Strain Detail Sheet

Strain Name:
B6J.B6N(Cg)-Ace2tm1c(KOMP)Wtsi/LazarMmucd
Stock Number:
066973-UCD
Citation ID:
RRID:MMRRC_066973-UCD
Other Names:
mACE2

Gene Information

Gene Details

Ace2tm1c(KOMP)Wtsi
Name: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; targeted mutation 1c, Wellcome Trust Sanger Institute
Synonyms: Ace2flox
Type: Allele
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Conditional
Ace2
Name: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
Synonyms: 2010305L05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Conditional
NCBI: 70008
Homologene: 41448
Genome Browser [Click image to go to Jbrowse]



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Genetic Alterations
Floxed Ace2 allele.
ES Cell Line
JM8A3.N1

Strain Description

Phenotype
The Ace2 gene is floxed. The phenotype depends on the Cre recombinase used. For example, deletion in lung epithelial cells of active ACE2 led to excessive neutrophil accumulation in mouse lungs subjected to bacterial infection, resulting in a hyperinflammatory response and lung damage.

These mice are potentially useful for COVID-19 research.
MeSH Terms
  • ADAM17 Protein/metabolism
  • Angiotensin II/pharmacology
  • Animals
  • Autonomic Nervous System/drug effects
  • Autonomic Nervous System/physiopathology
  • Disease Models, Animal
  • Enzyme Activation/drug effects
  • GABAergic Neurons/metabolism
  • GABAergic Neurons/physiology
  • Hypertension/drug therapy
  • Hypertension/physiopathology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Optogenetics/methods
  • Paraventricular Hypothalamic Nucleus/drug effects
  • Paraventricular Hypothalamic Nucleus/metabolism
  • Paraventricular Hypothalamic Nucleus/pathology
  • Peptidyl-Dipeptidase A/metabolism
  • Random Allocation
  • Renin-Angiotensin System/drug effects
  • Renin-Angiotensin System/genetics
Strain Development

Targeted JM8A3.N1 embryonic stem cells (C57BL/6N-derived) heterozygous for the targeted mutation, clone EPD0613_4_G07 were ordered from the Knockout Mouse Project (KOMP, project ID:79596, UC Davis, CA, USA). Chimeras were bred to albino B6. Subsequent breeding to an Flp deleter strain of unknown genetic background (Bap-flp) removed the frt-pgk-neo-frt sequences. Backcross to C57BL/6J for more than 10 generations.

Suggested Control Mice
C57BL6/J

Research Applications

  • Cardiovascular
  • Cell Biology
  • Diabetes
  • Immunology and Inflammation
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Obesity
  • Research Tools
  • Virology

Strain Origin

Donor
Eric Lazartigues, Ph.D., LSUHSC-NO.
Primary Reference

Mukerjee S, Gao H, Xu J, Sato R, Zsombok A, Lazartigues E. ACE2 and ADAM17Interaction Regulates the Activity of Presympathetic Neurons. Hypertension. 2019 Nov;74(5):1181-1191. doi: 10.1161/HYPERTENSIONAHA.119.13133. Epub 2019 Sep 30.(Medline PMID: 31564162)

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

For more information about this colony's health status contact mmrrc@ucdavis.edu

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Backcross and sib-mating
Generation
N10+ (C57BL/6J), F10+
Overall Breeding Performance
Good

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Undetermined Undetermined
Homozygotes are fertile: Undetermined Undetermined
Hetero/Hemizygotes are fertile: Undetermined Undetermined
Age Reproductive Decline: Undetermined Undetermined
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Availability Level

When this strain becomes available, Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at mmrrc@ucdavis.edu for more details.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.


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