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Strain Name:
C57BL/6J-MtgxR8345Btlr/Mmmh
Stock Number:
067801-MU
Citation ID:
RRID:MMRRC_067801-MU
Other Names:
R8345 (G1)
Major Collection:

Strain Information

Hapln1
Name: hyaluronan and proteoglycan link protein 1
Synonyms: link protein, cartilage linking protein 1, Crtl1l, Crtl1, CLP, LP-1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 12950
VEGA: 13
HGNC: HGNC:2380
Homologene: 1420
Dnmt3a
Name: DNA methyltransferase 3A
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 13435
HGNC: HGNC:2978
Homologene: 7294
Slc12a8
Name: solute carrier family 12 (potassium/chloride transporters), member 8
Synonyms: E330020C02Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 171286
Homologene: 11628
Scn3a
Name: sodium channel, voltage-gated, type III, alpha
Synonyms: LOC381367, Nav1.3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20269
Homologene: 56005
Prpf6
Name: pre-mRNA splicing factor 6
Synonyms: 1190003A07Rik, U5-102K, ANT-1, 2610031L17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 68879
Homologene: 5368
Jak2
Name: Janus kinase 2
Synonyms: C81284
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 16452
VEGA: 19
HGNC: HGNC:6192
Homologene: 21033
Sema4b
Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B
Synonyms: SemC, Semac
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20352
Homologene: 8426
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 11,199,894 bp
  • A to G, chromosome 1 at 36,348,816 bp
  • C to T, chromosome 1 at 74,928,044 bp
  • T to G, chromosome 1 at 139,464,273 bp
  • A to T, chromosome 1 at 185,455,467 bp
  • A to G, chromosome 2 at 65,498,991 bp
  • G to A, chromosome 2 at 66,494,622 bp
  • C to T, chromosome 2 at 85,062,882 bp
  • A to T, chromosome 2 at 86,315,107 bp
  • A to T, chromosome 2 at 87,532,347 bp
  • A to G, chromosome 2 at 88,592,091 bp
  • A to T, chromosome 2 at 90,252,217 bp
  • T to A, chromosome 2 at 111,527,519 bp
  • T to C, chromosome 2 at 112,652,925 bp
  • T to C, chromosome 2 at 118,240,471 bp
  • G to A, chromosome 2 at 118,810,803 bp
  • T to A, chromosome 2 at 131,078,578 bp
  • T to C, chromosome 2 at 181,650,158 bp
  • A to T, chromosome 3 at 135,295,252 bp
  • G to T, chromosome 3 at 146,507,676 bp
  • A to T, chromosome 3 at 157,546,094 bp
  • A to C, chromosome 4 at 43,291,714 bp
  • T to C, chromosome 4 at 43,483,168 bp
  • T to A, chromosome 4 at 126,376,928 bp
  • C to T, chromosome 4 at 143,416,868 bp
  • A to G, chromosome 5 at 108,125,957 bp
  • T to C, chromosome 5 at 123,786,930 bp
  • G to T, chromosome 6 at 15,799,654 bp
  • A to T, chromosome 6 at 125,679,302 bp
  • T to A, chromosome 6 at 129,325,630 bp
  • A to T, chromosome 7 at 6,008,257 bp
  • A to G, chromosome 7 at 8,253,602 bp
  • G to A, chromosome 7 at 28,155,360 bp
  • A to G, chromosome 7 at 30,855,364 bp
  • A to T, chromosome 7 at 41,427,544 bp
  • T to A, chromosome 7 at 80,220,819 bp
  • T to A, chromosome 7 at 83,942,165 bp
  • A to T, chromosome 7 at 88,074,538 bp
  • T to A, chromosome 7 at 104,564,224 bp
  • T to C, chromosome 7 at 109,905,270 bp
  • C to T, chromosome 7 at 119,442,787 bp
  • C to T, chromosome 7 at 127,883,910 bp
  • G to A, chromosome 8 at 77,689,184 bp
  • T to C, chromosome 8 at 122,781,545 bp
  • C to T, chromosome 8 at 127,324,068 bp
  • C to T, chromosome 8 at 128,798,764 bp
  • C to A, chromosome 9 at 15,999,274 bp
  • A to G, chromosome 9 at 18,444,483 bp
  • T to A, chromosome 9 at 23,224,830 bp
  • T to C, chromosome 9 at 72,617,691 bp
  • G to A, chromosome 9 at 73,005,719 bp
  • A to G, chromosome 10 at 128,671,331 bp
  • A to G, chromosome 11 at 50,149,832 bp
  • C to T, chromosome 11 at 51,743,778 bp
  • A to T, chromosome 11 at 53,684,141 bp
  • A to T, chromosome 11 at 65,187,132 bp
  • T to A, chromosome 11 at 69,107,556 bp
  • A to G, chromosome 11 at 76,210,779 bp
  • A to G, chromosome 11 at 101,164,279 bp
  • A to G, chromosome 11 at 103,186,614 bp
  • G to T, chromosome 11 at 120,377,030 bp
  • G to T, chromosome 11 at 120,784,075 bp
  • T to C, chromosome 12 at 3,835,234 bp
  • T to C, chromosome 12 at 8,709,454 bp
  • G to C, chromosome 12 at 87,201,438 bp
  • G to A, chromosome 13 at 34,948,181 bp
  • T to A, chromosome 13 at 46,673,578 bp
  • T to C, chromosome 13 at 49,520,800 bp
  • T to C, chromosome 13 at 81,103,386 bp
  • A to T, chromosome 13 at 89,584,783 bp
  • C to A, chromosome 14 at 31,264,594 bp
  • G to A, chromosome 14 at 86,829,093 bp
  • T to C, chromosome 15 at 4,944,326 bp
  • T to A, chromosome 15 at 76,601,490 bp
  • C to A, chromosome 16 at 33,550,951 bp
  • T to A, chromosome 17 at 17,712,865 bp
  • G to A, chromosome 18 at 34,634,097 bp
  • C to T, chromosome 18 at 58,058,431 bp
  • T to C, chromosome 19 at 29,284,870 bp
  • C to A, chromosome Y at 2,107,096 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8345 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067801-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.