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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR8348Btlr/Mmmh
Stock Number:
067732-MU
Citation ID:
RRID:MMRRC_067732-MU
Other Names:
R8348 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Hdgfl2
Name: HDGF like 2
Synonyms: HRP-2, Hdgfrp2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 15193
VEGA: 17
Homologene: 7355

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Met
Name: met proto-oncogene
Synonyms: HGF receptor, c-Met, Par4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17295
HGNC: HGNC:7029
Homologene: 206

Additional Resources
IMPC
Google
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Gene Ontology
BioGPS
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Limch1
Name: LIM and calponin homology domains 1
Synonyms: 3732412D22Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 77569
Homologene: 18953

Additional Resources
IMPC
Google
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Gene Ontology
BioGPS
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Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Cdkn2a
Name: cyclin dependent kinase inhibitor 2A
Synonyms: MTS1, p19ARF, p16INK4a, INK4a-ARF, p16, ARF-INK4a, Arf, Pctr1, p19ARF, Ink4a/Arf
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12578
HGNC: HGNC:1787
Homologene: 55430

Additional Resources
IMPC
Google
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IMSR
Gene Ontology
BioGPS
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Angpt2
Name: angiopoietin 2
Synonyms: Ang2, Ang-2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11601
HGNC: HGNC:485
Homologene: 22401

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
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IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Mcoln3
Name: mucolipin 3
Synonyms: varitint-waddler, Va, 6720490O21Rik, TRPML3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 171166
Homologene: 10118

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Ubc
Name: ubiquitin C
Synonyms: 2700054O04Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22190
Homologene: 128418

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 93,576,892 bp
  • A to T, chromosome 1 at 156,418,284 bp
  • A to C, chromosome 1 at 170,301,640 bp
  • T to A, chromosome 1 at 178,256,340 bp
  • T to C, chromosome 2 at 29,942,544 bp
  • A to T, chromosome 2 at 59,911,793 bp
  • A to T, chromosome 2 at 161,558,886 bp
  • T to C, chromosome 2 at 178,256,142 bp
  • T to A, chromosome 3 at 27,439,995 bp
  • T to A, chromosome 3 at 104,051,215 bp
  • T to A, chromosome 3 at 130,629,492 bp
  • A to T, chromosome 3 at 131,112,812 bp
  • T to C, chromosome 3 at 146,131,219 bp
  • T to C, chromosome 3 at 157,925,020 bp
  • A to G, chromosome 4 at 57,240,784 bp
  • T to C, chromosome 4 at 62,542,785 bp
  • C to A, chromosome 4 at 89,282,054 bp
  • A to G, chromosome 4 at 95,844,190 bp
  • A to G, chromosome 4 at 106,368,736 bp
  • A to T, chromosome 4 at 120,930,637 bp
  • G to A, chromosome 4 at 124,785,174 bp
  • G to A, chromosome 4 at 129,223,906 bp
  • A to G, chromosome 4 at 150,619,196 bp
  • A to G, chromosome 4 at 152,360,716 bp
  • G to A, chromosome 5 at 3,948,897 bp
  • T to C, chromosome 5 at 64,953,842 bp
  • A to T, chromosome 5 at 66,027,230 bp
  • A to G, chromosome 5 at 67,002,482 bp
  • G to A, chromosome 5 at 110,104,026 bp
  • C to A, chromosome 5 at 110,242,043 bp
  • T to A, chromosome 5 at 113,572,310 bp
  • A to T, chromosome 5 at 121,220,256 bp
  • C to T, chromosome 5 at 125,388,031 bp
  • T to C, chromosome 5 at 134,138,116 bp
  • A to G, chromosome 5 at 138,636,252 bp
  • G to A, chromosome 5 at 146,556,597 bp
  • A to T, chromosome 6 at 17,571,800 bp
  • A to G, chromosome 6 at 18,868,008 bp
  • A to T, chromosome 6 at 41,121,540 bp
  • A to G, chromosome 6 at 48,029,167 bp
  • A to T, chromosome 6 at 54,630,554 bp
  • T to C, chromosome 6 at 119,929,999 bp
  • A to T, chromosome 6 at 122,072,233 bp
  • A to T, chromosome 6 at 141,940,061 bp
  • A to G, chromosome 7 at 5,012,910 bp
  • A to G, chromosome 7 at 16,273,130 bp
  • G to A, chromosome 7 at 19,136,544 bp
  • A to T, chromosome 7 at 34,285,144 bp
  • A to G, chromosome 7 at 38,194,960 bp
  • T to C, chromosome 7 at 43,299,937 bp
  • T to A, chromosome 7 at 81,574,547 bp
  • C to A, chromosome 7 at 82,603,799 bp
  • C to A, chromosome 7 at 99,471,855 bp
  • C to A, chromosome 7 at 102,605,000 bp
  • A to G, chromosome 7 at 123,192,123 bp
  • A to C, chromosome 7 at 130,623,289 bp
  • A to T, chromosome 8 at 11,004,974 bp
  • G to A, chromosome 8 at 18,741,119 bp
  • C to T, chromosome 8 at 88,729,569 bp
  • A to G, chromosome 8 at 106,132,221 bp
  • A to T, chromosome 8 at 110,603,246 bp
  • C to T, chromosome 8 at 124,334,286 bp
  • C to A, chromosome 9 at 8,922,601 bp
  • C to T, chromosome 9 at 15,262,439 bp
  • T to C, chromosome 9 at 21,206,238 bp
  • C to T, chromosome 9 at 30,902,550 bp
  • T to A, chromosome 9 at 41,991,745 bp
  • C to T, chromosome 9 at 67,879,103 bp
  • C to T, chromosome 9 at 92,490,790 bp
  • C to A, chromosome 10 at 50,618,077 bp
  • C to T, chromosome 10 at 60,331,728 bp
  • C to A, chromosome 10 at 75,832,692 bp
  • A to G, chromosome 10 at 120,102,886 bp
  • A to G, chromosome 11 at 6,342,619 bp
  • T to G, chromosome 11 at 55,498,631 bp
  • A to G, chromosome 11 at 69,299,853 bp
  • A to T, chromosome 11 at 69,429,447 bp
  • T to C, chromosome 11 at 86,255,423 bp
  • A to G, chromosome 11 at 96,035,051 bp
  • T to A, chromosome 11 at 103,620,900 bp
  • A to G, chromosome 11 at 106,813,767 bp
  • T to C, chromosome 11 at 115,185,076 bp
  • T to G, chromosome 12 at 70,988,476 bp
  • A to T, chromosome 12 at 80,706,375 bp
  • T to A, chromosome 12 at 82,396,271 bp
  • T to A, chromosome 13 at 55,418,488 bp
  • G to T, chromosome 13 at 98,053,867 bp
  • T to A, chromosome 13 at 100,300,386 bp
  • T to A, chromosome 13 at 104,479,519 bp
  • C to A, chromosome 14 at 6,301,873 bp
  • C to A, chromosome 14 at 26,606,943 bp
  • T to C, chromosome 14 at 30,102,407 bp
  • T to C, chromosome 14 at 31,293,725 bp
  • T to A, chromosome 14 at 34,414,802 bp
  • A to T, chromosome 14 at 50,947,899 bp
  • T to A, chromosome 14 at 51,418,796 bp
  • A to C, chromosome 14 at 51,455,091 bp
  • A to G, chromosome 14 at 117,435,820 bp
  • G to A, chromosome 15 at 67,113,662 bp
  • C to T, chromosome 15 at 78,994,126 bp
  • T to C, chromosome 15 at 99,780,872 bp
  • A to G, chromosome 15 at 101,678,020 bp
  • G to A, chromosome 15 at 102,954,751 bp
  • T to C, chromosome 15 at 103,035,014 bp
  • A to T, chromosome 16 at 37,017,197 bp
  • T to C, chromosome 16 at 55,967,090 bp
  • A to T, chromosome 16 at 90,727,031 bp
  • A to G, chromosome 16 at 91,495,299 bp
  • A to G, chromosome 16 at 95,295,750 bp
  • A to G, chromosome 17 at 30,673,840 bp
  • A to T, chromosome 17 at 30,736,147 bp
  • C to A, chromosome 17 at 34,710,128 bp
  • T to C, chromosome 17 at 46,723,113 bp
  • A to G, chromosome 17 at 54,276,627 bp
  • A to G, chromosome 17 at 56,099,370 bp
  • G to A, chromosome 17 at 74,359,298 bp
  • C to A, chromosome 17 at 80,434,119 bp
  • G to A, chromosome 18 at 80,450,110 bp
  • C to A, chromosome 19 at 4,865,333 bp
  • T to C, chromosome 19 at 6,985,488 bp
  • G to T, chromosome 19 at 7,358,404 bp
  • C to G, chromosome 19 at 8,621,805 bp
  • T to C, chromosome 19 at 10,671,809 bp
  • G to A, chromosome Y at 914,056 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8348 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
067732-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.