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Strain Name:
C57BL/6J-MtgxR7327Btlr/Mmmh
Stock Number:
045420-MU
Citation ID:
RRID:MMRRC_045420-MU
Other Names:
R7327 (G1)
Major Collection:

Strain Information

Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Map7
Name: microtubule-associated protein 7
Synonyms: E-MAP-115, mste, ste, mshi, Mtap7
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17761
VEGA: 10
HGNC: HGNC:6869
Homologene: 20851
Orc1
Name: origin recognition complex, subunit 1
Synonyms: MmORC1, Orc1l
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18392
HGNC: HGNC:8487
Homologene: 31221
Rad54l2
Name: RAD54 like 2 (S. cerevisiae)
Synonyms: Arip4, G630026H09Rik, Srisnf2l
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 81000
Homologene: 56698
Efr3a
Name: EFR3 homolog A
Synonyms: A130089M23Rik, D030063F01Rik, C920006C10Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 76740
Homologene: 44222
Ncoa7
Name: nuclear receptor coactivator 7
Synonyms: 9030406N13Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 211329
VEGA: 10
Homologene: 65245
Ercc6
Name: excision repair cross-complementing rodent repair deficiency, complementation group 6
Synonyms: CS group B correcting gene, CSB, C130058G22Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 319955
VEGA: 14
HGNC: HGNC:3438
Homologene: 133552
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 34,201,405 bp
  • A to G, chromosome 1 at 136,159,649 bp
  • A to T, chromosome 1 at 150,603,814 bp
  • G to A, chromosome 1 at 171,174,465 bp
  • A to G, chromosome 1 at 173,729,015 bp
  • A to T, chromosome 1 at 173,875,619 bp
  • A to T, chromosome 4 at 41,116,686 bp
  • G to T, chromosome 4 at 63,964,762 bp
  • G to A, chromosome 4 at 83,020,755 bp
  • A to G, chromosome 4 at 108,588,714 bp
  • A to T, chromosome 4 at 115,327,559 bp
  • T to C, chromosome 5 at 34,917,695 bp
  • A to G, chromosome 5 at 52,845,330 bp
  • A to T, chromosome 5 at 67,342,119 bp
  • A to T, chromosome 5 at 72,715,883 bp
  • A to T, chromosome 5 at 72,974,659 bp
  • G to A, chromosome 5 at 88,642,952 bp
  • A to T, chromosome 5 at 137,465,232 bp
  • A to G, chromosome 5 at 139,342,647 bp
  • T to A, chromosome 6 at 66,539,810 bp
  • A to T, chromosome 7 at 3,717,188 bp
  • C to T, chromosome 7 at 33,519,905 bp
  • T to C, chromosome 7 at 44,611,553 bp
  • G to T, chromosome 7 at 139,635,146 bp
  • G to A, chromosome 8 at 16,058,707 bp
  • T to A, chromosome 8 at 70,716,804 bp
  • G to A, chromosome 8 at 85,518,052 bp
  • C to T, chromosome 8 at 105,955,715 bp
  • T to C, chromosome 8 at 110,711,620 bp
  • A to C, chromosome 9 at 54,401,585 bp
  • C to A, chromosome 9 at 71,725,883 bp
  • T to C, chromosome 9 at 83,548,489 bp
  • C to T, chromosome 9 at 86,773,545 bp
  • A to G, chromosome 9 at 106,693,461 bp
  • T to C, chromosome 9 at 114,417,058 bp
  • T to A, chromosome 10 at 20,233,462 bp
  • T to A, chromosome 10 at 20,987,077 bp
  • A to G, chromosome 10 at 30,689,800 bp
  • A to C, chromosome 10 at 76,272,562 bp
  • C to A, chromosome 11 at 99,780,408 bp
  • A to T, chromosome 11 at 103,360,541 bp
  • T to G, chromosome 12 at 8,987,312 bp
  • A to G, chromosome 12 at 86,256,872 bp
  • G to T, chromosome 12 at 98,680,101 bp
  • T to C, chromosome 13 at 15,725,559 bp
  • A to G, chromosome 13 at 64,225,647 bp
  • T to A, chromosome 13 at 95,635,655 bp
  • A to T, chromosome 13 at 100,041,532 bp
  • A to G, chromosome 14 at 32,526,404 bp
  • T to C, chromosome 14 at 34,571,802 bp
  • T to C, chromosome 14 at 53,876,057 bp
  • A to G, chromosome 15 at 10,346,438 bp
  • A to G, chromosome 15 at 65,819,778 bp
  • C to T, chromosome 15 at 81,627,314 bp
  • T to A, chromosome 15 at 102,991,110 bp
  • C to T, chromosome 16 at 20,548,680 bp
  • G to T, chromosome 16 at 49,173,006 bp
  • A to C, chromosome 16 at 57,570,937 bp
  • A to T, chromosome 16 at 59,186,013 bp
  • T to C, chromosome 17 at 22,559,051 bp
  • C to T, chromosome 17 at 32,415,919 bp
  • T to C, chromosome 17 at 36,951,008 bp
  • A to G, chromosome 17 at 56,062,573 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7327 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045420-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.