containing 1
Synonyms: 4933402K10Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 106931
VEGA: 18
Homologene: 65999
Cyp4f39
Name: cytochrome P450, family 4, subfamily f, polypeptide 39
Synonyms: 4732474A20Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 320997
VEGA: 17
Homologene: 69814
Or2v1
Name: olfactory receptor family 2 subfamily V member 1
Synonyms: GA_x6K02T2QP88-6300500-6299553, Olfr56, IF7, MOR276-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18356
HGNC: HGNC:8280
Homologene: 22608
Kcnh8
Name: potassium voltage-gated channel, subfamily H (eag-related), member 8
Synonyms: ELK1, Kv12.1, C130090D05Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 211468
Homologene: 14332
Nacad
Name: NAC alpha domain containing
Synonyms: mKIAA0363
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 192950
Homologene: 135717
Gm2042
Name: predicted gene 2042
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 100039087
Homologene: 103830
Carf
Name: calcium response factor
Synonyms: Als2cr8
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 241066
Homologene: 11689
Or10a5
Name: olfactory receptor family 10 subfamily A member 5
Synonyms: MOR263-1, P3, Olfr713, GA_x6K02T2PBJ9-9415724-9416677
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 259036
Homologene: 17470
Cyp2c69
Name: cytochrome P450, family 2, subfamily c, polypeptide 69
Synonyms: AI098658
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 100043108
VEGA: 19
Homologene: 74936
Mkrn3
Name: makorin, ring finger protein, 3
Synonyms: D7H15S9-1, Zfp127
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22652
HGNC: HGNC:7114
Homologene: 4143
Lrrc69
Name: leucine rich repeat containing 69
Synonyms: 1700034K16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 73314
Homologene: 78018
Tgfbr2
Name: transforming growth factor, beta receptor II
Synonyms: TBR-II, TbetaRII, TbetaR-II, 1110020H15Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 21813
VEGA: 9
Homologene: 2435
Nkain4
Name: Na+/K+ transporting ATPase interacting 4
Synonyms: C030019F02Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 58237
Homologene: 10978
Eif3j2
Name: eukaryotic translation initiation factor 3, subunit J2
Synonyms: Gm9781
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 100042807
HGNC: HGNC:3270
Homologene: 37845
Aqp2
Name: aquaporin 2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 11827
VEGA: 15
HGNC: HGNC:634
Homologene: 20137
Pou2af2
Name: POU domain, class 2, associating factor 2
Synonyms: OCA-B homolog, 1810046K07Rik, Oca-t1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 69809
Homologene: 18881
Tdpoz8
Name: TD and POZ domain containing 8
Synonyms: Gm4858
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229571
Homologene: 130121
Npw
Name: neuropeptide W
Synonyms: LOC381073
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 381073
Homologene: 17727
Dnaja4
Name: DnaJ heat shock protein family (Hsp40) member A4
Synonyms: Hsj4, 1110021L12Rik, Dj4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 58233
Homologene: 23110
Ighv14-1
Name: immunoglobulin heavy variable 14-1
Synonyms: Gm16996
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 780801
Gm3138
Name: predicted gene 3138
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 100041100
Gm26566
Name: predicted gene, 26566
Type: Gene
Species: Mouse
Chromosome: 4
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 33,803,394 bp
  • A to T, chromosome 1 at 60,109,394 bp
  • A to G, chromosome 1 at 136,071,059 bp
  • G to A, chromosome 1 at 156,434,238 bp
  • A to C, chromosome 1 at 165,510,272 bp
  • T to C, chromosome 1 at 181,510,967 bp
  • C to A, chromosome 2 at 32,214,306 bp
  • T to A, chromosome 2 at 82,992,307 bp
  • T to A, chromosome 2 at 105,792,302 bp
  • T to C, chromosome 2 at 112,861,852 bp
  • C to T, chromosome 2 at 118,457,157 bp
  • T to C, chromosome 2 at 172,551,572 bp
  • A to T, chromosome 2 at 180,954,569 bp
  • T to C, chromosome 3 at 79,069,218 bp
  • T to A, chromosome 3 at 82,033,274 bp
  • T to C, chromosome 3 at 82,842,533 bp
  • T to C, chromosome 3 at 84,192,181 bp
  • T to A, chromosome 3 at 93,073,686 bp
  • T to A, chromosome 3 at 98,137,317 bp
  • T to C, chromosome 3 at 108,187,230 bp
  • C to T, chromosome 3 at 138,957,467 bp
  • T to A, chromosome 4 at 14,775,027 bp
  • G to T, chromosome 4 at 88,722,305 bp
  • G to A, chromosome 4 at 155,465,050 bp
  • T to A, chromosome 5 at 8,976,051 bp
  • T to C, chromosome 5 at 104,440,445 bp
  • C to A, chromosome 7 at 62,419,667 bp
  • A to T, chromosome 7 at 107,037,100 bp
  • T to C, chromosome 7 at 126,953,725 bp
  • T to G, chromosome 8 at 85,843,007 bp
  • T to C, chromosome 9 at 51,291,656 bp
  • A to T, chromosome 9 at 54,714,285 bp
  • C to T, chromosome 9 at 63,358,022 bp
  • C to A, chromosome 9 at 106,176,672 bp
  • C to G, chromosome 9 at 109,724,944 bp
  • T to C, chromosome 9 at 116,109,943 bp
  • A to G, chromosome 10 at 82,151,271 bp
  • A to G, chromosome 10 at 127,045,283 bp
  • T to C, chromosome 11 at 6,598,412 bp
  • T to A, chromosome 11 at 9,297,653 bp
  • T to A, chromosome 11 at 35,599,418 bp
  • T to A, chromosome 11 at 49,134,879 bp
  • A to T, chromosome 12 at 85,235,656 bp
  • A to G, chromosome 12 at 87,957,949 bp
  • C to T, chromosome 12 at 111,305,093 bp
  • A to T, chromosome 12 at 113,932,361 bp
  • A to G, chromosome 13 at 15,724,502 bp
  • C to A, chromosome 14 at 4,252,444 bp
  • T to A, chromosome 14 at 66,053,912 bp
  • T to A, chromosome 14 at 101,896,535 bp
  • A to G, chromosome 14 at 103,049,329 bp
  • T to C, chromosome 15 at 12,372,973 bp
  • T to A, chromosome 15 at 12,458,145 bp
  • A to G, chromosome 15 at 99,582,124 bp
  • A to G, chromosome 17 at 24,658,091 bp
  • A to G, chromosome 17 at 32,491,829 bp
  • C to T, chromosome 17 at 52,956,716 bp
  • T to A, chromosome 17 at 65,717,553 bp
  • T to A, chromosome 18 at 15,062,412 bp
  • T to C, chromosome 18 at 35,562,484 bp
  • T to C, chromosome 18 at 43,477,509 bp
  • T to C, chromosome 18 at 57,189,589 bp
  • A to G, chromosome 19 at 15,969,197 bp
  • C to A, chromosome 19 at 39,881,166 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7228 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045300-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.