Strain Detail Sheet

Strain Name:
B6J;129S4-Kif6tm1c(KOMP)Mbp/RgrayMmnc
Stock Number:
050627-UNC
Citation ID:
RRID:MMRRC_050627-UNC
Other Names:
Kif6tm1c or Kif6tm1c(KOMP)Mbp

Strain Information

Gene Details

Kif6tm1c(KOMP)Wtsi
Name: kinesin family member 6; targeted mutation 1c, Wellcome Trust Sanger Institute
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 17
Kif6
Name: kinesin family member 6
Synonyms: D130004B10Rik, D130084M03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 319991
Homologene: 35308
Genome Browser [Click image to go to Jbrowse]



Additional Resources
IMPC
Google
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PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
KOMP tm1c allele where exon 3 of Kif6 is floxed. Derived from the KOMP EPD0736_3_G01 ES cell clones. Ablates Kif6 function when recombined.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    JM8A3.N1

    Strain Description

    Phenotype
    Targeted knock-in conditional allele of Kif6
    Strain Development
    To create the tm1c allele, the original tm1a, targeted ES cells (EPD0736_3_G01, JM8A3.N1 - C57BL/6N) were injected into B6(Cg)-Tyrc-2J/J (JAX Strain #000058). The chimeras were backcrossed once to B6(Cg)-Tyrc-2J/J screen for integration. One male from this mixed background was backcrossed twice to C57BL/6J (JAX Strain #000664). The offspring were mated with 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J (JAX Strain #003946). Subsequently three backcrosses to C57BL/6J (JAX Strain #000664).
    Suggested Control Mice
    Heterozygous Cre+ or Cre- animals.

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Developmental Biology
    • Models for Human Disease

    Strain Origin

    Donor
    Ryan Gray, Ph.D., University of Texas at Austin Dell Medical School.
    Primary Reference

    Konjikusic MJ, Yeetong P, Boswell CW, Lee C, Roberson EC, Ittiwut R,Suphapeetiporn K, Ciruna B, Gurnett CA, Wallingford JB, Shotelersuk V, Gray RS.Mutations in Kinesin family member 6 reveal specific role in ependymal cellciliogenesis and human neurological development. PLoS Genet. 2018 Nov26;14(11):e1007817. doi: 10.1371/journal.pgen.1007817. [Epub ahead of print](Medline PMID: 30475797)

    Colony and Husbandry Information

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    For more information about this colony's health status contact mmrrc_health@med.unc.edu

    Appearance

    Coat Color
    Agouti
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Backcross
    Generation
    N3 (C57BL/6J)
    Overall Breeding Performance
    Good

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Undetermined Undetermined
    Average litter size
    4-6
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    4-6

    Order Request Information

    Availability Level

    The availability level for this product has not been determined.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Fees

    - Products for this strain are Not Yet Available for Ordering
    - If you register interest in this strain, you will be notified when it becomes available for ordering.
    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.