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Strain Name:
C57BL/6J-MtgxR7427Btlr/Mmmh
Stock Number:
045505-MU
Citation ID:
RRID:MMRRC_045505-MU
Other Names:
R7427 (G1)
Major Collection:

Strain Information

Kit
Name: KIT proto-oncogene receptor tyrosine kinase
Synonyms: Steel Factor Receptor, c-KIT, Dominant white spotting, belly-spot, Tr-kit, SOW3, SCO5, SCO1, Gsfsow3, Gsfsco5, Gsfsco1, CD117
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16590
HGNC: HGNC:6342
Homologene: 187
Slc12a1
Name: solute carrier family 12, member 1
Synonyms: Nkcc2, mBSC1, D630042G03Rik, urehr3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20495
Homologene: 286
Hlcs
Name: holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase)
Synonyms: 410I21.SP6, D16Jhu34
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 110948
VEGA: 16
HGNC: HGNC:4976
Homologene: 37302
Pcdh9
Name: protocadherin 9
Synonyms: C630029H24Rik, 1500001L12Rik, LOC382930, A730003J17Rik, C530050I23Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 211712
HGNC: HGNC:8661
Homologene: 10698
Omd
Name: osteomodulin
Synonyms: osteoadherin, SLRR2C, OSAD
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 27047
VEGA: 13
HGNC: HGNC:8134
Homologene: 3677
Srd5a3
Name: steroid 5 alpha-reductase 3
Synonyms: D730040M03Rik, 1110025P14Rik, Srd5a2l
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 57357
Homologene: 41385
Ccn2
Name: cellular communication network factor 2
Synonyms: Hcs24, hypertrophic chondrocyte-specific gene product 24, Fisp12, Ccn2, Ctgf
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14219
HGNC: HGNC:2500
Homologene: 1431
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 93,289,358 bp
  • A to T, chromosome 1 at 132,302,967 bp
  • C to T, chromosome 1 at 135,825,838 bp
  • T to A, chromosome 1 at 139,457,616 bp
  • A to C, chromosome 2 at 21,827,318 bp
  • C to T, chromosome 2 at 23,256,994 bp
  • C to A, chromosome 2 at 24,349,542 bp
  • T to A, chromosome 2 at 35,170,534 bp
  • T to G, chromosome 2 at 76,720,354 bp
  • T to A, chromosome 2 at 85,904,131 bp
  • A to T, chromosome 2 at 86,008,219 bp
  • G to T, chromosome 2 at 87,371,690 bp
  • T to C, chromosome 2 at 92,413,047 bp
  • C to A, chromosome 2 at 125,214,132 bp
  • A to G, chromosome 2 at 127,539,811 bp
  • G to A, chromosome 2 at 154,225,122 bp
  • T to A, chromosome 2 at 178,033,174 bp
  • G to A, chromosome 3 at 92,365,944 bp
  • G to T, chromosome 3 at 158,198,141 bp
  • A to T, chromosome 4 at 60,138,454 bp
  • T to C, chromosome 4 at 88,368,751 bp
  • A to T, chromosome 4 at 116,820,865 bp
  • A to G, chromosome 4 at 148,051,603 bp
  • T to C, chromosome 4 at 152,038,308 bp
  • T to C, chromosome 5 at 29,474,213 bp
  • G to A, chromosome 5 at 75,645,847 bp
  • A to G, chromosome 5 at 76,154,643 bp
  • A to G, chromosome 5 at 123,510,731 bp
  • A to C, chromosome 5 at 138,239,760 bp
  • A to T, chromosome 6 at 5,268,995 bp
  • C to T, chromosome 6 at 41,625,153 bp
  • A to T, chromosome 6 at 90,346,362 bp
  • T to G, chromosome 6 at 142,003,508 bp
  • A to T, chromosome 7 at 4,716,210 bp
  • C to T, chromosome 7 at 18,611,983 bp
  • C to T, chromosome 7 at 25,338,371 bp
  • T to C, chromosome 7 at 26,371,435 bp
  • T to C, chromosome 7 at 85,411,259 bp
  • A to T, chromosome 7 at 102,135,001 bp
  • A to G, chromosome 7 at 102,640,326 bp
  • A to G, chromosome 7 at 128,679,805 bp
  • A to G, chromosome 8 at 16,023,850 bp
  • A to C, chromosome 8 at 40,378,914 bp
  • A to G, chromosome 8 at 107,170,416 bp
  • T to C, chromosome 9 at 71,505,183 bp
  • T to C, chromosome 9 at 87,019,756 bp
  • T to C, chromosome 9 at 109,108,168 bp
  • A to T, chromosome 9 at 123,810,240 bp
  • T to C, chromosome 10 at 5,273,718 bp
  • T to C, chromosome 10 at 14,133,741 bp
  • T to G, chromosome 10 at 24,597,499 bp
  • T to A, chromosome 10 at 53,089,414 bp
  • T to A, chromosome 10 at 69,248,824 bp
  • T to C, chromosome 10 at 80,131,304 bp
  • C to A, chromosome 10 at 84,896,012 bp
  • T to C, chromosome 10 at 89,498,405 bp
  • A to G, chromosome 11 at 58,489,780 bp
  • T to C, chromosome 11 at 65,955,219 bp
  • A to G, chromosome 11 at 70,458,804 bp
  • T to C, chromosome 12 at 17,278,545 bp
  • T to C, chromosome 12 at 36,381,538 bp
  • G to T, chromosome 12 at 87,286,995 bp
  • A to T, chromosome 13 at 49,592,269 bp
  • A to G, chromosome 13 at 65,153,052 bp
  • A to G, chromosome 13 at 112,488,560 bp
  • A to G, chromosome 14 at 29,064,275 bp
  • T to C, chromosome 14 at 31,159,259 bp
  • C to T, chromosome 14 at 31,240,413 bp
  • T to A, chromosome 14 at 49,298,898 bp
  • T to C, chromosome 14 at 64,788,460 bp
  • G to T, chromosome 14 at 93,887,111 bp
  • T to A, chromosome 15 at 76,594,385 bp
  • A to G, chromosome 16 at 94,267,899 bp
  • A to T, chromosome 17 at 18,326,410 bp
  • A to T, chromosome 17 at 25,841,609 bp
  • T to A, chromosome 17 at 35,930,133 bp
  • T to C, chromosome 17 at 37,383,299 bp
  • A to T, chromosome 18 at 80,089,652 bp
  • T to C, chromosome 19 at 46,137,424 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7427 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045505-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.