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Strain Name:
C57BL/6J-MtgxR7694Btlr/Mmmh
Stock Number:
045757-MU
Citation ID:
RRID:MMRRC_045757-MU
Other Names:
R7694 (G1)
Major Collection:

Strain Information

Chrna4
Name: cholinergic receptor, nicotinic, alpha polypeptide 4
Synonyms: alpha4 nAChR, Acra-4, Acra4, a4 nicotinic receptor, alpha4-nAChR
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11438
HGNC: HGNC:1958
Homologene: 592
Ptprk
Name: protein tyrosine phosphatase receptor type K
Synonyms: PTPk, RPTPkappa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19272
VEGA: 10
HGNC: HGNC:9674
Homologene: 55693
Gtf2i
Name: general transcription factor II I
Synonyms: TFII-I, BAP-135, 6030441I21Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14886
HGNC: HGNC:4659
Homologene: 7748
Lin7c
Name: lin-7 homolog C, crumbs cell polarity complex component
Synonyms: LIN-7-C, LIN-7C, Lin7c, MALS-3, Veli3, D2Ertd520e, 9130007B12Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22343
Homologene: 22649
Pelp1
Name: proline, glutamic acid and leucine rich protein 1
Synonyms: 4930563C04Rik, MNAR
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 75273
Homologene: 8664
Ggnbp2
Name: gametogenetin binding protein 2
Synonyms: DIF-3, Zfp403, D330017P12Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217039
Homologene: 11750
Afdn
Name: afadin, adherens junction formation factor
Synonyms: AF6, Afadin, S-afadin, I-afadin, 5033403D15Rik, Mllt4
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 17356
HGNC: HGNC:7137
Homologene: 21202
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 56,871,524 bp
  • T to A, chromosome 1 at 58,752,807 bp
  • G to T, chromosome 1 at 75,201,792 bp
  • A to G, chromosome 2 at 32,388,030 bp
  • T to A, chromosome 2 at 76,747,695 bp
  • T to C, chromosome 2 at 109,896,272 bp
  • C to A, chromosome 2 at 121,377,096 bp
  • C to T, chromosome 2 at 150,239,348 bp
  • C to T, chromosome 2 at 163,469,972 bp
  • T to G, chromosome 2 at 174,300,212 bp
  • A to T, chromosome 2 at 178,460,103 bp
  • T to A, chromosome 2 at 181,018,593 bp
  • A to T, chromosome 3 at 82,358,983 bp
  • T to C, chromosome 3 at 83,129,482 bp
  • C to T, chromosome 4 at 137,743,809 bp
  • T to C, chromosome 4 at 141,905,064 bp
  • A to T, chromosome 5 at 72,580,850 bp
  • C to T, chromosome 5 at 134,282,805 bp
  • T to A, chromosome 6 at 13,078,106 bp
  • A to T, chromosome 6 at 53,932,517 bp
  • A to C, chromosome 6 at 113,690,898 bp
  • G to A, chromosome 6 at 116,133,844 bp
  • G to A, chromosome 6 at 129,270,045 bp
  • G to A, chromosome 7 at 20,872,412 bp
  • C to T, chromosome 7 at 24,142,100 bp
  • A to C, chromosome 7 at 28,301,745 bp
  • C to A, chromosome 7 at 76,698,765 bp
  • TCCCAGG to T, chromosome 7 at 80,098,331 bp
  • T to C, chromosome 7 at 86,269,775 bp
  • G to A, chromosome 7 at 89,930,346 bp
  • T to C, chromosome 7 at 97,971,939 bp
  • A to T, chromosome 7 at 126,467,757 bp
  • G to T, chromosome 8 at 44,988,930 bp
  • T to A, chromosome 8 at 92,831,730 bp
  • A to T, chromosome 8 at 105,894,798 bp
  • G to A, chromosome 9 at 37,418,520 bp
  • A to G, chromosome 9 at 102,898,780 bp
  • T to A, chromosome 10 at 28,589,370 bp
  • T to A, chromosome 10 at 64,088,039 bp
  • T to A, chromosome 10 at 116,372,948 bp
  • G to T, chromosome 11 at 34,050,924 bp
  • C to T, chromosome 11 at 45,933,941 bp
  • G to A, chromosome 11 at 70,394,759 bp
  • A to G, chromosome 11 at 84,860,713 bp
  • C to T, chromosome 11 at 103,504,378 bp
  • A to T, chromosome 12 at 44,902,027 bp
  • T to C, chromosome 12 at 98,792,563 bp
  • G to A, chromosome 12 at 102,561,347 bp
  • A to G, chromosome 13 at 54,682,028 bp
  • G to T, chromosome 13 at 55,413,408 bp
  • T to C, chromosome 13 at 112,367,964 bp
  • A to G, chromosome 14 at 26,781,380 bp
  • A to C, chromosome 14 at 46,853,958 bp
  • T to A, chromosome 16 at 8,645,390 bp
  • T to G, chromosome 17 at 13,888,882 bp
  • A to G, chromosome 17 at 34,205,697 bp
  • T to C, chromosome 17 at 36,773,749 bp
  • A to G, chromosome 17 at 53,400,808 bp
  • A to G, chromosome 18 at 36,536,849 bp
  • G to A, chromosome 19 at 34,670,635 bp
  • G to A, chromosome 19 at 37,701,064 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7694 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045757-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.