Strain Name:
B6J;129S4-Kif6tm1c(KOMP)Mbp/RgrayMmnc
Stock Number:
050627-UNC
Citation ID:
RRID:MMRRC_050627-UNC
Other Names:
Kif6tm1c or Kif6tm1c(KOMP)Mbp

Gene Information

Kif6tm1c(KOMP)Wtsi
Name: kinesin family member 6; targeted mutation 1c, Wellcome Trust Sanger Institute
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 17
Kif6
Name: kinesin family member 6
Synonyms: D130004B10Rik, D130084M03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 319991
Homologene: 35308
Genetic Alterations
KOMP tm1c allele where exon 3 of Kif6 is floxed. Derived from the KOMP EPD0736_3_G01 ES cell clones. Ablates Kif6 function when recombined.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    JM8A3.N1
    Phenotype
    Targeted knock-in conditional allele of Kif6
    Strain Development
    To create the tm1c allele, the original tm1a, targeted ES cells (EPD0736_3_G01, JM8A3.N1 - C57BL/6N) were injected into B6(Cg)-Tyrc-2J/J (JAX Strain #000058). The chimeras were backcrossed once to B6(Cg)-Tyrc-2J/J screen for integration. One male from this mixed background was backcrossed twice to C57BL/6J (JAX Strain #000664). The offspring were mated with 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J (JAX Strain #003946). Subsequently three backcrosses to C57BL/6J (JAX Strain #000664).
    Suggested Control Mice
    Heterozygous Cre+ or Cre- animals.
    • Developmental Biology
    • Models for Human Disease
    Donor
    Ryan Gray, Ph.D., University of Texas at Austin Dell Medical School.
    Primary Reference

    Konjikusic MJ, Yeetong P, Boswell CW, Lee C, Roberson EC, Ittiwut R,Suphapeetiporn K, Ciruna B, Gurnett CA, Wallingford JB, Shotelersuk V, Gray RS.Mutations in Kinesin family member 6 reveal specific role in ependymal cellciliogenesis and human neurological development. PLoS Genet. 2018 Nov26;14(11):e1007817. doi: 10.1371/journal.pgen.1007817. [Epub ahead of print](Medline PMID: 30475797)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu .
    Coat Color
    Agouti
    Eye
    Black
    MMRRC Breeding System
    Backcross
    Generation
    N3 (C57BL/6J)
    Overall Breeding Performance
    Good
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Undetermined Undetermined
    Average litter size
    4-6
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    4-6

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    050627-UNC-SPERM Cryo-preserved spermatozoa $548.00 / Non-Profit Aliquot Approximate quantity3
    050627-UNC-RESUS Litter recovered from cryo-archive $2,829.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.