Strain Name:
C57BL/6J-MtgxR8546Btlr/Mmmh
Stock Number:
068511-MU
Citation ID:
RRID:MMRRC_068511-MU
Other Names:
R8546 (G1)
Major Collection:

Strain Information

Col3a1
Name: collagen, type III, alpha 1
Synonyms: Col3a-1, Tsk-2, Tsk2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12825
HGNC: HGNC:2201
Homologene: 55433
Ncam2
Name: neural cell adhesion molecule 2
Synonyms: RNCAM, R4B12 antigen, Ncam-2, Ocam
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 17968
HGNC: HGNC:7657
Homologene: 3336
Adcy9
Name: adenylate cyclase 9
Synonyms: D16Wsu65e, ACtp10
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 11515
HGNC: HGNC:240
Homologene: 868
Kmt2e
Name: lysine (K)-specific methyltransferase 2E
Synonyms: 1810033J14Rik, D230038D11Rik, 9530077A04Rik, Mll5
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 69188
Homologene: 18822
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Lin7c
Name: lin-7 homolog C, crumbs cell polarity complex component
Synonyms: LIN-7-C, LIN-7C, Lin7c, MALS-3, Veli3, D2Ertd520e, 9130007B12Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22343
Homologene: 22649
Patj
Name: PATJ, crumbs cell polarity complex component
Synonyms: Cipp, Inadl
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12695
Homologene: 72199
Cabin1
Name: calcineurin binding protein 1
Synonyms: Ppp3in, Cain, A330070M20Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 104248
VEGA: 10
Homologene: 49307
Cyb5a
Name: cytochrome b5 type A (microsomal)
Synonyms: 0610009N12Rik, Cyb5
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 109672
HGNC: HGNC:2570
Homologene: 41475
Bltp3b
Name: bridge-like lipid transfer protein family member 3B
Synonyms: 4930506D01Rik, 2010319N22Rik, E030041M21Rik, Uhrf1bp1l
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 75089
VEGA: 10
Homologene: 12590
Eif3a
Name: eukaryotic translation initiation factor 3, subunit A
Synonyms: Csma, Eif3, A830012B05Rik, Eif3s10
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 13669
VEGA: 19
HGNC: HGNC:3271
Homologene: 2779
Usp24
Name: ubiquitin specific peptidase 24
Synonyms: 2810030C21Rik, 2700066K03Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 329908
Homologene: 35420
Polr3f
Name: polymerase (RNA) III (DNA directed) polypeptide F
Synonyms: 3110032A07Rik, 2810411G20Rik, RPC39, RPC6, 3010019O03Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 70408
Homologene: 4722
Fam171b
Name: family with sequence similarity 171, member B
Synonyms: D430039N05Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241520
Homologene: 18462
Mcm6
Name: minichromosome maintenance complex component 6
Synonyms: Mcmd6, D1Wsu22e
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17219
HGNC: HGNC:6949
Homologene: 4322
Ythdc1
Name: YTH domain containing 1
Synonyms: A730098D12Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231386
Homologene: 15796
Fnip1
Name: folliculin interacting protein 1
Synonyms: A730024A03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216742
Homologene: 28173
Skor1
Name: SKI family transcriptional corepressor 1
Synonyms: C230094B15Rik, Corl1, Lbxcor1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 207667
Homologene: 18175
Pkd1l3
Name: polycystic kidney disease 1 like 3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244646
Homologene: 14627
Ctu2
Name: cytosolic thiouridylase subunit 2
Synonyms: 2310061F22Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 66965
Homologene: 79815
Dnah17
Name: dynein, axonemal, heavy chain 17
Synonyms: LOC382552, 2810003K23Rik, Dnahcl1, Dnahc17
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 69926
HGNC: HGNC:2946
Homologene: 72102
Lonp1
Name: lon peptidase 1, mitochondrial
Synonyms: LON, 1200017E13Rik, Prss15
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 74142
VEGA: 17
HGNC: HGNC:9479
Homologene: 3521
Prmt6
Name: protein arginine N-methyltransferase 6
Synonyms: Hrmt1l6
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99890
Homologene: 10024
Myo7b
Name: myosin VIIB
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 17922
HGNC: HGNC:7607
Homologene: 81947
Or7e169
Name: olfactory receptor family 7 subfamily E member 169
Synonyms: GA_x6K02T2PVTD-13586614-13585661, MOR146-2, Olfr860
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258521
Homologene: 134093
Fras1
Name: Fraser extracellular matrix complex subunit 1
Synonyms: bl, E130113P14Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231470
Homologene: 23516
Bsn
Name: bassoon
Synonyms: presynaptic cytomatrix protein
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12217
HGNC: HGNC:1117
Homologene: 31161
Mettl2
Name: methyltransferase 2, methylcytidine
Synonyms: C130031G21Rik, PSENIP1, D11Ertd768e, 2810438F06Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 52686
Homologene: 10174
Rgl3
Name: ral guanine nucleotide dissociation stimulator-like 3
Synonyms: 1300003D20Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 71746
VEGA: 9
Homologene: 11363
Slc28a2b
Name: solute carrier family 28 member 2b
Synonyms: Gm14085
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 381417
Gjc2
Name: gap junction protein, gamma 2
Synonyms: connexin 47, Cx47, B230382L12Rik, Gja12
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 118454
Homologene: 10715
Acot1
Name: acyl-CoA thioesterase 1
Synonyms: D12Ucla1, CTE-1, ACH2, CTE-I, Cte1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 26897
VEGA: 12
Homologene: 22686
Axl
Name: AXL receptor tyrosine kinase
Synonyms: Tyro7, Ark, Ufo
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 26362
HGNC: HGNC:905
Homologene: 7583
Igsf9b
Name: immunoglobulin superfamily, member 9B
Synonyms: LOC235086, AI414108
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235086
Homologene: 19472
Rhoj
Name: ras homolog family member J
Synonyms: TCL, 1110005O19Rik, TC10L, Arhj
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 80837
HGNC: HGNC:688
Homologene: 56894
Atp11a
Name: ATPase, class VI, type 11A
Synonyms: Ih, 4930558F19Rik, 9130422H11Rik, LOC100045280
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 50770
Homologene: 75050
Cdh20
Name: cadherin 20
Synonyms: Cdh7
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 23836
HGNC: HGNC:1760
Homologene: 8015
Tnni3k
Name: TNNI3 interacting kinase
Synonyms: Cark, D830019J24Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 435766
Homologene: 41084
Clca3b
Name: chloride channel accessory 3B
Synonyms: Clca4
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229927
HGNC: HGNC:2017
Homologene: 135610
Irx6
Name: Iroquois homeobox 6
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 64379
Homologene: 41481
Poll
Name: polymerase (DNA directed), lambda
Synonyms: 1110003P06Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 56626
VEGA: 19
HGNC: HGNC:9184
Homologene: 40863
Khnyn
Name: KH and NYN domain containing
Synonyms: 9130227C08Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 219094
VEGA: 14
Homologene: 23611
Tlr1
Name: toll-like receptor 1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 21897
Homologene: 20694
Padi4
Name: peptidyl arginine deiminase, type IV
Synonyms: PAD type IV, Pdi4, Pad4
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18602
Homologene: 7883
H2bc13
Name: H2B clustered histone 13
Synonyms: Hist1h2bl
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 319185
Homologene: 136773
Fahd1
Name: fumarylacetoacetate hydrolase domain containing 1
Synonyms: 1110025H10Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 68636
VEGA: 17
Homologene: 6774
Tekt5
Name: tektin 5
Synonyms: 3300001K11Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 70426
Homologene: 71928
Tulp1
Name: TUB like protein 1
Synonyms: Tulp1l
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 22157
Homologene: 2491
Loxl4
Name: lysyl oxidase-like 4
Synonyms: 4833426I20Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 67573
Homologene: 12977
Cyb5rl
Name: cytochrome b5 reductase-like
Synonyms: 2810410C14Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230582
Homologene: 45506
Cfap97d1
Name: CFAP97 domain containing 1
Synonyms: 1700006E09Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 75437
Homologene: 19137
Eif6
Name: eukaryotic translation initiation factor 6
Synonyms: p27BBP, eIF6, imc-415, Itgb4bp
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16418
HGNC: HGNC:6159
Homologene: 7135
Tuft1
Name: tuftelin 1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 22156
Homologene: 7985
Zscan4-ps3
Name: zinc finger and SCAN domain containing 4, pseudogene 3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 100043042
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 45,340,939 bp
  • A to G, chromosome 1 at 104,934,044 bp
  • C to T, chromosome 1 at 128,345,948 bp
  • A to G, chromosome 2 at 83,855,451 bp
  • A to G, chromosome 2 at 109,896,371 bp
  • A to T, chromosome 2 at 122,522,754 bp
  • A to G, chromosome 2 at 144,532,364 bp
  • A to G, chromosome 2 at 155,826,500 bp
  • T to A, chromosome 3 at 94,622,113 bp
  • C to T, chromosome 3 at 110,250,718 bp
  • T to A, chromosome 3 at 144,827,397 bp
  • G to T, chromosome 3 at 154,792,807 bp
  • T to A, chromosome 4 at 98,437,397 bp
  • G to A, chromosome 4 at 106,402,129 bp
  • T to A, chromosome 4 at 107,068,726 bp
  • T to C, chromosome 4 at 140,757,530 bp
  • A to T, chromosome 5 at 23,481,244 bp
  • T to A, chromosome 5 at 64,927,031 bp
  • A to T, chromosome 5 at 86,826,748 bp
  • G to A, chromosome 5 at 96,709,966 bp
  • T to A, chromosome 7 at 11,612,770 bp
  • C to T, chromosome 7 at 25,774,163 bp
  • T to A, chromosome 8 at 12,851,083 bp
  • A to T, chromosome 8 at 92,678,636 bp
  • G to A, chromosome 8 at 109,666,983 bp
  • T to C, chromosome 8 at 122,479,363 bp
  • T to A, chromosome 9 at 19,846,389 bp
  • T to C, chromosome 9 at 21,987,860 bp
  • T to C, chromosome 9 at 27,333,130 bp
  • T to C, chromosome 9 at 63,142,288 bp
  • G to A, chromosome 9 at 108,111,452 bp
  • T to C, chromosome 10 at 75,742,267 bp
  • A to G, chromosome 10 at 89,794,535 bp
  • A to G, chromosome 11 at 54,510,000 bp
  • G to T, chromosome 11 at 59,176,356 bp
  • A to G, chromosome 11 at 101,990,861 bp
  • T to A, chromosome 11 at 105,131,573 bp
  • A to G, chromosome 11 at 118,124,275 bp
  • A to G, chromosome 12 at 75,375,350 bp
  • T to C, chromosome 12 at 84,017,365 bp
  • G to A, chromosome 13 at 21,715,872 bp
  • A to G, chromosome 14 at 55,885,818 bp
  • G to T, chromosome 16 at 4,418,905 bp
  • A to G, chromosome 16 at 10,358,013 bp
  • A to G, chromosome 16 at 15,663,035 bp
  • C to T, chromosome 16 at 81,517,531 bp
  • T to C, chromosome 17 at 24,850,083 bp
  • T to C, chromosome 17 at 28,363,736 bp
  • G to A, chromosome 17 at 56,626,702 bp
  • T to C, chromosome 18 at 31,990,148 bp
  • G to A, chromosome 18 at 84,871,634 bp
  • T to C, chromosome 19 at 42,607,588 bp
  • T to A, chromosome 19 at 45,557,977 bp
  • A to T, chromosome 19 at 60,766,770 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8546 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068511-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.