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Strain Name:
C57BL/6J-MtgxR7529Btlr/Mmmh
Stock Number:
045601-MU
Citation ID:
RRID:MMRRC_045601-MU
Other Names:
R7529 (G1)
Major Collection:

Strain Information

Prrx1
Name: paired related homeobox 1
Synonyms: K-2, Prx1, mHox, Pmx1, mHox, A230024N07Rik, MHox1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18933
HGNC: HGNC:9142
Homologene: 7896
Dtnbp1
Name: dystrobrevin binding protein 1
Synonyms: dysbindin, 5430437B18Rik, sdy, Bloc1s8
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 94245
VEGA: 13
Homologene: 12037
Med1
Name: mediator complex subunit 1
Synonyms: TRAP 220, TRAP220, CRSP210, DRIP205, Pparbp, l11Jus15, PBP
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 19014
HGNC: HGNC:9234
Homologene: 21002
Ggta1
Name: glycoprotein galactosyltransferase alpha 1, 3
Synonyms: GALT, alpha Gal, Gal, glycoprotein alpha galactosyl transferase 1, alpha3GalT, Ggta-1, Ggta
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14594
HGNC: HGNC:4253
Homologene: 7730
Rnmt
Name: RNA (guanine-7-) methyltransferase
Synonyms: 2610002P10Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 67897
Homologene: 2816
Luzp1
Name: leucine zipper protein 1
Synonyms: Luzp, 2700072H04Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269593
Homologene: 11545
Ints1
Name: integrator complex subunit 1
Synonyms: 1110015K06Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 68510
Homologene: 53111
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 9,546,192 bp
  • T to C, chromosome 1 at 74,926,610 bp
  • A to G, chromosome 1 at 94,009,317 bp
  • A to C, chromosome 1 at 143,646,536 bp
  • A to G, chromosome 1 at 155,861,923 bp
  • T to C, chromosome 1 at 163,253,964 bp
  • A to T, chromosome 1 at 163,943,869 bp
  • T to C, chromosome 1 at 194,643,871 bp
  • A to G, chromosome 2 at 35,414,244 bp
  • C to A, chromosome 2 at 75,832,352 bp
  • T to C, chromosome 2 at 118,710,234 bp
  • T to C, chromosome 2 at 152,915,704 bp
  • T to G, chromosome 2 at 165,352,808 bp
  • T to C, chromosome 2 at 167,507,094 bp
  • C to T, chromosome 2 at 168,894,072 bp
  • C to T, chromosome 2 at 180,058,157 bp
  • G to A, chromosome 3 at 83,354,398 bp
  • G to A, chromosome 3 at 116,027,406 bp
  • A to T, chromosome 3 at 159,873,007 bp
  • C to A, chromosome 4 at 117,213,741 bp
  • T to A, chromosome 4 at 130,384,482 bp
  • T to C, chromosome 4 at 132,334,563 bp
  • G to T, chromosome 4 at 136,540,932 bp
  • G to C, chromosome 4 at 139,422,417 bp
  • T to C, chromosome 4 at 143,702,674 bp
  • A to G, chromosome 5 at 131,306,380 bp
  • G to A, chromosome 5 at 139,767,726 bp
  • A to T, chromosome 6 at 38,630,331 bp
  • A to G, chromosome 6 at 42,675,355 bp
  • T to A, chromosome 6 at 67,490,736 bp
  • G to T, chromosome 6 at 72,223,841 bp
  • G to A, chromosome 6 at 119,270,766 bp
  • A to T, chromosome 6 at 146,194,598 bp
  • A to C, chromosome 7 at 27,189,453 bp
  • A to T, chromosome 7 at 87,395,768 bp
  • T to A, chromosome 7 at 128,237,164 bp
  • A to G, chromosome 7 at 142,229,692 bp
  • A to T, chromosome 8 at 25,724,206 bp
  • C to G, chromosome 9 at 21,007,679 bp
  • T to C, chromosome 9 at 25,108,897 bp
  • T to C, chromosome 9 at 44,138,895 bp
  • A to C, chromosome 9 at 71,631,758 bp
  • T to A, chromosome 10 at 5,424,382 bp
  • A to T, chromosome 10 at 24,798,174 bp
  • A to T, chromosome 10 at 58,612,008 bp
  • T to C, chromosome 11 at 7,139,157 bp
  • A to G, chromosome 11 at 49,112,859 bp
  • G to T, chromosome 11 at 68,954,022 bp
  • T to C, chromosome 11 at 85,840,901 bp
  • G to C, chromosome 11 at 98,155,965 bp
  • A to G, chromosome 11 at 118,049,866 bp
  • A to G, chromosome 12 at 44,283,180 bp
  • A to G, chromosome 12 at 51,371,604 bp
  • A to G, chromosome 12 at 84,131,343 bp
  • A to T, chromosome 12 at 102,264,448 bp
  • A to G, chromosome 12 at 113,736,334 bp
  • T to A, chromosome 13 at 27,560,528 bp
  • T to C, chromosome 13 at 38,025,755 bp
  • A to G, chromosome 13 at 44,931,070 bp
  • A to G, chromosome 13 at 49,100,981 bp
  • A to T, chromosome 13 at 93,097,434 bp
  • T to A, chromosome 14 at 32,560,729 bp
  • T to C, chromosome 14 at 47,416,419 bp
  • G to T, chromosome 14 at 63,819,161 bp
  • T to A, chromosome 14 at 64,109,588 bp
  • A to G, chromosome 15 at 4,949,009 bp
  • G to T, chromosome 15 at 66,694,768 bp
  • T to C, chromosome 16 at 32,298,914 bp
  • T to A, chromosome 17 at 3,671,775 bp
  • A to T, chromosome 17 at 21,880,352 bp
  • T to A, chromosome 17 at 22,007,159 bp
  • A to G, chromosome 17 at 24,597,948 bp
  • G to T, chromosome 17 at 34,824,656 bp
  • A to T, chromosome 17 at 35,619,231 bp
  • G to T, chromosome 18 at 37,474,473 bp
  • A to T, chromosome 18 at 44,346,173 bp
  • A to G, chromosome 18 at 61,198,358 bp
  • A to C, chromosome 18 at 61,821,968 bp
  • A to G, chromosome 18 at 68,311,655 bp
  • A to G, chromosome 19 at 12,298,722 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7529 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045601-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.