Strain Name:
C57BL/6J-MtgxR7442Btlr/Mmmh
Stock Number:
045518-MU
Citation ID:
RRID:MMRRC_045518-MU
Other Names:
R7442 (G1)
Major Collection:

Strain Information

Enc1
Name: ectodermal-neural cortex 1
Synonyms: PIG10, Nrpb
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 13803
VEGA: 13
HGNC: HGNC:3345
Homologene: 2694
Ptprk
Name: protein tyrosine phosphatase receptor type K
Synonyms: PTPk, RPTPkappa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19272
VEGA: 10
HGNC: HGNC:9674
Homologene: 55693
Etv5
Name: ets variant 5
Synonyms: 8430401F14Rik, erm, 1110005E01Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 104156
HGNC: HGNC:3494
Homologene: 3276
Col18a1
Name: collagen, type XVIII, alpha 1
Synonyms: endostatin
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12822
HGNC: HGNC:2195
Homologene: 7673
Slc44a2
Name: solute carrier family 44, member 2
Synonyms: 1110028E10Rik, CTL2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 68682
VEGA: 9
Homologene: 10711
Igf1r
Name: insulin-like growth factor I receptor
Synonyms: CD221, IGF-1R, line 186, hyft, A330103N21Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16001
HGNC: HGNC:5465
Homologene: 30997
Zfhx3
Name: zinc finger homeobox 3
Synonyms: WBP9, A230102L03Rik, Atbf1, Sci
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11906
HGNC: HGNC:777
Homologene: 21366
Xpo4
Name: exportin 4
Synonyms: B430309A01Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 57258
Homologene: 10733
Ints9
Name: integrator complex subunit 9
Synonyms: D14Ertd231e
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 210925
VEGA: 14
Homologene: 10096
Srrm2
Name: serine/arginine repetitive matrix 2
Synonyms: 5033413A03Rik, SRm300
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 75956
St7l
Name: suppression of tumorigenicity 7-like
Synonyms: St7r
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229681
Homologene: 14910
Atp5f1a
Name: ATP synthase F1 subunit alpha
Synonyms: Atpm, D18Ertd206e, Mom2, Atp5a1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 11946
HGNC: HGNC:823
Homologene: 2985
Bod1l
Name: biorientation of chromosomes in cell division 1-like
Synonyms: A230054D04Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 665775
Homologene: 45109
Treml1
Name: triggering receptor expressed on myeloid cells-like 1
Synonyms: TLT-1, 5430401J17Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 71326
VEGA: 17
Homologene: 52257
Mia3
Name: MIA SH3 domain ER export factor 3
Synonyms: LOC385255, Tango, A930039G15Rik, B230399H06Rik, 9130229H14Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 338366
Homologene: 85165
Top3a
Name: topoisomerase (DNA) III alpha
Synonyms: Top IIIa
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 21975
Homologene: 3394
Zswim1
Name: zinc finger SWIM-type containing 1
Synonyms: 2410003H12Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71971
Homologene: 12429
Slain1
Name: SLAIN motif family, member 1
Synonyms: 9630044O09Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105439
Homologene: 16943
Styxl2
Name: serine/threonine/tyrosine interacting like 2
Synonyms: C130085G02Rik, Dusp27
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 240892
Homologene: 18949
Tmco3
Name: transmembrane and coiled-coil domains 3
Synonyms: B230339H12Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234076
Homologene: 32376
Tmem178
Name: transmembrane protein 178
Synonyms: 2810417M05Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 68027
VEGA: 17
Homologene: 12226
Cd68
Name: CD68 antigen
Synonyms: macrosialin, gp110, Scard1, Lamp4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12514
HGNC: HGNC:1693
Homologene: 955
Anapc4
Name: anaphase promoting complex subunit 4
Synonyms: 2610306D21Rik, APC4, D5Ertd249e
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 52206
Homologene: 40873
Fbn1
Name: fibrillin 1
Synonyms: Fib-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14118
HGNC: HGNC:3603
Homologene: 30958
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Myo16
Name: myosin XVI
Synonyms: C230040D10Rik, Nyap3, BM140241
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244281
Homologene: 34710
Ptprd
Name: protein tyrosine phosphatase receptor type D
Synonyms: 3000002J10Rik, B230219D21Rik, 1110002J03Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 19266
HGNC: HGNC:9668
Lama3
Name: laminin, alpha 3
Synonyms: nicein, 150kDa, [a]3B
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 16774
HGNC: HGNC:6483
Homologene: 18279
Ephb6
Name: Eph receptor B6
Synonyms: Mep, Cekl
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13848
HGNC: HGNC:3396
Homologene: 20940
Or2r3
Name: olfactory receptor family 2 subfamily R member 3
Synonyms: GA_x6K02T2P3E9-5088893-5089834, MOR257-1, Olfr457
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 258989
Homologene: 133701
Xpc
Name: xeroderma pigmentosum, complementation group C
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 22591
Homologene: 3401
Arhgap5
Name: Rho GTPase activating protein 5
Synonyms: p190-B, p190B
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 11855
VEGA: 12
HGNC: HGNC:675
Homologene: 907
Ifi207
Name: interferon activated gene 207
Synonyms: AI607873, Pyhin-A
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226691
HGNC: HGNC:5395
Homologene: 115929
Ccdc168
Name: coiled-coil domain containing 168
Synonyms: Gm8251
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 102636082
VEGA: 1
Homologene: 141149
Exoc3l
Name: exocyst complex component 3-like
Synonyms: C730015A04Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 277978
Homologene: 18629
Aox1
Name: aldehyde oxidase 1
Synonyms: retinal oxidase, Aox-1, Aox-2, Aox2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 11761
HGNC: HGNC:553
Homologene: 68165
Edem1
Name: ER degradation enhancer, mannosidase alpha-like 1
Synonyms: A130059K23Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 192193
Homologene: 33836
Or56a4
Name: olfactory receptor family 56 subfamily A member 4
Synonyms: GA_x6K02T2PBJ9-7786441-7785503, MOR40-8P, Olfr684
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 244187
Homologene: 133596
Cacna1b
Name: calcium channel, voltage-dependent, N type, alpha 1B subunit
Synonyms: Cchn1a, alpha(1B), Cav2.2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12287
HGNC: HGNC:1389
Homologene: 20184
Mrps21
Name: mitochondrial ribosomal protein S21
Synonyms: 1810031B19Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 66292
Homologene: 45365
Adgre4
Name: adhesion G protein-coupled receptor E4
Synonyms: EGF-TM7, D17Ertd479e, FIRE, Gpr127, Emr4
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 52614
VEGA: 17
Homologene: 64640
Mdm1
Name: MDM1 nuclear protein
Synonyms: Mdm-1, transformed mouse 3T3 cell double minute 1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17245
VEGA: 10
Homologene: 9692
Tmem229a
Name: transmembrane protein 229A
Synonyms: 6332401O19Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 319832
Homologene: 85312
Mybpc1
Name: myosin binding protein C, slow-type
Synonyms: Slow-type C-protein, 8030451F13Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 109272
HGNC: HGNC:7549
Homologene: 1846
Trim42
Name: tripartite motif-containing 42
Synonyms: 4930486B16Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 78911
Homologene: 12712
Adam9
Name: ADAM metallopeptidase domain 9
Synonyms: MDC9, Mltng, MDC9, Mltng
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11502
HGNC: HGNC:216
Homologene: 20824
Dsc3
Name: desmocollin 3
Synonyms: 5430426I24Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 13507
VEGA: 18
HGNC: HGNC:3037
Homologene: 1462
Bst1
Name: bone marrow stromal cell antigen 1
Synonyms: 114/A10, Ly65, Bp3, Bsta1, CD157
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12182
HGNC: HGNC:1118
Homologene: 3198
A430033K04Rik
Name: RIKEN cDNA A430033K04 gene
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 243308
Homologene: 65621
Acap3
Name: ArfGAP with coiled-coil, ankyrin repeat and PH domains 3
Synonyms: Kiaa1716-hp, Centb5
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 140500
Homologene: 23708
Foxe3
Name: forkhead box E3
Synonyms: FREAC8, rct
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 30923
HGNC: HGNC:3808
Homologene: 32145
Vmn1r86
Name: vomeronasal 1 receptor 86
Synonyms: Gm10301
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 100312473
Homologene: 74345
Vmn2r96
Name: vomeronasal 2, receptor 96
Synonyms: EG433070
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 433070
1700011L22Rik
Name: RIKEN cDNA 1700011L22 gene
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 67687
Homologene: 78034
Setdb2
Name: SET domain, bifurcated 2
Synonyms: LOC239122, KMT1F
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 239122
Homologene: 12903
Obox1
Name: oocyte specific homeobox 1
Synonyms: 7420700M11Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 71468
Homologene: 44937
Or5ar1
Name: olfactory receptor family 5 subfamily AR member 1
Synonyms: GA_x6K02T2Q125-47320309-47319377, MOR180-1, Olfr1019
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 259017
Homologene: 17464
Dyrk2
Name: dual-specificity tyrosine phosphorylation regulated kinase 2
Synonyms: 1810038L18Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 69181
VEGA: 10
HGNC: HGNC:3093
Homologene: 48437
Lrtm2
Name: leucine-rich repeats and transmembrane domains 2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 211187
Homologene: 65244
Tktl2
Name: transketolase-like 2
Synonyms: 4933401I19Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 74419
Homologene: 69456
Slc10a2
Name: solute carrier family 10, member 2
Synonyms: ASBT, 9130221J18Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 20494
Homologene: 390
Asah1
Name: N-acylsphingosine amidohydrolase 1
Synonyms: acid ceramidase, 2310081N20Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11886
HGNC: HGNC:735
Homologene: 10504
Caps2
Name: calcyphosphine 2
Synonyms: D630005B03Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 353025
Homologene: 72278
Pkd2l1
Name: polycystic kidney disease 2-like 1
Synonyms: polycystin-L, Pkdl, PCL, TRPP3, PKD2L
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 329064
HGNC: HGNC:9011
Homologene: 22946
Or12d2
Name: olfactory receptor family 12 subfamily D member 2
Synonyms: GA_x6K02T2PSCP-1775063-1774137, MOR250-4, Olfr102
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 258218
Homologene: 133728
Ptpn18
Name: protein tyrosine phosphatase, non-receptor type 18
Synonyms: HSCF, FLP1, Ptpk1, PTP-K1, PTP-HSCF
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19253
HGNC: HGNC:9649
Homologene: 74971
Mylk2
Name: myosin, light polypeptide kinase 2, skeletal muscle
Synonyms: 9830004H17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228785
Homologene: 13223
Nudt18
Name: nudix hydrolase 18
Synonyms: nudix (nucleoside diphosphate linked moiety X)-type motif 18
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 213484
VEGA: 14
Homologene: 32604
Ankdd1b
Name: ankyrin repeat and death domain containing 1B
Synonyms: 9330128J19Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 271144
Homologene: 19566
Slco4a1
Name: solute carrier organic anion transporter family, member 4a1
Synonyms: OATP-E, Slc21a12
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 108115
Homologene: 9482
Hoxd1
Name: homeobox D1
Synonyms: Hox-4.9
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 15429
HGNC: HGNC:5132
Homologene: 7772
Sun3
Name: Sad1 and UNC84 domain containing 3
Synonyms: D630047F21Rik, Sunc1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 194974
Homologene: 51273
Srp54c
Name: signal recognition particle 54C
Synonyms: E330029E12Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 100101806
VEGA: 12
Homologene: 129700
Gm49380
Name: predicted gene, 49380
Type: Gene
Species: Mouse
Chromosome: 9
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 34,462,750 bp
  • A to G, chromosome 1 at 44,058,708 bp
  • T to C, chromosome 1 at 58,082,013 bp
  • T to C, chromosome 1 at 166,101,015 bp
  • C to T, chromosome 1 at 173,727,431 bp
  • T to C, chromosome 1 at 183,358,876 bp
  • A to C, chromosome 2 at 24,607,501 bp
  • A to T, chromosome 2 at 74,763,559 bp
  • A to G, chromosome 2 at 76,743,006 bp
  • A to T, chromosome 2 at 85,841,752 bp
  • C to A, chromosome 2 at 125,403,212 bp
  • T to G, chromosome 2 at 152,911,426 bp
  • A to G, chromosome 2 at 164,825,790 bp
  • T to C, chromosome 2 at 180,474,126 bp
  • T to C, chromosome 3 at 95,862,816 bp
  • A to G, chromosome 3 at 104,889,329 bp
  • A to T, chromosome 4 at 76,059,821 bp
  • A to G, chromosome 4 at 114,925,293 bp
  • T to C, chromosome 4 at 155,905,621 bp
  • G to A, chromosome 5 at 41,807,179 bp
  • T to C, chromosome 5 at 43,821,742 bp
  • T to C, chromosome 5 at 52,857,201 bp
  • T to C, chromosome 5 at 138,647,247 bp
  • T to C, chromosome 5 at 146,292,769 bp
  • C to A, chromosome 6 at 24,955,690 bp
  • T to G, chromosome 6 at 41,618,047 bp
  • A to T, chromosome 6 at 42,471,500 bp
  • A to G, chromosome 6 at 91,504,649 bp
  • T to A, chromosome 6 at 108,851,305 bp
  • C to T, chromosome 6 at 119,317,431 bp
  • A to T, chromosome 7 at 13,102,056 bp
  • G to T, chromosome 7 at 15,555,566 bp
  • G to A, chromosome 7 at 68,173,278 bp
  • T to C, chromosome 7 at 105,157,082 bp
  • A to T, chromosome 8 at 5,089,086 bp
  • G to A, chromosome 8 at 10,272,537 bp
  • T to A, chromosome 8 at 13,320,781 bp
  • A to T, chromosome 8 at 24,967,207 bp
  • T to C, chromosome 8 at 41,343,565 bp
  • A to G, chromosome 8 at 66,512,909 bp
  • A to G, chromosome 8 at 79,220,290 bp
  • A to G, chromosome 8 at 105,292,926 bp
  • A to G, chromosome 8 at 108,792,836 bp
  • C to T, chromosome 9 at 21,345,523 bp
  • T to A, chromosome 9 at 44,112,412 bp
  • C to T, chromosome 9 at 97,362,945 bp
  • G to A, chromosome 10 at 28,574,819 bp
  • A to T, chromosome 10 at 77,096,238 bp
  • A to C, chromosome 10 at 88,526,293 bp
  • G to T, chromosome 10 at 112,208,354 bp
  • T to C, chromosome 10 at 118,146,685 bp
  • T to C, chromosome 10 at 118,859,881 bp
  • T to G, chromosome 11 at 9,031,445 bp
  • C to T, chromosome 11 at 60,753,918 bp
  • T to C, chromosome 11 at 69,665,928 bp
  • A to G, chromosome 12 at 52,516,956 bp
  • T to A, chromosome 12 at 55,255,562 bp
  • T to G, chromosome 13 at 96,424,760 bp
  • A to T, chromosome 13 at 97,246,740 bp
  • A to G, chromosome 14 at 47,714,640 bp
  • A to G, chromosome 14 at 57,630,223 bp
  • A to G, chromosome 14 at 59,419,251 bp
  • C to T, chromosome 14 at 64,995,064 bp
  • A to C, chromosome 14 at 70,579,358 bp
  • A to G, chromosome 14 at 103,685,714 bp
  • G to T, chromosome 16 at 22,436,059 bp
  • T to C, chromosome 17 at 18,573,400 bp
  • T to C, chromosome 17 at 23,820,117 bp
  • T to A, chromosome 17 at 37,313,925 bp
  • C to A, chromosome 17 at 48,366,691 bp
  • T to A, chromosome 17 at 55,852,340 bp
  • T to A, chromosome 17 at 80,944,756 bp
  • G to A, chromosome 18 at 12,472,181 bp
  • T to C, chromosome 18 at 19,981,156 bp
  • G to A, chromosome 18 at 77,779,120 bp
  • T to C, chromosome 19 at 44,157,229 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7442 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045518-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.