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Strain Name:
C57BL/6J-MtgxR7508Btlr/Mmmh
Stock Number:
045581-MU
Citation ID:
RRID:MMRRC_045581-MU
Other Names:
R7508 (G1)
Major Collection:

Strain Information

Pik3cd
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta
Synonyms: p110delta, 2410099E07Rik, 2610208K16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18707
HGNC: HGNC:8977
Homologene: 3686
Clasp1
Name: CLIP associating protein 1
Synonyms: 5730583A19Rik, 1700030C23Rik, mCLASP1, CLASP1alpha, CLASP1, B130045P17Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 76707
Homologene: 41024
Taok1
Name: TAO kinase 1
Synonyms: D130018F14Rik, 2810468K05Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216965
Homologene: 27041
Rbm25
Name: RNA binding motif protein 25
Synonyms: 2610015J01Rik, A130095G20Rik, 2600011C06Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 67039
Helb
Name: helicase (DNA) B
Synonyms: D10Ertd664e
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 117599
Homologene: 50463
Ube3a
Name: ubiquitin protein ligase E3A
Synonyms: Hpve6a, E6-AP ubiquitin protein ligase, A130086L21Rik, 5830462N02Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22215
Homologene: 7988
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 58,017,795 bp
  • A to T, chromosome 1 at 69,887,520 bp
  • A to G, chromosome 1 at 71,597,516 bp
  • A to G, chromosome 1 at 74,687,259 bp
  • T to A, chromosome 1 at 118,545,434 bp
  • A to G, chromosome 1 at 135,278,858 bp
  • T to A, chromosome 2 at 72,205,733 bp
  • A to G, chromosome 2 at 86,121,938 bp
  • T to A, chromosome 2 at 173,207,811 bp
  • A to G, chromosome 3 at 57,294,755 bp
  • T to C, chromosome 3 at 87,083,439 bp
  • A to G, chromosome 3 at 122,818,030 bp
  • G to A, chromosome 4 at 41,614,323 bp
  • T to A, chromosome 4 at 51,229,256 bp
  • A to G, chromosome 4 at 88,437,478 bp
  • A to G, chromosome 4 at 149,654,583 bp
  • C to G, chromosome 5 at 31,473,539 bp
  • C to T, chromosome 5 at 50,016,867 bp
  • C to T, chromosome 5 at 142,718,506 bp
  • T to A, chromosome 6 at 48,466,699 bp
  • A to T, chromosome 6 at 58,987,284 bp
  • A to G, chromosome 6 at 61,570,723 bp
  • A to T, chromosome 6 at 130,230,091 bp
  • C to T, chromosome 7 at 7,467,374 bp
  • T to A, chromosome 7 at 59,303,689 bp
  • T to C, chromosome 8 at 71,354,801 bp
  • T to C, chromosome 8 at 84,704,300 bp
  • T to A, chromosome 8 at 105,363,253 bp
  • T to A, chromosome 9 at 108,836,622 bp
  • A to G, chromosome 9 at 114,731,240 bp
  • G to A, chromosome 10 at 61,300,155 bp
  • A to G, chromosome 10 at 62,971,993 bp
  • C to T, chromosome 10 at 116,353,991 bp
  • T to C, chromosome 10 at 120,105,283 bp
  • A to C, chromosome 10 at 123,023,939 bp
  • A to T, chromosome 11 at 20,306,487 bp
  • T to C, chromosome 11 at 50,145,120 bp
  • A to T, chromosome 11 at 68,922,423 bp
  • T to A, chromosome 11 at 77,545,326 bp
  • T to C, chromosome 12 at 54,180,628 bp
  • T to C, chromosome 12 at 83,672,877 bp
  • T to C, chromosome 12 at 87,494,842 bp
  • A to G, chromosome 12 at 108,882,875 bp
  • C to T, chromosome 12 at 112,774,405 bp
  • G to A, chromosome 12 at 114,788,442 bp
  • T to A, chromosome 13 at 21,638,609 bp
  • T to A, chromosome 13 at 40,887,681 bp
  • T to C, chromosome 13 at 65,279,273 bp
  • C to T, chromosome 13 at 75,878,857 bp
  • C to T, chromosome 14 at 54,212,714 bp
  • A to G, chromosome 14 at 56,100,541 bp
  • A to C, chromosome 14 at 66,637,935 bp
  • T to G, chromosome 15 at 21,583,765 bp
  • G to A, chromosome 15 at 99,722,086 bp
  • C to G, chromosome 17 at 26,438,746 bp
  • A to G, chromosome 17 at 33,003,782 bp
  • G to A, chromosome 17 at 47,672,280 bp
  • A to G, chromosome 17 at 47,734,186 bp
  • T to C, chromosome 18 at 57,904,393 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7508 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045581-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.