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Strain Name:
C57BL/6J-MtgxR7745Btlr/Mmmh
Stock Number:
045801-MU
Citation ID:
RRID:MMRRC_045801-MU
Other Names:
R7745 (G1)
Major Collection:

Strain Information

Prkar1a
Name: protein kinase, cAMP dependent regulatory, type I, alpha
Synonyms: Tse-1, Tse1, 1300018C22Rik, RIalpha
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 19084
HGNC: HGNC:9388
Homologene: 37664
Jmjd1c
Name: jumonji domain containing 1C
Synonyms: TRIP8, 5430433L24Rik, D630035I23Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 108829
Homologene: 3129
Mgll
Name: monoglyceride lipase
Synonyms: Magl
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 23945
Homologene: 38298
Phf3
Name: PHD finger protein 3
Synonyms: 2310061N19Rik, AU020177
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 213109
HGNC: HGNC:8921
Homologene: 9040
Adgrl2
Name: adhesion G protein-coupled receptor L2
Synonyms: Lphh1, Lec1, Lphn2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99633
Homologene: 22712
Sbno1
Name: strawberry notch 1
Synonyms: sno, 9330180L10Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 243272
Homologene: 10055
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 30,804,224 bp
  • T to C, chromosome 1 at 58,176,517 bp
  • A to G, chromosome 1 at 58,240,707 bp
  • T to C, chromosome 1 at 72,266,998 bp
  • A to G, chromosome 1 at 116,442,283 bp
  • A to T, chromosome 1 at 172,111,942 bp
  • T to A, chromosome 2 at 3,225,446 bp
  • T to A, chromosome 2 at 76,767,800 bp
  • T to G, chromosome 2 at 119,071,556 bp
  • T to C, chromosome 2 at 125,303,195 bp
  • A to T, chromosome 2 at 130,615,068 bp
  • A to C, chromosome 3 at 60,038,472 bp
  • G to T, chromosome 3 at 93,444,777 bp
  • C to A, chromosome 3 at 108,080,116 bp
  • A to G, chromosome 3 at 148,836,458 bp
  • A to G, chromosome 4 at 8,237,939 bp
  • C to T, chromosome 4 at 118,614,931 bp
  • A to G, chromosome 4 at 134,088,845 bp
  • A to G, chromosome 4 at 141,890,939 bp
  • A to T, chromosome 5 at 76,561,512 bp
  • C to G, chromosome 5 at 93,273,343 bp
  • A to T, chromosome 5 at 96,726,895 bp
  • C to G, chromosome 5 at 124,128,705 bp
  • A to T, chromosome 5 at 124,392,899 bp
  • T to A, chromosome 6 at 70,172,015 bp
  • T to C, chromosome 6 at 88,725,788 bp
  • T to C, chromosome 6 at 119,052,626 bp
  • A to T, chromosome 6 at 131,689,438 bp
  • T to A, chromosome 6 at 140,623,858 bp
  • G to T, chromosome 7 at 16,995,689 bp
  • A to G, chromosome 7 at 23,632,610 bp
  • A to G, chromosome 7 at 120,332,217 bp
  • T to C, chromosome 8 at 4,250,622 bp
  • A to G, chromosome 8 at 15,932,461 bp
  • C to A, chromosome 8 at 84,559,394 bp
  • T to G, chromosome 8 at 94,568,684 bp
  • T to A, chromosome 8 at 111,041,657 bp
  • T to C, chromosome 8 at 124,895,819 bp
  • T to C, chromosome 8 at 125,851,107 bp
  • A to T, chromosome 9 at 36,751,239 bp
  • T to C, chromosome 10 at 67,217,045 bp
  • C to T, chromosome 10 at 92,714,172 bp
  • C to T, chromosome 11 at 3,355,896 bp
  • A to T, chromosome 11 at 50,379,497 bp
  • A to C, chromosome 11 at 59,060,855 bp
  • A to T, chromosome 11 at 69,451,318 bp
  • T to A, chromosome 11 at 80,323,579 bp
  • A to T, chromosome 11 at 98,704,967 bp
  • T to A, chromosome 11 at 109,653,847 bp
  • G to T, chromosome 12 at 8,301,854 bp
  • G to A, chromosome 12 at 66,689,350 bp
  • C to A, chromosome 12 at 66,689,351 bp
  • A to T, chromosome 12 at 88,456,800 bp
  • A to T, chromosome 13 at 12,798,297 bp
  • T to A, chromosome 13 at 46,299,601 bp
  • T to A, chromosome 14 at 70,394,189 bp
  • A to T, chromosome 15 at 76,594,548 bp
  • A to T, chromosome 16 at 58,760,419 bp
  • T to G, chromosome 17 at 7,979,243 bp
  • T to C, chromosome 19 at 46,065,342 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7745 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045801-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.