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Strain Name:
C57BL/6J-MtgxR7804Btlr/Mmmh
Stock Number:
045859-MU
Citation ID:
RRID:MMRRC_045859-MU
Other Names:
R7804 (G1)
Major Collection:

Strain Information

Ess2
Name: ess-2 splicing factor
Synonyms: ES2, Dgsi, D16H22S1269E, Es2el, Dgcr14
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 27886
VEGA: 16
Homologene: 11184
Frmd5
Name: FERM domain containing 5
Synonyms: 1500032A09Rik, A930004K21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228564
Homologene: 18206
Neurl4
Name: neuralized E3 ubiquitin protein ligase 4
Synonyms: 0610025P10Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216860
Homologene: 13029
Elovl5
Name: ELOVL fatty acid elongase 5
Synonyms: 1110059L23Rik, ELOVL family member 5, elongation of long chain fatty acids (yeast)
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 68801
Homologene: 41449
Tpr
Name: translocated promoter region, nuclear basket protein
Synonyms: 2610029M07Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 108989
Homologene: 37753
Tpp2
Name: tripeptidyl peptidase II
Synonyms: TppII
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 22019
Homologene: 2471
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 17,624,913 bp
  • T to A, chromosome 1 at 43,983,281 bp
  • A to T, chromosome 1 at 150,432,559 bp
  • A to G, chromosome 1 at 174,195,905 bp
  • A to G, chromosome 1 at 181,182,822 bp
  • C to A, chromosome 2 at 14,968,037 bp
  • G to A, chromosome 2 at 76,707,241 bp
  • C to G, chromosome 2 at 89,001,511 bp
  • A to G, chromosome 2 at 121,591,744 bp
  • G to A, chromosome 2 at 153,002,358 bp
  • A to G, chromosome 4 at 86,991,537 bp
  • A to T, chromosome 4 at 101,782,586 bp
  • A to T, chromosome 4 at 126,512,630 bp
  • C to T, chromosome 5 at 88,328,405 bp
  • A to T, chromosome 5 at 109,220,458 bp
  • T to C, chromosome 5 at 121,424,589 bp
  • C to T, chromosome 6 at 77,641,374 bp
  • T to C, chromosome 6 at 86,424,382 bp
  • A to G, chromosome 6 at 90,203,316 bp
  • G to A, chromosome 6 at 137,399,601 bp
  • C to A, chromosome 7 at 44,312,884 bp
  • A to T, chromosome 7 at 105,204,160 bp
  • A to G, chromosome 7 at 105,566,600 bp
  • A to G, chromosome 7 at 119,952,618 bp
  • A to G, chromosome 7 at 120,011,012 bp
  • T to C, chromosome 8 at 83,236,712 bp
  • C to T, chromosome 9 at 15,990,592 bp
  • T to C, chromosome 9 at 56,619,514 bp
  • A to G, chromosome 9 at 77,979,823 bp
  • T to C, chromosome 10 at 19,849,370 bp
  • T to A, chromosome 10 at 39,823,485 bp
  • A to G, chromosome 10 at 129,776,222 bp
  • GTGTGTATGTGT to GTGTGT, chromosome 11 at 43,587,414 bp
  • C to T, chromosome 11 at 62,205,397 bp
  • T to A, chromosome 11 at 69,905,874 bp
  • C to A, chromosome 11 at 78,154,153 bp
  • T to C, chromosome 11 at 86,212,663 bp
  • T to A, chromosome 11 at 104,584,961 bp
  • T to C, chromosome 11 at 116,003,684 bp
  • C to A, chromosome 11 at 117,812,575 bp
  • C to T, chromosome 12 at 56,612,132 bp
  • A to T, chromosome 13 at 60,725,339 bp
  • T to C, chromosome 14 at 24,165,320 bp
  • A to G, chromosome 14 at 53,538,531 bp
  • A to T, chromosome 15 at 44,597,138 bp
  • T to C, chromosome 16 at 10,542,485 bp
  • T to A, chromosome 16 at 17,911,167 bp
  • T to A, chromosome 17 at 6,019,534 bp
  • A to G, chromosome 17 at 56,258,068 bp
  • G to A, chromosome 17 at 66,555,610 bp
  • A to C, chromosome 17 at 80,615,070 bp
  • A to G, chromosome 18 at 23,595,609 bp
  • A to G, chromosome 18 at 37,738,079 bp
  • G to C, chromosome 18 at 60,829,051 bp
  • T to C, chromosome 19 at 6,986,339 bp
  • C to T, chromosome 19 at 30,578,632 bp
  • G to A, chromosome 19 at 30,624,770 bp
  • A to T, chromosome 19 at 55,228,152 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7804 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045859-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.