Strain Name:
C57BL/6J-MtgxR8163Btlr/Mmmh
Stock Number:
067589-MU
Citation ID:
RRID:MMRRC_067589-MU
Other Names:
R8163 (G1)
Major Collection:

Strain Information

Prnp
Name: prion protein
Synonyms: PrP, PrPC, Prn-i, Prn-p, Sinc, PrPSc, CD230
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 19122
HGNC: HGNC:9449
Homologene: 7904
Rc3h1
Name: RING CCCH (C3H) domains 1
Synonyms: roquin, 5730557L09Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 381305
Homologene: 19036
Pzp
Name: PZP, alpha-2-macroglobulin like
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 11287
Homologene: 104112
Cfl1
Name: cofilin 1, non-muscle
Synonyms: cofilin, Cof, n-cofilin
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 12631
HGNC: HGNC:1874
Homologene: 99735
Eif2s2
Name: eukaryotic translation initiation factor 2 subunit 2 beta
Synonyms: EIF2B, EIF2, 2810026E11Rik, 38kDa, D2Ertd303e
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 67204
HGNC: HGNC:3266
Homologene: 2904
Fastk
Name: Fas-activated serine/threonine kinase
Synonyms: 0610011K02Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 66587
Homologene: 4884
Rngtt
Name: RNA guanylyltransferase and 5'-phosphatase
Synonyms: mouse capping enzyme
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 24018
Homologene: 37851
Ddx50
Name: DExD box helicase 50
Synonyms: GU2, RH-II/Gubeta, 4933429B04Rik, 8430408E17Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 50
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 94213
VEGA: 10
Homologene: 56986
Vps13c
Name: vacuolar protein sorting 13C
Synonyms: C230055H22Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 320528
VEGA: 9
Homologene: 41188
Zfhx3
Name: zinc finger homeobox 3
Synonyms: WBP9, A230102L03Rik, Atbf1, Sci
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11906
HGNC: HGNC:777
Homologene: 21366
Lin9
Name: lin-9 DREAM MuvB core complex component
Synonyms: 2700022J23Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 72568
Homologene: 35252
Asap1
Name: ArfGAP with SH3 domain, ankyrin repeat and PH domain1
Synonyms: Ddef1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 13196
HGNC: HGNC:2720
Homologene: 7684
Katnb1
Name: katanin p80 (WD40-containing) subunit B 1
Synonyms: KAT, 2410003J24Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 74187
HGNC: HGNC:6217
Homologene: 4302
Sec16a
Name: SEC16 homolog A, endoplasmic reticulum export factor
Synonyms: C230052J16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227648
Homologene: 10533
Tbcd
Name: tubulin-specific chaperone d
Synonyms: A030005L14Rik, 2310057L06Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 108903
Homologene: 4368
Ago3
Name: argonaute RISC catalytic subunit 3
Synonyms: argonaute 3, eIF2C3, C130014L07Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 214150
Homologene: 49799
Taf6
Name: TATA-box binding protein associated factor 6
Synonyms: p80, Taf2e, 80kDa
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 21343
Homologene: 7561
Ncr1
Name: natural cytotoxicity triggering receptor 1
Synonyms: NKp46, MAR1 (mouse activating receptor 1), Ly94, Cd335
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 17086
HGNC: HGNC:6731
Homologene: 3551
Nktr
Name: natural killer tumor recognition sequence
Synonyms: D9Wsu172e, 5330401F18Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 18087
HGNC: HGNC:7833
Homologene: 122148
Plaa
Name: phospholipase A2, activating protein
Synonyms: D4Ertd618e, Ufd3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18786
HGNC: HGNC:9043
Homologene: 3138
Lap3
Name: leucine aminopeptidase 3
Synonyms: Pep-S, Pep-7, LAP, peptidase S, Pep7
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 66988
Homologene: 41072
Rps10
Name: ribosomal protein S10
Synonyms: 2210402A09Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 67097
Homologene: 788
Fzd5
Name: frizzled class receptor 5
Synonyms: Fz5, 5330434N09Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14367
HGNC: HGNC:4043
Homologene: 2590
Fat3
Name: FAT atypical cadherin 3
Synonyms: LOC234973, LOC382129, 9430076A06Rik, D430038H04Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 270120
VEGA: 9
Homologene: 82252
Wdfy4
Name: WD repeat and FYVE domain containing 4
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 545030
Homologene: 83473
Fat4
Name: FAT atypical cadherin 4
Synonyms: 6030410K14Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 329628
Homologene: 14377
Vmn2r78
Name: vomeronasal 2, receptor 78
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 637896
Homologene: 115466
Scn9a
Name: sodium channel, voltage-gated, type IX, alpha
Synonyms: PN1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20274
Homologene: 2237
Lrrc37
Name: leucine rich repeat containing 37
Synonyms: LOC380730, Gm884
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 380730
Homologene: 134511
Kynu
Name: kynureninase
Synonyms: 4432411A05Rik, L-kynurenine hydrolase
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 70789
HGNC: HGNC:6469
Homologene: 2925
Bbof1
Name: basal body orientation factor 1
Synonyms: 2900006K08Rik, Ccdc176
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 72873
Homologene: 75215
Krt26
Name: keratin 26
Synonyms: 4732407F15Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 320864
Homologene: 37118
Igsf9b
Name: immunoglobulin superfamily, member 9B
Synonyms: LOC235086, AI414108
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235086
Homologene: 19472
Lrrtm2
Name: leucine rich repeat transmembrane neuronal 2
Synonyms: C630011A14Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 107065
VEGA: 18
Homologene: 65342
Zfp692
Name: zinc finger protein 692
Synonyms: Zfp692-ps
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 103836
Homologene: 9889
Iqub
Name: IQ motif and ubiquitin domain containing
Synonyms: 4932408B21Rik, Trs4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 214704
Homologene: 44790
Fbxw13
Name: F-box and WD-40 domain protein 13
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 211305
Homologene: 110776
Il5
Name: interleukin 5
Synonyms: Il-5
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16191
HGNC: HGNC:6016
Homologene: 679
Parp16
Name: poly (ADP-ribose) polymerase family, member 16
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 214424
VEGA: 9
Homologene: 9878
N4bp2l2
Name: NEDD4 binding protein 2-like 2
Synonyms: 2700092H06Rik, zag1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 381695
Homologene: 115380
Cpa2
Name: carboxypeptidase A2, pancreatic
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232680
HGNC: HGNC:2297
Homologene: 37541
Tmem198
Name: transmembrane protein 198
Synonyms: Tmem198-1, Tmem198a, A230078I05Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 319998
Homologene: 44245
9930022D16Rik
Name: RIKEN cDNA 9930022D16 gene
Type: Gene
Species: Mouse
Chromosome: 11
Dnajc28
Name: DnaJ heat shock protein family (Hsp40) member C28
Synonyms: ORF28
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 246738
HGNC: HGNC:1297
Homologene: 9869
Zfp36l2
Name: zinc finger protein 36, C3H type-like 2
Synonyms: Tis11d, ERF2, Brf2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 12193
HGNC: HGNC:1108
Homologene: 5027
Gpr25
Name: G protein-coupled receptor 25
Synonyms: LOC383563
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 383563
HGNC: HGNC:4480
Homologene: 3872
Cd3d
Name: CD3 antigen, delta polypeptide
Synonyms: T3d
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12500
VEGA: 9
HGNC: HGNC:1673
Homologene: 585
Echs1
Name: enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 93747
HGNC: HGNC:3151
Homologene: 3018
Or5b95
Name: olfactory receptor family 5 subfamily B member 95
Synonyms: GA_x6K02T2RE5P-3006492-3007430, MOR202-8, Olfr1443
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 258693
HGNC: HGNC:8324
Homologene: 115496
Krt88
Name: keratin 88
Synonyms: 1700011A15Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 66322
VEGA: 15
Homologene: 11945
Cebpd
Name: CCAAT/enhancer binding protein delta
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12609
HGNC: HGNC:1835
Homologene: 3808
G530012D18Rik
Name: RIKEN cDNA G530012D1 gene
Type: Gene
Species: Mouse
Chromosome: 1
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 64,735,193 bp
  • C to T, chromosome 1 at 75,483,027 bp
  • C to G, chromosome 1 at 85,577,214 bp
  • T to C, chromosome 1 at 136,259,858 bp
  • T to A, chromosome 1 at 160,955,059 bp
  • C to T, chromosome 1 at 180,659,126 bp
  • A to T, chromosome 2 at 26,416,421 bp
  • G to T, chromosome 2 at 43,628,954 bp
  • T to A, chromosome 2 at 66,484,401 bp
  • A to G, chromosome 2 at 131,936,988 bp
  • A to G, chromosome 2 at 154,892,701 bp
  • A to T, chromosome 3 at 38,979,732 bp
  • G to A, chromosome 4 at 33,325,109 bp
  • C to G, chromosome 4 at 94,569,403 bp
  • G to T, chromosome 4 at 126,368,584 bp
  • A to G, chromosome 5 at 24,444,275 bp
  • C to T, chromosome 5 at 45,512,047 bp
  • G to T, chromosome 5 at 138,181,976 bp
  • A to G, chromosome 5 at 150,661,309 bp
  • G to A, chromosome 6 at 24,449,715 bp
  • A to C, chromosome 6 at 30,564,351 bp
  • T to G, chromosome 6 at 128,512,194 bp
  • T to C, chromosome 7 at 4,340,829 bp
  • G to A, chromosome 7 at 86,954,452 bp
  • A to G, chromosome 7 at 140,112,444 bp
  • T to C, chromosome 8 at 95,096,386 bp
  • A to G, chromosome 8 at 108,949,293 bp
  • G to A, chromosome 9 at 15,959,759 bp
  • T to A, chromosome 9 at 27,322,611 bp
  • T to C, chromosome 9 at 44,985,654 bp
  • T to A, chromosome 9 at 65,229,949 bp
  • G to A, chromosome 9 at 67,950,438 bp
  • G to A, chromosome 9 at 109,183,054 bp
  • TAGAAG to TAG, chromosome 9 at 121,750,863 bp
  • C to A, chromosome 10 at 62,639,899 bp
  • T to A, chromosome 11 at 53,723,986 bp
  • T to A, chromosome 11 at 58,310,373 bp
  • T to C, chromosome 11 at 99,329,672 bp
  • A to T, chromosome 11 at 103,615,862 bp
  • A to G, chromosome 11 at 109,414,057 bp
  • C to T, chromosome 11 at 121,493,885 bp
  • T to C, chromosome 12 at 84,426,762 bp
  • C to T, chromosome 14 at 33,151,588 bp
  • A to G, chromosome 15 at 64,092,050 bp
  • T to C, chromosome 15 at 101,453,508 bp
  • A to T, chromosome 16 at 15,887,977 bp
  • G to A, chromosome 16 at 91,616,907 bp
  • G to A, chromosome 17 at 27,634,111 bp
  • T to C, chromosome 17 at 84,187,123 bp
  • C to T, chromosome 18 at 35,213,724 bp
  • T to C, chromosome 19 at 5,493,500 bp
  • A to G, chromosome 19 at 12,680,188 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8163 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067589-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.