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Strain Name:
C57BL/6J-MtgxR8804Btlr/Mmmh
Stock Number:
068641-MU
Citation ID:
RRID:MMRRC_068641-MU
Other Names:
R8804 (G1)
Major Collection:

Strain Information

Pou2f1
Name: POU domain, class 2, transcription factor 1
Synonyms: Oct-1C, Oct-1B, Oct-1A, oct-1, Otf-1, Otf1, 2810482H01Rik, Oct-1z, Oct1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18986
HGNC: HGNC:9212
Homologene: 37658
Pitpnc1
Name: phosphatidylinositol transfer protein, cytoplasmic 1
Synonyms: C330017I21Rik, 5830436L09Rik, 1110020B03Rik, RDGB-BETA, RDGBB1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 71795
Homologene: 138301
Plaat1
Name: phospholipase A and acyltransferase 1
Synonyms: A-C1, 2810012B06Rik, Hrasls
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 27281
Homologene: 8452
Camsap3
Name: calmodulin regulated spectrin-associated protein family, member 3
Synonyms: Nezha, 2310057J16Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 69697
Homologene: 18966
Ephx2
Name: epoxide hydrolase 2, cytoplasmic
Synonyms: Eph2, sEP, sEH
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 13850
VEGA: 14
HGNC: HGNC:3402
Homologene: 37558
Stk11ip
Name: serine/threonine kinase 11 interacting protein
Synonyms: LKB1IP, LIP1, 1200014D22Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 71728
Homologene: 12406
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 44,056,649 bp
  • A to G, chromosome 1 at 75,535,256 bp
  • G to T, chromosome 1 at 153,753,915 bp
  • A to T, chromosome 1 at 159,858,312 bp
  • T to C, chromosome 1 at 165,880,470 bp
  • C to T, chromosome 1 at 171,276,374 bp
  • T to A, chromosome 2 at 30,330,879 bp
  • A to G, chromosome 2 at 31,425,381 bp
  • C to A, chromosome 2 at 156,051,994 bp
  • A to G, chromosome 2 at 158,547,706 bp
  • G to A, chromosome 2 at 175,169,859 bp
  • T to C, chromosome 3 at 19,628,656 bp
  • A to T, chromosome 3 at 28,594,053 bp
  • T to G, chromosome 3 at 59,336,455 bp
  • A to T, chromosome 3 at 93,395,843 bp
  • T to A, chromosome 3 at 99,967,190 bp
  • T to C, chromosome 3 at 144,839,137 bp
  • A to T, chromosome 3 at 148,847,016 bp
  • A to G, chromosome 4 at 58,206,043 bp
  • G to A, chromosome 4 at 119,468,076 bp
  • G to A, chromosome 4 at 126,750,105 bp
  • T to A, chromosome 4 at 130,005,419 bp
  • C to T, chromosome 4 at 147,913,321 bp
  • T to C, chromosome 5 at 124,001,512 bp
  • A to G, chromosome 5 at 139,231,165 bp
  • A to T, chromosome 6 at 23,496,806 bp
  • A to G, chromosome 6 at 54,273,076 bp
  • G to A, chromosome 6 at 73,065,773 bp
  • T to A, chromosome 6 at 87,016,432 bp
  • C to T, chromosome 6 at 91,987,526 bp
  • T to C, chromosome 6 at 129,465,555 bp
  • T to A, chromosome 6 at 133,055,363 bp
  • A to G, chromosome 7 at 6,390,400 bp
  • A to T, chromosome 7 at 18,682,659 bp
  • T to A, chromosome 7 at 44,757,846 bp
  • G to A, chromosome 7 at 45,520,974 bp
  • T to C, chromosome 7 at 130,692,963 bp
  • T to C, chromosome 7 at 141,269,407 bp
  • A to G, chromosome 8 at 3,602,624 bp
  • T to A, chromosome 8 at 61,958,606 bp
  • T to A, chromosome 8 at 83,319,521 bp
  • T to C, chromosome 8 at 85,359,245 bp
  • G to T, chromosome 9 at 64,235,324 bp
  • T to C, chromosome 9 at 111,264,904 bp
  • T to C, chromosome 10 at 83,572,151 bp
  • A to G, chromosome 11 at 16,869,339 bp
  • A to T, chromosome 11 at 35,883,317 bp
  • T to C, chromosome 11 at 68,991,082 bp
  • T to C, chromosome 11 at 69,465,685 bp
  • T to C, chromosome 11 at 71,784,335 bp
  • T to A, chromosome 11 at 83,016,813 bp
  • T to A, chromosome 11 at 107,212,605 bp
  • G to T, chromosome 11 at 113,873,152 bp
  • A to T, chromosome 12 at 24,668,611 bp
  • T to C, chromosome 12 at 112,658,607 bp
  • G to T, chromosome 12 at 114,879,961 bp
  • G to T, chromosome 13 at 23,903,262 bp
  • A to G, chromosome 13 at 46,687,159 bp
  • A to G, chromosome 14 at 55,074,734 bp
  • G to T, chromosome 14 at 56,616,443 bp
  • T to C, chromosome 14 at 64,750,342 bp
  • T to C, chromosome 14 at 66,087,020 bp
  • A to G, chromosome 14 at 70,123,847 bp
  • A to T, chromosome 14 at 121,605,183 bp
  • G to A, chromosome 15 at 55,792,127 bp
  • T to G, chromosome 15 at 76,124,084 bp
  • C to A, chromosome 15 at 76,519,211 bp
  • T to A, chromosome 16 at 20,725,957 bp
  • T to A, chromosome 16 at 29,220,453 bp
  • G to A, chromosome 16 at 58,461,049 bp
  • A to G, chromosome 16 at 85,802,412 bp
  • A to G, chromosome 16 at 85,869,912 bp
  • A to G, chromosome 17 at 26,216,557 bp
  • T to A, chromosome 17 at 34,200,251 bp
  • A to T, chromosome 17 at 35,032,854 bp
  • A to T, chromosome 17 at 74,047,634 bp
  • A to G, chromosome 18 at 52,694,536 bp
  • A to G, chromosome 19 at 7,014,326 bp
  • T to A, chromosome 19 at 33,964,798 bp
  • G to C, chromosome 19 at 46,585,804 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8804 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068641-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.