Strain Name:
B6J.Cg-Col1a2oim/McbrLaelMmnc
Stock Number:
066518-UNC
Citation ID:
RRID:MMRRC_066518-UNC
Other Names:
oim (osteogenesis imperfecta murine)

Strain Information

Col1a2oim
Name: collagen, type I, alpha 2; osteogenesis imperfecta
Synonyms: oim
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Spontaneous Mutation
Col1a2
Name: collagen, type I, alpha 2
Synonyms: Col1a-2, Cola2, Cola-2
Type: Gene
Species: Mouse
Chromosome: 6
Alteration at locus: Spontaneous Mutation
NCBI: 12843
HGNC: HGNC:2198
Homologene: 69
Genetic Alterations
Spontaneous deletion of a single base (G) at position 3978 in the Col1a2 gene, predicted to result in a frameshift and subsequent non-sense translation of 48 amino acids.

HGVS nomenclature:

  • Genbank RefSeq - mRNA: NM_007743.3
  • Genbank RefSeq, protein: NP_031769.2
  • Variant, nucleic acid level: c.3978del
  • Variant, amino acid level, predicted: p.Thr1327Glnfs*48
    Check this variant: LUMC Mutalyzer
Note: The positional reference was adjusted from c.3983 (as listed under MGI:1856327) to 3978, based on sequence alignment with PMID:8446583, Fig. 5.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • Phenotype
    Homozygotes have bone fragility, which is used as a model for osteogenesis imperfecta. Other tissues affected are tendon, muscle, and skin.
    MeSH Terms
    • Animals
    • Bone Density/genetics
    • Bone Development/genetics
    • Collagen/deficiency
    • Collagen/genetics
    • Collagen/metabolism
    • Collagen Type I
    • Femur/metabolism
    • Mice
    • Mice, Knockout
    • Phenotype
    • Proline/metabolism
    • Stress, Mechanical
    Strain Development
    Spontaneous
    Suggested Control Mice
    C57BL/6J
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Models for Human Disease
    Donor
    Elizabeth Laird, Ph.D., University of Liverpool.
    Daniel J. McBride, M.D., University of Maryland School of Medicine.
    Charlotte L. Phillips, Ph.D., University of Missouri.
    Primary Reference

    Carleton SM, McBride DJ, Carson WL, Huntington CE, Twenter KL, Rolwes KM,Winkelmann CT, Morris JS, Taylor JF, Phillips CL. Role of genetic background indetermining phenotypic severity throughout postnatal development and at peak bonemass in Col1a2 deficient mice (oim). Bone. 2008 Apr;42(4):681-94. doi:10.1016/j.bone.2007.12.215. Epub 2008 Jan 5. (Medline PMID: 18313376)

    Colony and Husbandry Information

    At 3 weeks of age, suspected homozygous mice are smaller than littermates, may have swollen hind ankles, and the tails may appear thinner with bumps or kinks (suspected fractures). Some heterozygous mice may show signs of swollen hind ankles. Homozygotes with suspected fractures may require analgesia (noted by abnormal gait, limb favoring/lifting and swelling to shoulders and hips). The Donor found that a combination of metacam and buprenorphine worked well in reducing swelling to the joints in the majority of these cases. Scruffing should not be used as a method of restraint for homs/suspected homs, as this can directly cause fractures. Cupping or tunnel handling must be employed with anesthesia used for any procedures. Homozygotes require wet food due to tooth fragility; hard pellets should be removed from the cage at weaning. This can cause the development of overgrown teeth, therefore homozygotes require intermittent monitoring. Elevated cage enrichment is removed to discourage jumping and bone fractures. Ground-based enrichment is however provided.

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Coat Color
    Black
    Eye
    Black
    MMRRC Breeding System
    Backcross and random intra-strain mating
    Generation
    N11+ (C57BL/6)
    Overall Breeding Performance
    Good
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Undetermined Undetermined
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: 4 to 6 months 7 to 9 months
    Average litter size
    7 to 9
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    4 to 6

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    The donor or their institution limits the distribution to non-profit institutions only.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    066518-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.