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Strain Name:
C57BL/6J-MtgxR8177Btlr/Mmmh
Stock Number:
067602-MU
Citation ID:
RRID:MMRRC_067602-MU
Other Names:
R8177 (G1)
Major Collection:

Strain Information

Col2a1
Name: collagen, type II, alpha 1
Synonyms: Del1, Col2a-1, Col2a, Col2, M100856, Rgsc856, Lpk, M100413, Rgsc413
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 12824
HGNC: HGNC:2200
Homologene: 55607
Col3a1
Name: collagen, type III, alpha 1
Synonyms: Col3a-1, Tsk-2, Tsk2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12825
HGNC: HGNC:2201
Homologene: 55433
Slc17a7
Name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7
Synonyms: Vglut1, 2900052E22Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 72961
Homologene: 113454
Slit3
Name: slit guidance ligand 3
Synonyms: Slit1, b2b2362.1Clo
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20564
Homologene: 2303
Col6a1
Name: collagen, type VI, alpha 1
Synonyms: Col6a-1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12833
HGNC: HGNC:2211
Homologene: 1391
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 32,546,376 bp
  • G to C, chromosome 1 at 45,335,764 bp
  • T to C, chromosome 1 at 71,609,587 bp
  • T to C, chromosome 2 at 38,729,498 bp
  • T to A, chromosome 2 at 86,553,279 bp
  • C to A, chromosome 2 at 87,135,950 bp
  • T to A, chromosome 2 at 87,833,168 bp
  • T to A, chromosome 2 at 110,666,456 bp
  • A to T, chromosome 2 at 134,643,902 bp
  • C to T, chromosome 2 at 144,585,623 bp
  • A to T, chromosome 2 at 149,899,868 bp
  • T to C, chromosome 2 at 164,088,857 bp
  • T to A, chromosome 2 at 180,607,657 bp
  • C to A, chromosome 3 at 57,690,416 bp
  • T to C, chromosome 3 at 97,767,532 bp
  • G to T, chromosome 4 at 41,497,568 bp
  • T to C, chromosome 4 at 136,945,663 bp
  • A to G, chromosome 4 at 152,327,511 bp
  • G to A, chromosome 5 at 21,373,309 bp
  • A to G, chromosome 5 at 34,616,705 bp
  • A to G, chromosome 5 at 45,693,753 bp
  • G to A, chromosome 5 at 108,197,795 bp
  • T to G, chromosome 5 at 115,988,159 bp
  • T to A, chromosome 6 at 58,215,774 bp
  • T to A, chromosome 6 at 69,823,461 bp
  • A to G, chromosome 6 at 91,014,488 bp
  • C to T, chromosome 6 at 121,325,028 bp
  • T to A, chromosome 6 at 142,101,734 bp
  • T to A, chromosome 7 at 19,074,239 bp
  • C to A, chromosome 7 at 26,819,153 bp
  • T to A, chromosome 7 at 28,183,176 bp
  • G to A, chromosome 7 at 35,456,133 bp
  • T to A, chromosome 7 at 45,174,932 bp
  • T to C, chromosome 7 at 67,734,065 bp
  • G to A, chromosome 7 at 81,164,416 bp
  • A to T, chromosome 7 at 85,222,214 bp
  • G to C, chromosome 7 at 107,001,456 bp
  • T to A, chromosome 7 at 126,763,765 bp
  • T to G, chromosome 7 at 127,596,150 bp
  • T to A, chromosome 7 at 131,106,432 bp
  • G to C, chromosome 7 at 141,807,331 bp
  • A to G, chromosome 9 at 22,514,063 bp
  • T to A, chromosome 9 at 30,922,026 bp
  • T to G, chromosome 9 at 103,320,541 bp
  • T to A, chromosome 9 at 107,907,585 bp
  • T to C, chromosome 10 at 76,725,029 bp
  • A to G, chromosome 10 at 80,895,048 bp
  • A to G, chromosome 10 at 129,250,921 bp
  • T to C, chromosome 11 at 6,193,460 bp
  • A to G, chromosome 11 at 35,579,092 bp
  • A to T, chromosome 11 at 99,559,298 bp
  • A to T, chromosome 11 at 103,189,959 bp
  • A to T, chromosome 11 at 118,128,927 bp
  • C to T, chromosome 12 at 118,872,790 bp
  • A to G, chromosome 13 at 4,242,592 bp
  • A to G, chromosome 13 at 17,695,620 bp
  • T to C, chromosome 13 at 22,592,245 bp
  • T to C, chromosome 13 at 25,004,978 bp
  • T to C, chromosome 13 at 50,425,624 bp
  • T to C, chromosome 13 at 100,427,403 bp
  • C to T, chromosome 14 at 72,460,537 bp
  • T to C, chromosome 15 at 78,465,127 bp
  • T to C, chromosome 15 at 94,584,395 bp
  • T to A, chromosome 15 at 97,976,773 bp
  • A to G, chromosome 17 at 21,463,867 bp
  • A to G, chromosome 17 at 22,603,613 bp
  • A to G, chromosome 17 at 46,714,062 bp
  • A to T, chromosome 17 at 48,128,564 bp
  • A to G, chromosome 17 at 71,390,453 bp
  • A to G, chromosome 18 at 38,333,295 bp
  • A to G, chromosome 18 at 42,324,110 bp
  • A to G, chromosome 19 at 43,807,080 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8177 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067602-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.