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Strain Name:
C57BL/6J-MtgxR8802Btlr/Mmmh
Stock Number:
068639-MU
Citation ID:
RRID:MMRRC_068639-MU
Other Names:
R8802 (G1)
Major Collection:

Strain Information

Gdf6
Name: growth differentiation factor 6
Synonyms: BMP13
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242316
HGNC: HGNC:4221
Homologene: 40883
Utp20
Name: UTP20 small subunit processome component
Synonyms: mDRIM, DRIM, 3830408P06Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70683
VEGA: 10
Homologene: 38373
Bicral
Name: BRD4 interacting chromatin remodeling complex associated protein like
Synonyms: BC032203, Gltscr1l
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 210982
VEGA: 17
Homologene: 19366
Gcn1
Name: GCN1 activator of EIF2AK4
Synonyms: GCN1L, G431004K08Rik, Gcn1l1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231659
HGNC: HGNC:4199
Homologene: 5887
Skil
Name: SKI-like
Synonyms: SnoN, Skir, sno-dE3, SnoN2, 9130011J04Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20482
Homologene: 3948
Nampt
Name: nicotinamide phosphoribosyltransferase
Synonyms: 1110035O14Rik, Visfatin, Pbef1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 59027
VEGA: 12
Homologene: 4201
Rbbp6
Name: retinoblastoma binding protein 6, ubiquitin ligase
Synonyms: 4933422O15Rik, C030034J04Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 19647
HGNC: HGNC:9889
Homologene: 136812
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • CA to CAA, chromosome 1 at 65,165,188 bp
  • A to G, chromosome 1 at 174,137,050 bp
  • T to A, chromosome 2 at 31,411,276 bp
  • A to G, chromosome 2 at 97,630,258 bp
  • T to C, chromosome 2 at 119,034,897 bp
  • A to T, chromosome 2 at 150,935,252 bp
  • A to G, chromosome 2 at 157,196,153 bp
  • T to A, chromosome 2 at 163,397,264 bp
  • T to A, chromosome 3 at 20,019,906 bp
  • C to T, chromosome 3 at 31,113,443 bp
  • T to C, chromosome 3 at 83,346,237 bp
  • T to C, chromosome 3 at 84,952,386 bp
  • T to A, chromosome 3 at 88,378,261 bp
  • T to A, chromosome 3 at 89,092,233 bp
  • T to A, chromosome 3 at 103,645,370 bp
  • A to T, chromosome 3 at 106,404,892 bp
  • T to C, chromosome 3 at 152,615,994 bp
  • C to T, chromosome 4 at 9,844,769 bp
  • C to A, chromosome 4 at 88,755,010 bp
  • G to T, chromosome 5 at 21,925,259 bp
  • C to T, chromosome 5 at 30,173,833 bp
  • T to C, chromosome 5 at 115,609,883 bp
  • A to T, chromosome 5 at 145,030,066 bp
  • A to T, chromosome 6 at 69,943,406 bp
  • A to T, chromosome 7 at 5,411,081 bp
  • A to G, chromosome 7 at 12,515,488 bp
  • G to A, chromosome 7 at 15,833,847 bp
  • G to A, chromosome 7 at 30,584,071 bp
  • A to G, chromosome 7 at 46,008,859 bp
  • A to G, chromosome 7 at 102,071,332 bp
  • T to A, chromosome 7 at 122,988,457 bp
  • T to C, chromosome 8 at 3,457,308 bp
  • G to A, chromosome 9 at 3,635,050 bp
  • T to C, chromosome 9 at 96,685,570 bp
  • C to T, chromosome 9 at 111,020,950 bp
  • C to T, chromosome 10 at 14,139,422 bp
  • A to G, chromosome 10 at 60,409,098 bp
  • A to T, chromosome 10 at 79,478,427 bp
  • T to C, chromosome 10 at 88,747,295 bp
  • A to G, chromosome 11 at 55,282,924 bp
  • T to C, chromosome 11 at 70,555,998 bp
  • T to C, chromosome 11 at 117,775,075 bp
  • T to C, chromosome 11 at 119,462,102 bp
  • T to C, chromosome 11 at 121,424,987 bp
  • C to T, chromosome 12 at 8,728,726 bp
  • T to A, chromosome 12 at 28,526,721 bp
  • C to A, chromosome 12 at 32,850,436 bp
  • T to C, chromosome 12 at 55,738,316 bp
  • T to C, chromosome 12 at 85,327,418 bp
  • A to G, chromosome 13 at 23,905,291 bp
  • A to G, chromosome 13 at 33,029,604 bp
  • T to C, chromosome 15 at 30,966,876 bp
  • A to T, chromosome 16 at 11,319,955 bp
  • T to A, chromosome 17 at 21,026,605 bp
  • T to A, chromosome 17 at 23,309,862 bp
  • G to A, chromosome 17 at 24,905,232 bp
  • A to G, chromosome 17 at 46,824,700 bp
  • C to T, chromosome 17 at 73,918,410 bp
  • T to A, chromosome 18 at 5,213,417 bp
  • A to G, chromosome 18 at 36,954,158 bp
  • T to C, chromosome 18 at 71,826,054 bp
  • T to C, chromosome 19 at 6,047,938 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8802 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068639-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.