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Strain Name:
C57BL/6J-MtgxR8445Btlr/Mmmh
Stock Number:
067886-MU
Citation ID:
RRID:MMRRC_067886-MU
Other Names:
R8445 (G1)
Major Collection:

Strain Information

Shprh
Name: SNF2 histone linker PHD RING helicase
Synonyms: 2610103K11Rik, D230017O13Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 268281
Homologene: 6489
Map4k5
Name: mitogen-activated protein kinase kinase kinase kinase 5
Synonyms: MAPKKKK5, GCKR, KHS, 4432415E19Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 399510
HGNC: HGNC:6867
Homologene: 38199
Smarca4
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Synonyms: SNF2beta, Brg1, SW1/SNF, b2b692Clo, b2b508.1Clo
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20586
Homologene: 135927
Mc3r
Name: melanocortin 3 receptor
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17201
HGNC: HGNC:6931
Homologene: 7412
Col3a1
Name: collagen, type III, alpha 1
Synonyms: Col3a-1, Tsk-2, Tsk2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12825
HGNC: HGNC:2201
Homologene: 55433
Thbs4
Name: thrombospondin 4
Synonyms: TSP-4, TSP4
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 21828
Homologene: 20691
Rbpms2
Name: RNA binding protein with multiple splicing 2
Synonyms: 2400008B06Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 71973
VEGA: 9
Homologene: 49895
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 45,341,180 bp
  • T to A, chromosome 1 at 63,647,514 bp
  • G to A, chromosome 1 at 134,040,998 bp
  • A to G, chromosome 2 at 32,608,520 bp
  • A to G, chromosome 2 at 79,281,781 bp
  • C to A, chromosome 2 at 104,681,933 bp
  • A to G, chromosome 2 at 172,249,317 bp
  • T to C, chromosome 3 at 87,813,584 bp
  • C to T, chromosome 3 at 116,007,346 bp
  • T to C, chromosome 3 at 127,586,205 bp
  • C to A, chromosome 4 at 22,385,983 bp
  • T to A, chromosome 4 at 59,720,526 bp
  • T to A, chromosome 4 at 96,133,022 bp
  • T to A, chromosome 4 at 115,525,594 bp
  • T to C, chromosome 4 at 136,932,289 bp
  • T to C, chromosome 5 at 120,973,370 bp
  • A to G, chromosome 6 at 43,167,297 bp
  • A to G, chromosome 6 at 124,026,036 bp
  • A to G, chromosome 6 at 125,134,604 bp
  • A to T, chromosome 6 at 130,227,115 bp
  • G to A, chromosome 7 at 3,620,929 bp
  • A to T, chromosome 7 at 4,836,353 bp
  • A to T, chromosome 7 at 23,340,540 bp
  • A to T, chromosome 7 at 55,979,718 bp
  • G to A, chromosome 7 at 75,730,465 bp
  • A to C, chromosome 7 at 118,136,977 bp
  • T to A, chromosome 7 at 131,090,380 bp
  • T to A, chromosome 9 at 20,171,098 bp
  • CGAGGAGGAGGAGGAGG to CGAGGAGGAGGAGG, chromosome 9 at 21,700,943 bp
  • C to T, chromosome 9 at 65,651,021 bp
  • T to G, chromosome 9 at 70,766,921 bp
  • C to T, chromosome 10 at 11,181,569 bp
  • C to T, chromosome 10 at 34,297,746 bp
  • T to C, chromosome 10 at 77,910,252 bp
  • A to T, chromosome 10 at 130,477,466 bp
  • A to G, chromosome 11 at 50,833,386 bp
  • T to A, chromosome 12 at 69,850,967 bp
  • T to C, chromosome 12 at 75,632,862 bp
  • T to C, chromosome 13 at 28,021,462 bp
  • G to T, chromosome 13 at 65,033,177 bp
  • C to A, chromosome 13 at 92,790,841 bp
  • A to C, chromosome 14 at 55,826,418 bp
  • C to A, chromosome 15 at 75,947,553 bp
  • C to T, chromosome 15 at 88,814,486 bp
  • C to A, chromosome 16 at 49,120,018 bp
  • T to G, chromosome 17 at 33,003,578 bp
  • T to C, chromosome 18 at 34,768,315 bp
  • G to A, chromosome 18 at 56,957,890 bp
  • A to G, chromosome 18 at 67,473,778 bp
  • T to A, chromosome 19 at 45,234,212 bp
  • A to G, chromosome Y at 916,874 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8445 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067886-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.