Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR7489Btlr/Mmmh
Stock Number:
045563-MU
Citation ID:
RRID:MMRRC_045563-MU
Other Names:
R7489 (G1)
Major Collection:

Strain Information

Kl
Name: klotho
Synonyms: alpha-kl
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16591
HGNC: HGNC:6344
Homologene: 68415
Gli2
Name: GLI-Kruppel family member GLI2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14633
HGNC: HGNC:4318
Homologene: 12725
Fancd2
Name: Fanconi anemia, complementation group D2
Synonyms: 2410150O07Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 211651
HGNC: HGNC:3585
Homologene: 13212
Dip2c
Name: disco interacting protein 2 homolog C
Synonyms: 2900024P20Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 208440
Homologene: 40996
Abtb3
Name: ankyrin repeat and BTB domain containing 3
Synonyms: 6330404E16Rik, Btbd11
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 74007
Homologene: 72536
Trip10
Name: thyroid hormone receptor interactor 10
Synonyms: Cip4
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 106628
VEGA: 17
Homologene: 99728
Atp2a2
Name: ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
Synonyms: sarco/endoplasmic reticulum Ca2+-ATPase 2, SERCA2, 9530097L16Rik, D5Wsu150e, SERCA2B, Serca2a
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 11938
HGNC: HGNC:812
Homologene: 80167
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 25,547,505 bp
  • T to A, chromosome 1 at 88,379,759 bp
  • A to G, chromosome 1 at 118,838,175 bp
  • T to C, chromosome 1 at 163,949,176 bp
  • T to A, chromosome 2 at 5,001,301 bp
  • A to C, chromosome 2 at 67,525,560 bp
  • T to A, chromosome 2 at 90,086,360 bp
  • A to C, chromosome 2 at 153,944,004 bp
  • A to T, chromosome 3 at 41,629,597 bp
  • G to A, chromosome 3 at 92,680,647 bp
  • A to G, chromosome 4 at 61,303,888 bp
  • T to C, chromosome 4 at 111,526,658 bp
  • C to A, chromosome 4 at 111,883,916 bp
  • G to A, chromosome 4 at 152,373,468 bp
  • C to T, chromosome 5 at 4,004,933 bp
  • T to C, chromosome 5 at 45,500,506 bp
  • T to C, chromosome 5 at 108,797,656 bp
  • T to C, chromosome 5 at 114,879,625 bp
  • T to C, chromosome 5 at 122,467,767 bp
  • C to A, chromosome 5 at 137,322,820 bp
  • A to T, chromosome 5 at 147,306,672 bp
  • A to T, chromosome 5 at 150,952,996 bp
  • A to G, chromosome 6 at 39,388,105 bp
  • T to C, chromosome 6 at 48,473,713 bp
  • C to T, chromosome 6 at 57,843,892 bp
  • T to C, chromosome 6 at 113,564,304 bp
  • A to T, chromosome 6 at 121,226,021 bp
  • A to G, chromosome 7 at 29,366,702 bp
  • A to T, chromosome 7 at 96,837,314 bp
  • C to T, chromosome 7 at 99,268,061 bp
  • G to T, chromosome 7 at 105,567,480 bp
  • A to C, chromosome 8 at 71,684,292 bp
  • C to T, chromosome 8 at 85,767,001 bp
  • A to T, chromosome 8 at 93,178,131 bp
  • A to G, chromosome 8 at 104,929,780 bp
  • A to T, chromosome 9 at 7,561,387 bp
  • T to A, chromosome 9 at 8,656,544 bp
  • A to G, chromosome 9 at 9,006,358 bp
  • T to A, chromosome 9 at 71,561,038 bp
  • A to T, chromosome 9 at 106,241,634 bp
  • C to A, chromosome 9 at 106,627,457 bp
  • C to T, chromosome 9 at 108,986,254 bp
  • T to C, chromosome 10 at 20,963,750 bp
  • T to A, chromosome 10 at 24,904,356 bp
  • T to A, chromosome 10 at 85,627,215 bp
  • A to T, chromosome 10 at 129,874,682 bp
  • G to C, chromosome 11 at 46,407,441 bp
  • C to T, chromosome 11 at 83,990,825 bp
  • T to C, chromosome 11 at 90,656,359 bp
  • C to T, chromosome 12 at 79,300,585 bp
  • A to G, chromosome 12 at 112,067,637 bp
  • T to C, chromosome 13 at 9,533,312 bp
  • T to A, chromosome 13 at 93,091,838 bp
  • C to A, chromosome 13 at 109,116,767 bp
  • A to T, chromosome 14 at 44,170,299 bp
  • T to A, chromosome 15 at 12,152,982 bp
  • A to C, chromosome 15 at 101,793,859 bp
  • A to T, chromosome 16 at 88,627,234 bp
  • A to T, chromosome 17 at 35,162,354 bp
  • A to C, chromosome 17 at 36,989,471 bp
  • A to T, chromosome 17 at 55,866,158 bp
  • A to T, chromosome 17 at 57,250,966 bp
  • T to A, chromosome 18 at 24,282,157 bp
  • T to C, chromosome 18 at 38,281,789 bp
  • T to A, chromosome 18 at 46,560,517 bp
  • A to G, chromosome 19 at 9,997,046 bp
  • T to C, chromosome 19 at 11,302,031 bp
  • A to T, chromosome 19 at 18,642,242 bp
  • G to T, chromosome 19 at 18,642,243 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7489 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045563-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.