Strain Name:
C57BL/6J-MtgxR8539Btlr/Mmmh
Stock Number:
068505-MU
Citation ID:
RRID:MMRRC_068505-MU
Other Names:
R8539 (G1)
Major Collection:

Strain Information

Suz12
Name: SUZ12 polycomb repressive complex 2 subunit
Synonyms: D11Ertd530e, 2610028O16Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 52615
Homologene: 32256
Notch3
Name: notch 3
Synonyms: N3, hpbk
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18131
HGNC: HGNC:7883
Homologene: 376
Plxnd1
Name: plexin D1
Synonyms: 6230425C21Rik, b2b1863Clo, b2b553Clo
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67784
HGNC: HGNC:9107
Homologene: 22866
Igf1r
Name: insulin-like growth factor I receptor
Synonyms: IGF-1R, A330103N21Rik, hyft, CD221, line 186
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16001
HGNC: HGNC:5465
Homologene: 30997
Zeb1
Name: zinc finger E-box binding homeobox 1
Synonyms: Tw, Zfhx1a, Zfx1a, Zfhep, Tcf18, MEB1, Nil2, 3110032K11Rik, Tcf8, AREB6, ZEB, [delta]EF1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 21417
Homologene: 31779
Sorbs1
Name: sorbin and SH3 domain containing 1
Synonyms: Sh3d5, Ponsin, c-Cbl-associated protein, CAP, 9530001P15Rik, 2310065E01Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20411
VEGA: 19
Homologene: 83252
Hnf1a
Name: HNF1 homeobox A
Synonyms: hepatocyte nuclear factor 1, Hnf1alpha, LFB1, Tcf1, HNF1, Hnf-1, HNF1[a], HNF1-alpha
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 21405
Homologene: 459
Cntrob
Name: centrobin, centrosomal BRCA2 interacting protein
Synonyms: Nip2, 9830165K03Rik, Lip8
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216846
Homologene: 14205
Ncapd3
Name: non-SMC condensin II complex, subunit D3
Synonyms: 2810487N22Rik, B130055D15Rik, 4632407J06Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 78658
VEGA: 9
Homologene: 41021
Scn10a
Name: sodium channel, voltage-gated, type X, alpha
Synonyms: Nav1.8
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20264
VEGA: 9
Homologene: 21300
Ror1
Name: receptor tyrosine kinase-like orphan receptor 1
Synonyms: Ntrkr1, 2810404D04Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 26563
Homologene: 3675
Tap1
Name: transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)
Synonyms: Ham1, Ham-1, Tap-1, RING4, Abcb2, PSF-1, MTP1, TAP
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 21354
HGNC: HGNC:43
Homologene: 495
Tlr3
Name: toll-like receptor 3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 142980
Homologene: 20696
Hcrt
Name: hypocretin
Synonyms: orexin B, orexin A
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15171
HGNC: HGNC:4847
Homologene: 1166
Klhl8
Name: kelch-like 8
Synonyms: 2310001P09Rik, D5Ertd431e
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 246293
Homologene: 10819
Ret
Name: ret proto-oncogene
Synonyms: c-Ret, RET51, RET9
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 19713
HGNC: HGNC:9967
Homologene: 7517
Myo7a
Name: myosin VIIA
Synonyms: nmf371, polka, Myo7, Hdb, USH1B
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 17921
HGNC: HGNC:7606
Homologene: 219
Tnik
Name: TRAF2 and NCK interacting kinase
Synonyms: 4831440I19Rik, C530008O15Rik, 1500031A17Rik, C630040K21Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 665113
Homologene: 77943
Ttn
Name: titin
Synonyms: shru, L56, mdm, connectin, D330041I19Rik, 2310057K23Rik, 2310074I15Rik, 2310036G12Rik, D830007G01Rik, 1100001C23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Pkd1l3
Name: polycystic kidney disease 1 like 3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244646
Homologene: 14627
Aplnr
Name: apelin receptor
Synonyms: msr/apj, apelin receptor, APJ, Agtrl1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 23796
HGNC: HGNC:339
Homologene: 3784
Vmn2r111
Name: vomeronasal 2, receptor 111
Synonyms: EG210876
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 210876
Homologene: 86604
Myom2
Name: myomesin 2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 17930
HGNC: HGNC:7614
Homologene: 2953
Hykk
Name: hydroxylysine kinase 1
Synonyms: Agphd1, C630028N24Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235386
Homologene: 16057
Galnt13
Name: polypeptide N-acetylgalactosaminyltransferase 13
Synonyms: pp-GalNAc-T13
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 271786
Homologene: 62167
Sema5a
Name: sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A
Synonyms: 9130201M22Rik, semF, M-Sema D, Semaf
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20356
VEGA: 15
Homologene: 2949
Loxl3
Name: lysyl oxidase-like 3
Synonyms: Lor2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16950
Homologene: 56591
Or2p2
Name: olfactory receptor family 2 subfamily P member 2
Synonyms: GA_x6K02T2QHY8-12181473-12182423, MOR256-14, Olfr1370
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 258528
Homologene: 105231
Ndufaf5
Name: NADH:ubiquinone oxidoreductase complex assembly factor 5
Synonyms: 2310003L22Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 69487
Homologene: 11474
Sult1b1
Name: sulfotransferase family 1B, member 1
Synonyms: Dopa/tyrosine sulfotransferase
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56362
Homologene: 69169
Vmn2r106
Name: vomeronasal 2, receptor 106
Synonyms: EG224576
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224576
Homologene: 135824
Usp21
Name: ubiquitin specific peptidase 21
Synonyms: W53272, ESTM28, Usp16, Usp23
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 30941
Homologene: 56572
Ephb4
Name: Eph receptor B4
Synonyms: Tyro11, b2b2412Clo, MDK2, Htk, Myk1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13846
HGNC: HGNC:3395
Homologene: 20939
Akr1c6
Name: aldo-keto reductase family 1, member C6
Synonyms: 3alpha-HSD, Hsd17b5, Akr1c1, estradiol 17-beta-dehydrogenase (A-specific)
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 83702
Homologene: 84695
Nudt21
Name: nudix hydrolase 21
Synonyms: 3110048P04Rik, nudix (nucleoside diphosphate linked moiety X)-type motif 21, Cpsf5, 5730530J16Rik, 25kDa
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 68219
Homologene: 5090
Amz1
Name: archaelysin family metallopeptidase 1
Synonyms: 6530401C20Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231842
Homologene: 18290
Vmn1r122
Name: vomeronasal 1 receptor 122
Synonyms: Gm5729
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 435951
Homologene: 104166
Arpc5
Name: actin related protein 2/3 complex, subunit 5
Synonyms: p16-Arc, 5830443F10Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 67771
HGNC: HGNC:708
Homologene: 4176
Eppk1
Name: epiplakin 1
Synonyms: EPPK, EPIPL1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223650
VEGA: 15
Homologene: 20006
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 152,766,801 bp
  • C to T, chromosome 1 at 171,283,673 bp
  • A to T, chromosome 2 at 54,933,572 bp
  • A to G, chromosome 2 at 76,906,434 bp
  • A to G, chromosome 2 at 85,136,907 bp
  • C to A, chromosome 2 at 140,183,974 bp
  • G to A, chromosome 3 at 28,542,003 bp
  • T to C, chromosome 4 at 100,441,887 bp
  • C to A, chromosome 5 at 87,533,979 bp
  • C to T, chromosome 5 at 103,867,526 bp
  • A to G, chromosome 5 at 114,970,517 bp
  • A to G, chromosome 5 at 137,357,855 bp
  • T to C, chromosome 5 at 140,748,657 bp
  • A to T, chromosome 6 at 83,049,526 bp
  • G to C, chromosome 6 at 115,962,807 bp
  • A to G, chromosome 6 at 118,175,809 bp
  • A to T, chromosome 7 at 21,133,356 bp
  • C to A, chromosome 7 at 68,003,848 bp
  • G to A, chromosome 7 at 98,072,461 bp
  • A to T, chromosome 8 at 15,114,254 bp
  • A to G, chromosome 8 at 45,398,516 bp
  • G to A, chromosome 8 at 94,036,973 bp
  • T to A, chromosome 8 at 109,636,287 bp
  • T to G, chromosome 9 at 27,048,224 bp
  • A to G, chromosome 9 at 54,937,160 bp
  • T to A, chromosome 9 at 119,638,774 bp
  • G to A, chromosome 11 at 69,320,826 bp
  • T to A, chromosome 11 at 79,999,078 bp
  • T to A, chromosome 11 at 100,761,225 bp
  • G to A, chromosome 13 at 4,434,475 bp
  • T to C, chromosome 13 at 21,073,173 bp
  • T to A, chromosome 15 at 32,618,843 bp
  • C to T, chromosome 15 at 76,110,119 bp
  • C to A, chromosome 17 at 20,279,009 bp
  • A to T, chromosome 17 at 22,571,293 bp
  • A to T, chromosome 17 at 32,156,355 bp
  • C to T, chromosome 17 at 34,189,435 bp
  • T to A, chromosome 18 at 5,748,784 bp
  • G to C, chromosome 19 at 40,376,800 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8539 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068505-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.