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Strain Name:
C57BL/6J-MtgxR8301Btlr/Mmmh
Stock Number:
067789-MU
Citation ID:
RRID:MMRRC_067789-MU
Other Names:
R8301 (G1)
Major Collection:

Strain Information

Ighm
Name: immunoglobulin heavy constant mu
Synonyms: Ig mu, Igh6, Igh-M, muH, IgM
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 16019
HGNC: HGNC:5541
Sf1
Name: splicing factor 1
Synonyms: WBP4, MZFM, CW17R, Zfp162
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 22668
Homologene: 134065
Slc12a5
Name: solute carrier family 12, member 5
Synonyms: KCC2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 57138
Homologene: 10665
Runx1
Name: runt related transcription factor 1
Synonyms: AML1, Pebp2a2, runt domain, alpha subunit 2, Cbfa2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12394
Homologene: 1331
Ebf2
Name: early B cell factor 2
Synonyms: O/E-3, D14Ggc1e, Mmot1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 13592
Homologene: 56471
Ints6
Name: integrator complex subunit 6
Synonyms: Notch2l, 2900075H24Rik, DICE1, Ddx26
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18130
VEGA: 14
Homologene: 8121
Phf14
Name: PHD finger protein 14
Synonyms: 1110001C23Rik, 4932409F11Rik, 5730446A07Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 75725
Homologene: 8775
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 10,179,833 bp
  • G to T, chromosome 1 at 51,181,837 bp
  • T to G, chromosome 1 at 133,264,687 bp
  • T to A, chromosome 1 at 136,073,441 bp
  • G to A, chromosome 1 at 194,790,175 bp
  • C to T, chromosome 2 at 29,145,690 bp
  • T to C, chromosome 2 at 52,288,835 bp
  • A to T, chromosome 2 at 156,134,493 bp
  • A to T, chromosome 2 at 164,993,691 bp
  • A to G, chromosome 2 at 172,356,930 bp
  • T to C, chromosome 4 at 11,246,120 bp
  • A to G, chromosome 4 at 11,795,659 bp
  • A to G, chromosome 4 at 43,263,568 bp
  • T to C, chromosome 4 at 63,363,026 bp
  • A to T, chromosome 4 at 63,860,878 bp
  • A to T, chromosome 4 at 108,172,909 bp
  • A to G, chromosome 4 at 118,593,074 bp
  • T to C, chromosome 4 at 134,551,414 bp
  • A to G, chromosome 4 at 148,946,043 bp
  • A to T, chromosome 5 at 87,972,505 bp
  • T to G, chromosome 5 at 97,977,679 bp
  • C to T, chromosome 5 at 120,459,519 bp
  • G to C, chromosome 5 at 136,697,971 bp
  • G to C, chromosome 6 at 11,992,062 bp
  • A to G, chromosome 6 at 53,681,033 bp
  • A to G, chromosome 6 at 124,405,183 bp
  • A to T, chromosome 7 at 18,353,377 bp
  • C to T, chromosome 7 at 45,141,982 bp
  • A to T, chromosome 7 at 85,858,302 bp
  • T to C, chromosome 7 at 97,661,925 bp
  • G to T, chromosome 7 at 99,625,567 bp
  • T to C, chromosome 7 at 100,879,659 bp
  • T to G, chromosome 7 at 103,173,073 bp
  • A to G, chromosome 7 at 107,903,626 bp
  • T to C, chromosome 7 at 133,719,204 bp
  • T to A, chromosome 8 at 14,823,577 bp
  • A to G, chromosome 8 at 62,000,597 bp
  • T to C, chromosome 9 at 24,485,111 bp
  • T to G, chromosome 9 at 27,334,739 bp
  • T to A, chromosome 9 at 59,668,631 bp
  • T to A, chromosome 9 at 77,544,488 bp
  • T to G, chromosome 9 at 107,852,794 bp
  • G to A, chromosome 10 at 41,424,716 bp
  • A to G, chromosome 10 at 57,505,346 bp
  • C to A, chromosome 10 at 82,060,879 bp
  • T to A, chromosome 10 at 114,487,006 bp
  • T to C, chromosome 10 at 129,776,840 bp
  • T to C, chromosome 11 at 20,332,286 bp
  • T to A, chromosome 11 at 119,434,742 bp
  • C to T, chromosome 12 at 73,702,764 bp
  • A to G, chromosome 12 at 100,205,685 bp
  • C to T, chromosome 12 at 113,421,545 bp
  • A to C, chromosome 13 at 27,199,185 bp
  • A to C, chromosome 13 at 27,602,772 bp
  • A to G, chromosome 13 at 95,682,151 bp
  • A to G, chromosome 14 at 47,016,678 bp
  • T to A, chromosome 14 at 50,453,564 bp
  • A to G, chromosome 14 at 62,702,453 bp
  • A to C, chromosome 14 at 65,076,284 bp
  • A to G, chromosome 14 at 67,238,982 bp
  • A to G, chromosome 15 at 54,851,407 bp
  • T to C, chromosome 15 at 79,035,889 bp
  • G to T, chromosome 16 at 34,357,100 bp
  • C to A, chromosome 16 at 37,061,819 bp
  • A to G, chromosome 16 at 44,157,131 bp
  • A to C, chromosome 16 at 64,478,991 bp
  • C to A, chromosome 16 at 92,605,656 bp
  • T to C, chromosome 17 at 23,993,981 bp
  • A to T, chromosome 17 at 28,059,580 bp
  • A to G, chromosome 17 at 29,526,492 bp
  • T to C, chromosome 17 at 55,687,895 bp
  • C to T, chromosome 19 at 6,368,366 bp
  • T to C, chromosome 19 at 46,321,102 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8301 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067789-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.