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Strain Name:
C57BL/6J-MtgxR7665Btlr/Mmmh
Stock Number:
045739-MU
Citation ID:
RRID:MMRRC_045739-MU
Other Names:
R7665 (G1)
Major Collection:

Strain Information

Col2a1
Name: collagen, type II, alpha 1
Synonyms: Del1, Col2a-1, Col2a, Col2, M100856, Rgsc856, Lpk, M100413, Rgsc413
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 12824
HGNC: HGNC:2200
Homologene: 55607
Pik3cd
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta
Synonyms: p110delta, 2410099E07Rik, 2610208K16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18707
HGNC: HGNC:8977
Homologene: 3686
Epb41l4a
Name: erythrocyte membrane protein band 4.1 like 4a
Synonyms: NBL4, Epb4.1l4a
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 13824
VEGA: 18
Homologene: 8398
Arid5b
Name: AT-rich interaction domain 5B
Synonyms: 5430435G07Rik, Desrt, Mrf2beta, Mrf2alpha, Mrf2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 71371
VEGA: 10
Homologene: 45872
Itsn1
Name: intersectin 1 (SH3 domain protein 1A)
Synonyms: Ese1, EHSH1, Sh3p17, Eh domain, SH3 domain regulator of endocytosis 1, Intersectin-L
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 16443
HGNC: HGNC:6183
Homologene: 2277
Enpp2
Name: ectonucleotide pyrophosphatase/phosphodiesterase 2
Synonyms: Autotaxin, Npps2, PD-Ialpha, Pdnp2, ATX
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 18606
HGNC: HGNC:3357
Homologene: 4526
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 11,972,099 bp
  • A to T, chromosome 1 at 181,134,944 bp
  • T to A, chromosome 2 at 82,981,805 bp
  • A to T, chromosome 2 at 122,066,267 bp
  • A to G, chromosome 2 at 164,754,141 bp
  • C to T, chromosome 3 at 38,889,178 bp
  • C to T, chromosome 3 at 122,044,490 bp
  • A to G, chromosome 4 at 6,385,144 bp
  • C to A, chromosome 4 at 117,213,741 bp
  • A to C, chromosome 4 at 118,411,656 bp
  • C to T, chromosome 4 at 149,654,050 bp
  • G to A, chromosome 5 at 8,397,867 bp
  • T to A, chromosome 5 at 27,765,738 bp
  • T to C, chromosome 5 at 31,297,555 bp
  • T to A, chromosome 5 at 76,543,573 bp
  • A to G, chromosome 5 at 114,874,323 bp
  • T to C, chromosome 6 at 35,177,620 bp
  • A to G, chromosome 6 at 42,356,162 bp
  • A to T, chromosome 6 at 89,324,538 bp
  • A to C, chromosome 6 at 100,573,756 bp
  • G to A, chromosome 7 at 6,269,892 bp
  • A to G, chromosome 7 at 28,916,207 bp
  • C to A, chromosome 7 at 56,153,155 bp
  • T to A, chromosome 7 at 85,151,988 bp
  • A to T, chromosome 7 at 103,561,316 bp
  • A to T, chromosome 7 at 106,886,673 bp
  • T to A, chromosome 7 at 122,990,032 bp
  • T to C, chromosome 7 at 122,994,686 bp
  • G to T, chromosome 7 at 140,945,469 bp
  • A to G, chromosome 7 at 143,460,634 bp
  • T to G, chromosome 8 at 13,177,103 bp
  • A to G, chromosome 8 at 85,764,350 bp
  • A to G, chromosome 9 at 58,925,795 bp
  • T to A, chromosome 9 at 65,078,718 bp
  • A to G, chromosome 9 at 73,680,474 bp
  • T to C, chromosome 9 at 76,490,875 bp
  • T to A, chromosome 9 at 104,005,393 bp
  • A to G, chromosome 9 at 121,909,308 bp
  • T to C, chromosome 10 at 21,996,662 bp
  • T to A, chromosome 10 at 29,196,397 bp
  • C to T, chromosome 10 at 68,098,587 bp
  • T to A, chromosome 10 at 130,211,261 bp
  • A to T, chromosome 11 at 73,798,961 bp
  • A to T, chromosome 11 at 94,013,654 bp
  • T to A, chromosome 11 at 117,720,951 bp
  • T to A, chromosome 12 at 8,317,935 bp
  • T to A, chromosome 12 at 40,069,415 bp
  • T to A, chromosome 12 at 51,961,530 bp
  • T to C, chromosome 12 at 85,296,562 bp
  • G to A, chromosome 13 at 17,772,553 bp
  • A to G, chromosome 13 at 41,316,309 bp
  • A to T, chromosome 13 at 76,055,974 bp
  • C to T, chromosome 13 at 81,499,142 bp
  • G to T, chromosome 14 at 69,249,579 bp
  • T to A, chromosome 15 at 54,839,394 bp
  • T to C, chromosome 15 at 81,407,419 bp
  • T to C, chromosome 15 at 84,337,801 bp
  • T to C, chromosome 15 at 97,976,700 bp
  • C to T, chromosome 16 at 32,680,526 bp
  • T to C, chromosome 16 at 91,841,603 bp
  • G to A, chromosome 16 at 96,041,343 bp
  • A to G, chromosome 17 at 21,463,581 bp
  • G to T, chromosome 17 at 33,199,629 bp
  • A to G, chromosome 17 at 37,111,391 bp
  • A to C, chromosome 17 at 50,607,157 bp
  • A to G, chromosome 17 at 55,997,909 bp
  • G to A, chromosome 17 at 57,297,086 bp
  • A to G, chromosome 18 at 34,006,016 bp
  • A to T, chromosome 19 at 5,651,671 bp
  • T to C, chromosome 19 at 8,968,289 bp
  • C to A, chromosome 19 at 12,463,094 bp
  • T to A, chromosome 19 at 34,594,883 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7665 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045739-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.