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Strain Name:
C57BL/6J-MtgxR6982Btlr/Mmmh
Stock Number:
045383-MU
Citation ID:
RRID:MMRRC_045383-MU
Other Names:
R6982 (G1)
Major Collection:

Strain Information

Fezf2
Name: Fez family zinc finger 2
Synonyms: forebrain embryonic zinc finger, Fez, Fezl, Zfp312
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 54713
VEGA: 14
Homologene: 9957
Fam3c
Name: FAM3 metabolism regulating signaling molecule C
Synonyms: Fam3c, D6Wsu176e, Ilei
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 27999
Homologene: 8926
Clybl
Name: citrate lyase beta like
Synonyms: Clb, 2310014M14Rik, 0610033J05Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 69634
VEGA: 14
Homologene: 49925
Ssbp4
Name: single stranded DNA binding protein 4
Synonyms: 1210002E11Rik, Ssdp4
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 76900
Homologene: 41881
Wdr59
Name: WD repeat domain 59
Synonyms: 5430401O09Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 319481
Homologene: 38685
Ambra1
Name: autophagy/beclin 1 regulator 1
Synonyms: 2310079H06Rik, D030051N19Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228361
Homologene: 18204
Tcf3
Name: transcription factor 3
Synonyms: ALF2, E2A, E47, E12, Pan2, Pan1, A1, Tcfe2a, bHLHb21
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 21423
Homologene: 2408
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 7,147,682 bp
  • T to C, chromosome 1 at 164,256,859 bp
  • A to T, chromosome 2 at 70,426,065 bp
  • A to G, chromosome 2 at 91,190,759 bp
  • G to A, chromosome 2 at 91,917,473 bp
  • T to C, chromosome 2 at 180,996,706 bp
  • A to G, chromosome 3 at 5,403,830 bp
  • A to G, chromosome 3 at 107,299,273 bp
  • A to G, chromosome 3 at 142,630,085 bp
  • G to T, chromosome 3 at 145,315,046 bp
  • T to C, chromosome 3 at 154,580,230 bp
  • A to T, chromosome 4 at 117,889,796 bp
  • A to T, chromosome 4 at 118,153,439 bp
  • A to G, chromosome 4 at 123,325,141 bp
  • A to T, chromosome 4 at 141,096,873 bp
  • A to T, chromosome 5 at 37,124,941 bp
  • T to C, chromosome 5 at 38,672,905 bp
  • C to A, chromosome 5 at 100,092,821 bp
  • G to T, chromosome 6 at 22,322,301 bp
  • A to T, chromosome 6 at 73,469,544 bp
  • G to A, chromosome 6 at 124,517,725 bp
  • T to A, chromosome 6 at 124,860,956 bp
  • A to T, chromosome 6 at 125,176,736 bp
  • G to A, chromosome 6 at 125,992,893 bp
  • A to T, chromosome 7 at 12,321,836 bp
  • C to A, chromosome 7 at 82,515,063 bp
  • T to C, chromosome 7 at 90,396,564 bp
  • T to C, chromosome 8 at 70,608,165 bp
  • A to T, chromosome 8 at 111,460,813 bp
  • T to A, chromosome 9 at 4,384,061 bp
  • T to C, chromosome 9 at 39,394,322 bp
  • T to C, chromosome 9 at 65,279,805 bp
  • A to G, chromosome 9 at 92,482,743 bp
  • A to T, chromosome 9 at 96,560,750 bp
  • A to T, chromosome 9 at 101,023,296 bp
  • T to C, chromosome 10 at 54,074,723 bp
  • A to T, chromosome 10 at 80,417,550 bp
  • T to C, chromosome 10 at 81,420,800 bp
  • C to A, chromosome 12 at 104,317,297 bp
  • T to C, chromosome 13 at 22,501,747 bp
  • T to A, chromosome 13 at 70,768,520 bp
  • G to T, chromosome 14 at 12,343,645 bp
  • A to G, chromosome 14 at 26,799,076 bp
  • A to G, chromosome 14 at 75,084,771 bp
  • A to G, chromosome 14 at 99,313,235 bp
  • G to A, chromosome 14 at 122,401,947 bp
  • A to T, chromosome 15 at 44,566,268 bp
  • T to C, chromosome 17 at 18,452,061 bp
  • T to A, chromosome 17 at 30,767,925 bp
  • T to C, chromosome 17 at 43,539,035 bp
  • T to A, chromosome 17 at 46,434,637 bp
  • C to T, chromosome 17 at 58,092,252 bp
  • T to A, chromosome 17 at 87,307,009 bp
  • G to A, chromosome 18 at 43,977,725 bp
  • T to C, chromosome 18 at 44,010,042 bp
  • C to T, chromosome 19 at 7,682,969 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6982 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045383-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.