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Strain Name:
C57BL/6J-MtgxR7700Btlr/Mmmh
Stock Number:
045761-MU
Citation ID:
RRID:MMRRC_045761-MU
Other Names:
R7700 (G1)
Major Collection:

Strain Information

Rpl13a
Name: ribosomal protein L13A
Synonyms: tum-antigen, tum-transplantation antigen P198, Tstap198-7, 1810026N22Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22121
Homologene: 111053
Acadl
Name: acyl-Coenzyme A dehydrogenase, long-chain
Synonyms: LCAD, C79855
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 11363
HGNC: HGNC:88
Homologene: 37498
App
Name: amyloid beta precursor protein
Synonyms: betaAPP, Abeta, protease nexin II, Adap, Cvap, appican, E030013M08Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 11820
VEGA: 16
HGNC: HGNC:620
Homologene: 56379
Ptpn14
Name: protein tyrosine phosphatase, non-receptor type 14
Synonyms: PTP36, C130080N23Rik, OTTMUSG00000022087
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19250
HGNC: HGNC:9647
Homologene: 3941
Ep400
Name: E1A binding protein p400
Synonyms: p400, mDomino, 1700020J09Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 75560
Homologene: 38779
Mtmr7
Name: myotubularin related protein 7
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 54384
HGNC: HGNC:7454
Homologene: 99732
Dop1b
Name: DOP1 leucine zipper like protein B
Synonyms: 0610038M01Rik, 2610510B01Rik, Dopey2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 70028
VEGA: 16
HGNC: HGNC:1291
Homologene: 21068
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 10,194,411 bp
  • A to G, chromosome 1 at 43,970,466 bp
  • A to G, chromosome 1 at 66,838,363 bp
  • T to C, chromosome 1 at 134,996,032 bp
  • A to G, chromosome 1 at 170,301,148 bp
  • A to T, chromosome 1 at 189,865,411 bp
  • A to T, chromosome 2 at 13,348,178 bp
  • A to G, chromosome 2 at 13,489,917 bp
  • G to A, chromosome 2 at 53,036,508 bp
  • T to C, chromosome 2 at 97,630,679 bp
  • T to A, chromosome 2 at 120,039,716 bp
  • T to C, chromosome 2 at 121,371,748 bp
  • T to A, chromosome 3 at 36,974,172 bp
  • C to T, chromosome 3 at 37,026,154 bp
  • G to A, chromosome 3 at 90,421,804 bp
  • T to G, chromosome 3 at 107,195,540 bp
  • C to T, chromosome 3 at 145,648,692 bp
  • T to A, chromosome 4 at 32,741,344 bp
  • T to A, chromosome 4 at 105,010,454 bp
  • G to T, chromosome 4 at 108,174,077 bp
  • G to T, chromosome 4 at 128,545,756 bp
  • C to A, chromosome 4 at 139,408,867 bp
  • A to T, chromosome 5 at 26,000,180 bp
  • A to T, chromosome 5 at 72,786,024 bp
  • T to A, chromosome 5 at 77,340,421 bp
  • C to T, chromosome 5 at 110,696,032 bp
  • T to C, chromosome 5 at 122,594,560 bp
  • T to A, chromosome 5 at 150,405,327 bp
  • T to C, chromosome 6 at 40,895,388 bp
  • T to C, chromosome 6 at 43,274,757 bp
  • T to A, chromosome 6 at 48,597,822 bp
  • T to A, chromosome 6 at 48,723,857 bp
  • T to A, chromosome 6 at 65,452,956 bp
  • T to C, chromosome 6 at 115,935,239 bp
  • T to C, chromosome 6 at 120,493,427 bp
  • T to C, chromosome 6 at 123,839,923 bp
  • A to G, chromosome 7 at 10,527,632 bp
  • G to T, chromosome 7 at 16,177,556 bp
  • C to T, chromosome 7 at 25,329,928 bp
  • T to C, chromosome 7 at 28,184,312 bp
  • C to A, chromosome 7 at 41,286,407 bp
  • T to G, chromosome 7 at 45,127,236 bp
  • A to C, chromosome 7 at 45,792,574 bp
  • T to A, chromosome 7 at 108,645,672 bp
  • G to A, chromosome 7 at 141,254,929 bp
  • G to A, chromosome 8 at 40,606,884 bp
  • T to A, chromosome 8 at 41,083,969 bp
  • A to T, chromosome 8 at 64,093,954 bp
  • A to T, chromosome 9 at 13,621,089 bp
  • A to T, chromosome 9 at 95,875,690 bp
  • A to G, chromosome 9 at 108,576,361 bp
  • A to G, chromosome 10 at 77,770,635 bp
  • A to T, chromosome 10 at 78,443,930 bp
  • A to T, chromosome 10 at 120,978,090 bp
  • T to A, chromosome 10 at 128,515,854 bp
  • T to C, chromosome 10 at 129,160,055 bp
  • A to G, chromosome 11 at 49,524,554 bp
  • A to T, chromosome 11 at 51,712,257 bp
  • A to G, chromosome 11 at 68,528,563 bp
  • A to T, chromosome 11 at 72,489,617 bp
  • A to T, chromosome 12 at 84,043,237 bp
  • G to A, chromosome 12 at 100,130,835 bp
  • T to G, chromosome 13 at 9,659,311 bp
  • C to T, chromosome 14 at 31,352,444 bp
  • C to T, chromosome 14 at 37,044,595 bp
  • T to A, chromosome 14 at 50,425,335 bp
  • A to G, chromosome 14 at 51,255,791 bp
  • A to C, chromosome 14 at 72,455,902 bp
  • T to A, chromosome 14 at 105,287,759 bp
  • C to T, chromosome 15 at 6,772,154 bp
  • T to A, chromosome 15 at 74,835,842 bp
  • T to G, chromosome 15 at 76,090,897 bp
  • C to A, chromosome 15 at 82,064,105 bp
  • G to A, chromosome 15 at 98,843,719 bp
  • T to A, chromosome 16 at 85,040,309 bp
  • T to G, chromosome 16 at 93,798,761 bp
  • T to C, chromosome 17 at 24,880,574 bp
  • T to C, chromosome 17 at 28,167,049 bp
  • T to A, chromosome 17 at 65,265,218 bp
  • A to G, chromosome 18 at 36,931,062 bp
  • A to G, chromosome 18 at 57,563,316 bp
  • A to G, chromosome 18 at 67,225,704 bp
  • T to C, chromosome 19 at 8,890,053 bp
  • A to G, chromosome 19 at 24,942,681 bp
  • T to C, chromosome 19 at 36,089,158 bp
  • T to G, chromosome 19 at 40,249,658 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7700 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045761-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.