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Strain Name:
C57BL/6J-MtgxR8143Btlr/Mmmh
Stock Number:
067571-MU
Citation ID:
RRID:MMRRC_067571-MU
Other Names:
R8143 (G1)
Major Collection:

Strain Information

Pank3
Name: pantothenate kinase 3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 211347
Homologene: 56999
Chrnb2
Name: cholinergic receptor nicotinic beta 2 subunit
Synonyms: [b]2-nAchR, Acrb-2, Acrb2, C030030P04Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11444
HGNC: HGNC:1962
Homologene: 595
Slc1a2
Name: solute carrier family 1 (glial high affinity glutamate transporter), member 2
Synonyms: GLT1, MGLT1, Eaat2, 1700091C19Rik, GLT-1, 2900019G14Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20511
Homologene: 3075
Ebf2
Name: early B cell factor 2
Synonyms: O/E-3, D14Ggc1e, Mmot1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 13592
Homologene: 56471
Fryl
Name: FRY like transcription coactivator
Synonyms: 2310004H21Rik, 2510002A14Rik, 9030227G01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72313
Homologene: 103956
Cyp39a1
Name: cytochrome P450, family 39, subfamily a, polypeptide 1
Synonyms: oxysterol 7-alpha-hydroxylase
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 56050
VEGA: 17
Homologene: 9580
Numa1
Name: nuclear mitotic apparatus protein 1
Synonyms: 6720401E04Rik, NuMA
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101706
HGNC: HGNC:8059
Homologene: 38150
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 58,158,915 bp
  • A to G, chromosome 1 at 150,859,206 bp
  • G to A, chromosome 1 at 172,894,333 bp
  • T to A, chromosome 1 at 173,682,676 bp
  • T to G, chromosome 2 at 22,282,665 bp
  • C to A, chromosome 2 at 35,375,872 bp
  • T to C, chromosome 2 at 76,863,333 bp
  • T to C, chromosome 2 at 80,506,186 bp
  • T to A, chromosome 2 at 102,737,885 bp
  • T to A, chromosome 2 at 130,141,843 bp
  • T to C, chromosome 2 at 150,667,881 bp
  • T to C, chromosome 3 at 33,082,509 bp
  • T to G, chromosome 3 at 36,946,508 bp
  • T to C, chromosome 3 at 89,747,323 bp
  • T to A, chromosome 3 at 95,469,983 bp
  • T to C, chromosome 4 at 86,342,255 bp
  • G to A, chromosome 4 at 99,967,218 bp
  • A to G, chromosome 4 at 109,065,709 bp
  • T to A, chromosome 4 at 134,927,008 bp
  • T to C, chromosome 4 at 152,041,102 bp
  • A to T, chromosome 5 at 30,863,375 bp
  • G to A, chromosome 5 at 73,050,339 bp
  • T to G, chromosome 5 at 123,228,416 bp
  • T to A, chromosome 5 at 144,835,897 bp
  • A to T, chromosome 5 at 150,662,205 bp
  • G to C, chromosome 6 at 29,441,485 bp
  • G to A, chromosome 6 at 66,716,052 bp
  • A to T, chromosome 6 at 123,741,353 bp
  • G to T, chromosome 7 at 4,628,485 bp
  • A to C, chromosome 7 at 5,035,911 bp
  • G to A, chromosome 7 at 27,129,253 bp
  • A to G, chromosome 7 at 101,999,684 bp
  • T to A, chromosome 7 at 119,834,261 bp
  • A to T, chromosome 7 at 127,033,225 bp
  • C to T, chromosome 7 at 142,212,426 bp
  • T to A, chromosome 7 at 144,461,262 bp
  • A to G, chromosome 9 at 19,567,291 bp
  • T to G, chromosome 10 at 19,356,984 bp
  • T to A, chromosome 10 at 41,337,592 bp
  • T to C, chromosome 10 at 81,181,348 bp
  • A to G, chromosome 10 at 107,806,666 bp
  • T to C, chromosome 11 at 3,678,537 bp
  • A to G, chromosome 11 at 35,776,209 bp
  • T to G, chromosome 11 at 98,003,320 bp
  • A to G, chromosome 11 at 101,262,799 bp
  • G to A, chromosome 11 at 115,993,429 bp
  • G to A, chromosome 11 at 116,126,461 bp
  • A to G, chromosome 12 at 4,633,003 bp
  • A to T, chromosome 12 at 104,130,534 bp
  • G to A, chromosome 13 at 3,642,065 bp
  • T to C, chromosome 13 at 56,782,549 bp
  • A to T, chromosome 14 at 20,987,044 bp
  • C to T, chromosome 14 at 65,931,820 bp
  • C to A, chromosome 14 at 67,411,937 bp
  • A to G, chromosome 15 at 100,716,207 bp
  • G to A, chromosome 16 at 34,914,155 bp
  • G to A, chromosome 16 at 38,450,332 bp
  • A to G, chromosome 16 at 43,871,754 bp
  • T to A, chromosome 17 at 36,187,492 bp
  • T to C, chromosome 17 at 43,725,626 bp
  • G to A, chromosome 17 at 78,858,532 bp
  • A to G, chromosome 19 at 5,607,549 bp
  • G to A, chromosome 19 at 47,268,699 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8143 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067571-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.