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Strain Name:
C57BL/6J-MtgxR7592Btlr/Mmmh
Stock Number:
045639-MU
Citation ID:
RRID:MMRRC_045639-MU
Other Names:
R7592 (G1)
Major Collection:

Strain Information

Npr1
Name: natriuretic peptide receptor 1
Synonyms: NPRA, GC-A, NPR-A, guanylyl cyclase-A
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18160
HGNC: HGNC:7943
Homologene: 37367
Sez6
Name: seizure related gene 6
Synonyms: sez-6, D11Bhm177e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20370
Homologene: 10948
Abca12
Name: ATP-binding cassette, sub-family A member 12
Synonyms: 4832428G11Rik, 4833417A11Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74591
Homologene: 45441
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Prex2
Name: phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2
Synonyms: 6230420N16Rik, C030045D06Rik, Depdc2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 109294
Homologene: 23523
Supt20
Name: SPT20 SAGA complex component
Synonyms: p38 interacting protein, p38IP, D3Ertd300e, Fam48a
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 56790
Homologene: 134155
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 11,123,213 bp
  • A to T, chromosome 1 at 17,728,766 bp
  • T to C, chromosome 1 at 71,288,677 bp
  • A to G, chromosome 1 at 82,648,617 bp
  • T to A, chromosome 1 at 88,088,182 bp
  • A to G, chromosome 3 at 54,707,122 bp
  • C to A, chromosome 3 at 89,353,292 bp
  • T to G, chromosome 3 at 90,465,016 bp
  • T to A, chromosome 3 at 125,570,787 bp
  • A to T, chromosome 4 at 91,311,571 bp
  • T to A, chromosome 4 at 115,023,808 bp
  • T to C, chromosome 4 at 123,410,893 bp
  • A to G, chromosome 4 at 128,463,798 bp
  • A to G, chromosome 4 at 139,360,566 bp
  • A to G, chromosome 5 at 30,341,716 bp
  • T to C, chromosome 5 at 44,063,127 bp
  • A to G, chromosome 5 at 123,997,973 bp
  • T to G, chromosome 6 at 43,217,245 bp
  • T to C, chromosome 6 at 52,191,540 bp
  • C to A, chromosome 6 at 66,804,444 bp
  • C to T, chromosome 7 at 7,000,007 bp
  • G to A, chromosome 7 at 65,658,871 bp
  • G to A, chromosome 7 at 66,789,629 bp
  • A to G, chromosome 7 at 82,337,251 bp
  • GAAAA to GAAAAA, chromosome 7 at 85,411,560 bp
  • C to A, chromosome 7 at 103,755,072 bp
  • A to T, chromosome 7 at 114,269,726 bp
  • A to T, chromosome 8 at 107,050,229 bp
  • C to A, chromosome 9 at 19,477,832 bp
  • T to A, chromosome 9 at 20,797,393 bp
  • A to T, chromosome 9 at 106,349,768 bp
  • A to G, chromosome 10 at 22,664,903 bp
  • A to G, chromosome 11 at 67,774,758 bp
  • A to T, chromosome 11 at 69,223,324 bp
  • A to C, chromosome 11 at 77,476,501 bp
  • T to C, chromosome 11 at 77,978,050 bp
  • T to A, chromosome 11 at 117,290,662 bp
  • T to A, chromosome 12 at 76,804,449 bp
  • G to A, chromosome 12 at 86,161,190 bp
  • T to C, chromosome 12 at 108,186,465 bp
  • G to A, chromosome 13 at 96,947,161 bp
  • T to A, chromosome 13 at 112,695,355 bp
  • A to G, chromosome 14 at 30,908,765 bp
  • T to A, chromosome 14 at 50,186,563 bp
  • G to T, chromosome 14 at 53,876,540 bp
  • T to A, chromosome 14 at 54,711,841 bp
  • A to G, chromosome 15 at 80,210,992 bp
  • A to G, chromosome 16 at 4,849,274 bp
  • A to T, chromosome 16 at 36,367,500 bp
  • T to C, chromosome 17 at 3,171,222 bp
  • C to A, chromosome 17 at 6,289,020 bp
  • G to A, chromosome 17 at 19,591,181 bp
  • T to C, chromosome 17 at 59,006,594 bp
  • A to T, chromosome 18 at 50,567,982 bp
  • T to C, chromosome 18 at 84,014,048 bp
  • T to C, chromosome 19 at 6,014,439 bp
  • T to C, chromosome 19 at 36,056,892 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7592 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045639-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.