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Strain Name:
C57BL/6J-MtgxR8087Btlr/Mmmh
Stock Number:
067520-MU
Citation ID:
RRID:MMRRC_067520-MU
Other Names:
R8087 (G1)
Major Collection:

Strain Information

Foxp4
Name: forkhead box P4
Synonyms: 1200010K03Rik, 2310007G05Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 74123
Homologene: 12536
Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: E330008K23Rik, HALR, Mll3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231051
Homologene: 46480
Cadps
Name: Ca2+-dependent secretion activator
Synonyms: CAPS1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 27062
VEGA: 14
HGNC: HGNC:1426
Homologene: 2755
Sim1
Name: single-minded family bHLH transcription factor 1
Synonyms: bHLHe14
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 20464
VEGA: 10
Homologene: 3715
Ntsr1
Name: neurotensin receptor 1
Synonyms: NT-1R, Ntsr1, NTR-1, NTR1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18216
HGNC: HGNC:8039
Homologene: 68261
Sstr2
Name: somatostatin receptor 2
Synonyms: SSTR-2, sst2, Smstr2, Smstr-2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20606
Homologene: 37427
Calca
Name: calcitonin/calcitonin-related polypeptide, alpha
Synonyms: Cgrp, CA, alpha CGRP, Ctn, Ct, Calc, CT
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12310
HGNC: HGNC:1437
Homologene: 88337
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 10,104,264 bp
  • G to T, chromosome 1 at 20,523,089 bp
  • A to T, chromosome 1 at 32,414,976 bp
  • T to A, chromosome 1 at 87,916,847 bp
  • G to T, chromosome 2 at 33,045,536 bp
  • T to C, chromosome 2 at 69,448,129 bp
  • A to T, chromosome 2 at 84,610,876 bp
  • A to G, chromosome 2 at 150,642,748 bp
  • T to C, chromosome 2 at 165,353,759 bp
  • A to T, chromosome 2 at 180,500,172 bp
  • G to T, chromosome 3 at 100,964,096 bp
  • G to T, chromosome 4 at 59,592,228 bp
  • A to T, chromosome 4 at 117,165,418 bp
  • GGCAGCAGCAGCAGCAGCA to GGCAGCAGCAGCAGCA, chromosome 5 at 24,438,749 bp
  • T to C, chromosome 5 at 25,329,252 bp
  • T to A, chromosome 5 at 30,917,100 bp
  • C to A, chromosome 5 at 87,846,309 bp
  • T to C, chromosome 5 at 99,221,389 bp
  • T to A, chromosome 5 at 107,485,087 bp
  • T to C, chromosome 5 at 108,388,462 bp
  • A to G, chromosome 5 at 108,486,236 bp
  • T to C, chromosome 5 at 114,412,489 bp
  • T to C, chromosome 5 at 114,719,604 bp
  • T to A, chromosome 5 at 138,190,270 bp
  • T to A, chromosome 6 at 40,418,592 bp
  • T to A, chromosome 6 at 97,185,383 bp
  • G to A, chromosome 6 at 124,513,872 bp
  • G to T, chromosome 6 at 125,233,362 bp
  • A to C, chromosome 7 at 30,234,372 bp
  • A to T, chromosome 7 at 43,047,670 bp
  • A to T, chromosome 7 at 105,753,499 bp
  • T to C, chromosome 7 at 114,632,574 bp
  • A to G, chromosome 7 at 126,830,880 bp
  • A to G, chromosome 7 at 141,087,638 bp
  • G to A, chromosome 8 at 94,654,297 bp
  • A to T, chromosome 8 at 110,719,900 bp
  • A to T, chromosome 8 at 117,108,762 bp
  • T to C, chromosome 9 at 30,464,402 bp
  • A to G, chromosome 9 at 32,257,028 bp
  • C to T, chromosome 9 at 57,248,674 bp
  • A to T, chromosome 9 at 114,430,415 bp
  • A to G, chromosome 9 at 121,266,251 bp
  • A to G, chromosome 9 at 123,964,334 bp
  • C to T, chromosome 10 at 5,333,034 bp
  • T to A, chromosome 10 at 39,529,557 bp
  • T to C, chromosome 10 at 50,909,555 bp
  • T to A, chromosome 10 at 120,182,535 bp
  • T to C, chromosome 10 at 121,601,327 bp
  • A to T, chromosome 11 at 53,996,061 bp
  • A to G, chromosome 11 at 64,979,208 bp
  • A to T, chromosome 11 at 71,172,071 bp
  • A to T, chromosome 11 at 110,490,176 bp
  • G to A, chromosome 11 at 113,624,675 bp
  • T to C, chromosome 11 at 120,897,879 bp
  • A to G, chromosome 13 at 54,996,172 bp
  • C to T, chromosome 13 at 93,703,871 bp
  • A to G, chromosome 13 at 95,513,999 bp
  • A to C, chromosome 14 at 12,536,380 bp
  • G to T, chromosome 14 at 32,785,969 bp
  • A to G, chromosome 15 at 99,941,844 bp
  • T to C, chromosome 16 at 10,420,348 bp
  • G to T, chromosome 16 at 33,799,027 bp
  • C to T, chromosome 16 at 94,442,953 bp
  • A to C, chromosome 17 at 21,434,998 bp
  • A to T, chromosome 17 at 22,958,737 bp
  • T to C, chromosome 17 at 23,837,102 bp
  • G to T, chromosome 17 at 24,512,064 bp
  • T to A, chromosome 17 at 29,294,090 bp
  • T to C, chromosome 17 at 31,064,485 bp
  • G to A, chromosome 17 at 33,381,003 bp
  • T to C, chromosome 17 at 34,035,789 bp
  • T to C, chromosome 17 at 37,111,548 bp
  • C to A, chromosome 17 at 47,904,430 bp
  • T to A, chromosome 17 at 66,149,993 bp
  • A to G, chromosome 17 at 73,316,284 bp
  • C to A, chromosome 18 at 37,308,664 bp
  • A to G, chromosome 19 at 38,736,521 bp
  • A to G, chromosome 19 at 57,182,256 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8087 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067520-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.