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Strain Name:
C57BL/6J-MtgxR7539Btlr/Mmmh
Stock Number:
045611-MU
Citation ID:
RRID:MMRRC_045611-MU
Other Names:
R7539 (G1)
Major Collection:

Strain Information

Bet1l
Name: Bet1 golgi vesicular membrane trafficking protein like
Synonyms: 2610021K23Rik, golgi SNARE, Gs15
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 54399
Homologene: 10293
Utp20
Name: UTP20 small subunit processome component
Synonyms: mDRIM, DRIM, 3830408P06Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70683
VEGA: 10
Homologene: 38373
Pik3c2a
Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha
Synonyms: PI3KC2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18704
HGNC: HGNC:8971
Homologene: 20581
Dock9
Name: dedicator of cytokinesis 9
Synonyms: B230309H04Rik, D14Wsu89e, Zizimin1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105445
Homologene: 41026
Birc6
Name: baculoviral IAP repeat-containing 6
Synonyms: apollon, Bruce, A430032G04Rik, D630005A10Rik, A430040A19Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 12211
Homologene: 7248
Dop1a
Name: DOP1 leucine zipper like protein A
Synonyms: B130005I07Rik, D9Ertd809e, Dopey1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 320615
Homologene: 26645
Wasl
Name: WASP like actin nucleation promoting factor
Synonyms: N-WASP, 3110031I02Rik, 2900021I12Rik, Wiskott-Aldrich syndrome-like (human)
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 73178
Homologene: 135996
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 52,071,709 bp
  • T to G, chromosome 1 at 139,248,229 bp
  • T to C, chromosome 2 at 36,927,209 bp
  • T to C, chromosome 2 at 58,035,230 bp
  • T to C, chromosome 2 at 111,664,433 bp
  • A to T, chromosome 3 at 154,962,031 bp
  • C to G, chromosome 4 at 20,049,833 bp
  • C to T, chromosome 4 at 101,020,696 bp
  • T to A, chromosome 4 at 139,021,699 bp
  • A to T, chromosome 5 at 41,817,860 bp
  • G to C, chromosome 5 at 123,178,192 bp
  • A to T, chromosome 6 at 24,619,198 bp
  • T to C, chromosome 6 at 42,956,103 bp
  • A to C, chromosome 6 at 88,849,329 bp
  • C to A, chromosome 6 at 90,662,891 bp
  • T to C, chromosome 7 at 4,470,037 bp
  • T to C, chromosome 7 at 6,708,168 bp
  • C to A, chromosome 7 at 12,890,552 bp
  • T to C, chromosome 7 at 76,425,929 bp
  • T to A, chromosome 7 at 86,725,909 bp
  • T to C, chromosome 7 at 103,220,066 bp
  • A to G, chromosome 7 at 103,328,494 bp
  • T to C, chromosome 7 at 104,059,263 bp
  • T to C, chromosome 7 at 105,720,912 bp
  • T to C, chromosome 7 at 116,340,096 bp
  • T to C, chromosome 7 at 140,854,544 bp
  • T to C, chromosome 8 at 10,361,095 bp
  • C to A, chromosome 8 at 94,870,779 bp
  • A to T, chromosome 9 at 20,101,619 bp
  • T to G, chromosome 9 at 86,521,573 bp
  • T to A, chromosome 9 at 88,583,043 bp
  • C to T, chromosome 10 at 88,791,745 bp
  • T to A, chromosome 10 at 120,054,871 bp
  • A to T, chromosome 10 at 129,527,105 bp
  • T to C, chromosome 10 at 129,912,063 bp
  • A to T, chromosome 11 at 58,625,956 bp
  • T to C, chromosome 11 at 94,242,359 bp
  • A to G, chromosome 11 at 108,855,828 bp
  • A to C, chromosome 11 at 115,160,661 bp
  • T to C, chromosome 11 at 120,377,638 bp
  • T to C, chromosome 12 at 104,130,711 bp
  • C to A, chromosome 13 at 91,859,944 bp
  • C to A, chromosome 14 at 49,073,557 bp
  • T to A, chromosome 14 at 56,635,755 bp
  • A to T, chromosome 14 at 63,919,206 bp
  • A to G, chromosome 14 at 121,581,436 bp
  • G to T, chromosome 14 at 122,978,289 bp
  • A to G, chromosome 15 at 8,201,244 bp
  • C to T, chromosome 15 at 10,329,023 bp
  • T to A, chromosome 15 at 37,937,351 bp
  • T to C, chromosome 15 at 74,723,500 bp
  • T to C, chromosome 16 at 20,552,632 bp
  • A to T, chromosome 16 at 23,470,378 bp
  • C to T, chromosome 16 at 32,756,396 bp
  • A to T, chromosome 16 at 45,095,082 bp
  • G to T, chromosome 17 at 24,641,899 bp
  • T to A, chromosome 17 at 55,592,853 bp
  • T to C, chromosome 17 at 74,649,696 bp
  • T to A, chromosome 18 at 64,483,494 bp
  • C to T, chromosome 19 at 13,633,569 bp
  • T to C, chromosome 19 at 46,308,223 bp
  • G to A, chromosome 19 at 55,203,154 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7539 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045611-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.