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Strain Name:
C57BL/6J-MtgxR7658Btlr/Mmmh
Stock Number:
045703-MU
Citation ID:
RRID:MMRRC_045703-MU
Other Names:
R7658 (G1)
Major Collection:

Strain Information

Mafb
Name: MAF bZIP transcription factor B
Synonyms: Kreisler, Krml1, Krml
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16658
HGNC: HGNC:6408
Homologene: 31315
Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61
Vldlr
Name: very low density lipoprotein receptor
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 22359
Homologene: 443
Nsd1
Name: nuclear receptor-binding SET-domain protein 1
Synonyms: KMT3B
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18193
VEGA: 13
Homologene: 32543
Mthfd1
Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase
Synonyms: Mthfd, DCS, E430024A07Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 108156
VEGA: 12
HGNC: HGNC:7432
Homologene: 55940
Rai14
Name: retinoic acid induced 14
Synonyms: 1700020L11Rik, Ankycorbin, Norpeg, 1700008J19Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 75646
VEGA: 15
Homologene: 9199
Arhgap39
Name: Rho GTPase activating protein 39
Synonyms: D15Wsu169e
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223666
Homologene: 27825
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 58,992,726 bp
  • A to T, chromosome 1 at 87,419,138 bp
  • T to C, chromosome 1 at 143,770,873 bp
  • T to A, chromosome 2 at 24,436,511 bp
  • A to C, chromosome 2 at 44,142,268 bp
  • T to G, chromosome 2 at 76,723,769 bp
  • T to A, chromosome 2 at 160,366,435 bp
  • G to T, chromosome 3 at 7,604,082 bp
  • C to A, chromosome 3 at 90,211,993 bp
  • A to G, chromosome 3 at 92,686,047 bp
  • A to T, chromosome 3 at 94,944,102 bp
  • A to T, chromosome 3 at 110,250,385 bp
  • T to C, chromosome 4 at 47,245,591 bp
  • C to A, chromosome 4 at 98,688,179 bp
  • T to A, chromosome 4 at 117,890,281 bp
  • A to G, chromosome 4 at 133,591,376 bp
  • T to C, chromosome 4 at 147,332,294 bp
  • A to G, chromosome 4 at 155,842,963 bp
  • A to T, chromosome 5 at 3,596,244 bp
  • C to G, chromosome 5 at 3,968,745 bp
  • T to G, chromosome 5 at 15,527,925 bp
  • A to G, chromosome 5 at 24,141,286 bp
  • A to T, chromosome 5 at 30,197,206 bp
  • C to G, chromosome 5 at 93,273,343 bp
  • T to A, chromosome 5 at 103,629,190 bp
  • C to T, chromosome 5 at 114,766,489 bp
  • T to A, chromosome 5 at 129,865,923 bp
  • T to C, chromosome 5 at 137,732,974 bp
  • C to T, chromosome 6 at 34,832,508 bp
  • A to T, chromosome 6 at 36,523,249 bp
  • A to T, chromosome 6 at 57,158,898 bp
  • T to C, chromosome 6 at 87,845,276 bp
  • T to A, chromosome 6 at 100,783,143 bp
  • T to A, chromosome 6 at 104,650,483 bp
  • T to C, chromosome 6 at 113,458,982 bp
  • A to G, chromosome 7 at 4,720,759 bp
  • A to T, chromosome 7 at 6,709,610 bp
  • T to C, chromosome 7 at 41,288,710 bp
  • A to C, chromosome 7 at 42,925,269 bp
  • A to T, chromosome 7 at 43,433,630 bp
  • T to G, chromosome 7 at 45,882,977 bp
  • G to T, chromosome 7 at 76,766,369 bp
  • T to C, chromosome 7 at 97,529,253 bp
  • C to T, chromosome 7 at 100,250,328 bp
  • A to G, chromosome 7 at 101,511,581 bp
  • C to T, chromosome 7 at 119,857,668 bp
  • T to C, chromosome 7 at 139,976,618 bp
  • A to T, chromosome 7 at 142,512,096 bp
  • A to G, chromosome 8 at 91,666,322 bp
  • T to C, chromosome 8 at 104,129,401 bp
  • T to A, chromosome 8 at 122,893,664 bp
  • G to A, chromosome 8 at 125,492,290 bp
  • A to T, chromosome 9 at 7,386,675 bp
  • G to A, chromosome 9 at 31,054,079 bp
  • T to C, chromosome 9 at 42,992,536 bp
  • A to T, chromosome 9 at 72,324,072 bp
  • A to G, chromosome 9 at 105,644,511 bp
  • A to C, chromosome 9 at 108,297,231 bp
  • A to G, chromosome 9 at 119,972,403 bp
  • C to A, chromosome 10 at 86,981,135 bp
  • T to C, chromosome 11 at 52,027,182 bp
  • T to C, chromosome 11 at 62,725,263 bp
  • G to A, chromosome 11 at 69,226,229 bp
  • T to A, chromosome 11 at 70,215,317 bp
  • T to A, chromosome 11 at 95,028,965 bp
  • A to T, chromosome 11 at 99,349,486 bp
  • T to A, chromosome 11 at 109,935,717 bp
  • A to T, chromosome 11 at 115,923,276 bp
  • T to C, chromosome 12 at 24,653,671 bp
  • T to A, chromosome 12 at 76,270,435 bp
  • G to A, chromosome 12 at 115,834,111 bp
  • T to G, chromosome 12 at 116,722,119 bp
  • A to T, chromosome 13 at 13,730,476 bp
  • A to T, chromosome 13 at 55,277,639 bp
  • A to T, chromosome 14 at 34,311,157 bp
  • C to A, chromosome 14 at 55,887,139 bp
  • T to A, chromosome 14 at 115,428,208 bp
  • C to T, chromosome 15 at 10,593,103 bp
  • T to C, chromosome 15 at 63,825,054 bp
  • A to T, chromosome 15 at 76,737,417 bp
  • T to A, chromosome 15 at 91,700,358 bp
  • T to A, chromosome 15 at 98,596,067 bp
  • A to G, chromosome 15 at 99,939,844 bp
  • G to A, chromosome 15 at 102,280,877 bp
  • A to G, chromosome 16 at 23,356,228 bp
  • A to T, chromosome 16 at 36,046,400 bp
  • T to C, chromosome 16 at 50,590,481 bp
  • G to T, chromosome 16 at 89,063,730 bp
  • G to C, chromosome 17 at 33,918,497 bp
  • A to T, chromosome 17 at 71,508,663 bp
  • A to G, chromosome 18 at 37,498,981 bp
  • G to T, chromosome 18 at 57,932,524 bp
  • T to A, chromosome 19 at 3,413,309 bp
  • T to G, chromosome 19 at 6,251,263 bp
  • T to G, chromosome 19 at 17,398,682 bp
  • G to A, chromosome 19 at 27,243,136 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7658 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045703-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.