Strain Detail Sheet
- ADAM17 Protein/metabolism
- Angiotensin II/pharmacology
- Animals
- Autonomic Nervous System/drug effects
- Autonomic Nervous System/physiopathology
- Disease Models, Animal
- Enzyme Activation/drug effects
- GABAergic Neurons/metabolism
- GABAergic Neurons/physiology
- Hypertension/drug therapy
- Hypertension/physiopathology
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Optogenetics/methods
- Paraventricular Hypothalamic Nucleus/drug effects
- Paraventricular Hypothalamic Nucleus/metabolism
- Paraventricular Hypothalamic Nucleus/pathology
- Peptidyl-Dipeptidase A/metabolism
- Random Allocation
- Renin-Angiotensin System/drug effects
- Renin-Angiotensin System/genetics
Targeted JM8A3.N1 embryonic stem cells (C57BL/6N-derived) heterozygous for the targeted mutation, clone EPD0613_4_G07 were ordered from the Knockout Mouse Project (KOMP, project ID:79596, UC Davis, CA, USA). Chimeras were bred to albino B6. Subsequent breeding to an Flp deleter strain of unknown genetic background (Bap-flp) removed the frt-pgk-neo-frt sequences. Backcross to C57BL/6J for more than 10 generations.
- Cardiovascular
- Cell Biology
- Diabetes
- Immunology and Inflammation
- Metabolism
- Models for Human Disease
- Neurobiology
- Obesity
- Research Tools
- Virology
Mukerjee S, Gao H, Xu J, Sato R, Zsombok A, Lazartigues E. ACE2 and ADAM17Interaction Regulates the Activity of Presympathetic Neurons. Hypertension. 2019 Nov;74(5):1181-1191. doi: 10.1161/HYPERTENSIONAHA.119.13133. Epub 2019 Sep 30.(Medline PMID: 31564162)
Colony's Current Health Status Report
For more information about this colony's health status contact mmrrc@ucdavis.edu| Viability and Fertility: | Female | Male | Comments |
|---|---|---|---|
| Homozygotes are viable: | Undetermined | Undetermined | |
| Homozygotes are fertile: | Undetermined | Undetermined | |
| Hetero/Hemizygotes are fertile: | Undetermined | Undetermined | |
| Age Reproductive Decline: | Undetermined | Undetermined |
Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at mmrrc@ucdavis.edu for more details.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
| MMRRC Item # | Description | Distribution Fee / Unit (US $) *Shipping & Handling not included* |
Units | Notes |
|---|---|---|---|---|
| 066973-UCD-HET-F 066973-UCD-HET-M |
Heterozygous / Hemizygous Female Heterozygous / Hemizygous Male |
$272.50 / Non-Profit | Per Mouse | The mmrrc@ucdavis.edu may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.). |
| Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information. | ||||
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.