Loading...

Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR7326Btlr/Mmmh
Stock Number:
045378-MU
Citation ID:
RRID:MMRRC_045378-MU
Other Names:
R7326 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Frem2
Name: Fras1 related extracellular matrix protein 2
Synonyms: 8430406N05Rik, 6030440P17Rik, my, ne, b2b1562Clo
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 242022
Homologene: 18454

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Nrcam
Name: neuronal cell adhesion molecule
Synonyms: Bravo, C030017F07Rik, C130076O07Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 319504
VEGA: 12
HGNC: HGNC:7994
Homologene: 21041

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Ptprf
Name: protein tyrosine phosphatase receptor type F
Synonyms: LAR, RPTP-LAR
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 19268
HGNC: HGNC:9670
Homologene: 20623

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Rnf38
Name: ring finger protein 38
Synonyms: Oip1, 1700065B19Rik, 2610202O07Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 73469
Homologene: 32550

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Add1
Name: adducin 1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 11518
HGNC: HGNC:243
Homologene: 22758

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Fgfr1
Name: fibroblast growth factor receptor 1
Synonyms: Flt-2, Fgfr-1, Hspy, Eask, FGFR-I, Fr1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 14182
HGNC: HGNC:3688
Homologene: 69065

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Srgap2
Name: SLIT-ROBO Rho GTPase activating protein 2
Synonyms: FBP2, 9930124L22Rik, Fnbp2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14270
Homologene: 52683

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 45,442,867 bp
  • A to T, chromosome 1 at 131,291,613 bp
  • G to A, chromosome 1 at 171,174,465 bp
  • A to G, chromosome 1 at 180,569,100 bp
  • A to G, chromosome 1 at 181,598,403 bp
  • G to A, chromosome 1 at 191,164,353 bp
  • A to T, chromosome 1 at 191,719,453 bp
  • C to T, chromosome 2 at 3,226,472 bp
  • A to G, chromosome 2 at 26,439,717 bp
  • A to T, chromosome 2 at 74,675,246 bp
  • A to T, chromosome 2 at 79,332,605 bp
  • A to T, chromosome 2 at 85,414,444 bp
  • A to G, chromosome 2 at 86,198,573 bp
  • G to A, chromosome 2 at 109,996,629 bp
  • A to T, chromosome 2 at 111,983,327 bp
  • A to G, chromosome 2 at 120,430,599 bp
  • A to T, chromosome 2 at 121,160,988 bp
  • G to A, chromosome 2 at 127,761,217 bp
  • T to C, chromosome 3 at 49,756,860 bp
  • G to C, chromosome 3 at 53,654,753 bp
  • T to A, chromosome 3 at 88,555,910 bp
  • A to C, chromosome 3 at 108,394,995 bp
  • A to T, chromosome 3 at 148,846,870 bp
  • C to A, chromosome 4 at 44,158,989 bp
  • G to T, chromosome 4 at 59,960,046 bp
  • T to A, chromosome 4 at 116,980,834 bp
  • A to G, chromosome 4 at 118,231,669 bp
  • T to A, chromosome 4 at 140,832,404 bp
  • C to T, chromosome 4 at 149,996,350 bp
  • T to A, chromosome 5 at 4,045,930 bp
  • T to C, chromosome 5 at 8,934,226 bp
  • T to A, chromosome 5 at 18,289,551 bp
  • A to G, chromosome 5 at 22,523,584 bp
  • A to G, chromosome 5 at 34,619,371 bp
  • A to T, chromosome 5 at 35,064,522 bp
  • A to T, chromosome 5 at 63,800,409 bp
  • T to C, chromosome 5 at 96,077,299 bp
  • T to C, chromosome 5 at 106,638,277 bp
  • C to A, chromosome 5 at 115,082,335 bp
  • C to T, chromosome 5 at 127,564,059 bp
  • A to G, chromosome 5 at 129,862,710 bp
  • A to G, chromosome 5 at 130,187,351 bp
  • T to A, chromosome 5 at 134,986,983 bp
  • G to A, chromosome 5 at 138,175,019 bp
  • A to T, chromosome 6 at 34,904,626 bp
  • G to T, chromosome 6 at 40,544,895 bp
  • G to T, chromosome 6 at 55,336,851 bp
  • A to G, chromosome 6 at 71,846,369 bp
  • A to G, chromosome 6 at 118,430,209 bp
  • A to T, chromosome 6 at 132,777,906 bp
  • T to A, chromosome 7 at 27,329,755 bp
  • G to T, chromosome 7 at 29,210,759 bp
  • T to A, chromosome 7 at 35,385,463 bp
  • T to A, chromosome 7 at 44,859,734 bp
  • A to G, chromosome 7 at 86,321,574 bp
  • G to T, chromosome 7 at 105,617,800 bp
  • G to A, chromosome 7 at 105,720,930 bp
  • A to T, chromosome 7 at 108,880,816 bp
  • A to T, chromosome 7 at 139,978,545 bp
  • T to C, chromosome 8 at 3,641,151 bp
  • T to G, chromosome 8 at 25,573,839 bp
  • T to C, chromosome 8 at 71,988,654 bp
  • T to A, chromosome 8 at 83,722,471 bp
  • T to C, chromosome 8 at 105,312,898 bp
  • A to T, chromosome 8 at 105,449,769 bp
  • G to A, chromosome 8 at 125,887,083 bp
  • A to G, chromosome 9 at 13,621,607 bp
  • T to A, chromosome 9 at 18,585,013 bp
  • A to G, chromosome 9 at 19,121,669 bp
  • T to C, chromosome 9 at 20,502,095 bp
  • A to T, chromosome 9 at 35,685,972 bp
  • T to C, chromosome 9 at 77,164,842 bp
  • C to A, chromosome 9 at 108,054,066 bp
  • A to T, chromosome 10 at 7,777,850 bp
  • A to T, chromosome 10 at 10,400,574 bp
  • T to A, chromosome 10 at 36,833,194 bp
  • T to A, chromosome 10 at 75,642,565 bp
  • A to T, chromosome 10 at 80,311,740 bp
  • A to G, chromosome 10 at 81,181,282 bp
  • A to T, chromosome 10 at 116,937,633 bp
  • C to A, chromosome 11 at 32,194,389 bp
  • T to A, chromosome 11 at 55,282,304 bp
  • A to G, chromosome 11 at 79,564,943 bp
  • G to A, chromosome 11 at 94,493,780 bp
  • T to A, chromosome 11 at 99,864,881 bp
  • T to C, chromosome 11 at 106,072,617 bp
  • A to G, chromosome 12 at 4,632,985 bp
  • C to T, chromosome 12 at 44,564,026 bp
  • C to A, chromosome 12 at 55,709,004 bp
  • G to A, chromosome 12 at 84,457,954 bp
  • T to A, chromosome 13 at 11,738,194 bp
  • T to C, chromosome 13 at 23,585,923 bp
  • C to T, chromosome 13 at 38,192,883 bp
  • C to A, chromosome 13 at 49,369,609 bp
  • A to T, chromosome 15 at 78,013,320 bp
  • A to G, chromosome 15 at 85,852,861 bp
  • G to C, chromosome 15 at 95,864,244 bp
  • G to T, chromosome 15 at 101,141,072 bp
  • A to G, chromosome 16 at 4,632,048 bp
  • G to A, chromosome 16 at 10,512,288 bp
  • A to T, chromosome 16 at 22,548,690 bp
  • A to T, chromosome 16 at 91,688,711 bp
  • A to G, chromosome 16 at 94,692,043 bp
  • C to A, chromosome 17 at 28,806,293 bp
  • T to C, chromosome 17 at 35,886,160 bp
  • T to A, chromosome 18 at 62,952,188 bp
  • G to T, chromosome 18 at 68,339,668 bp
  • A to T, chromosome 18 at 84,559,042 bp
  • A to T, chromosome 19 at 3,507,325 bp
  • T to C, chromosome 19 at 4,184,898 bp
  • G to T, chromosome 19 at 10,456,778 bp
  • A to G, chromosome 19 at 46,324,454 bp
  • A to T, chromosome 19 at 55,302,755 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7326 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
045378-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.