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Strain Name:
C57BL/6J-MtgxR8929Btlr/Mmmh
Stock Number:
068773-MU
Citation ID:
RRID:MMRRC_068773-MU
Other Names:
R8929 (G1)
Major Collection:

Strain Information

B4galt6
Name: UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 6
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 56386
VEGA: 18
HGNC: HGNC:929
Homologene: 3506
Cdh1
Name: cadherin 1
Synonyms: E-cadherin, Ecad, UM, uvomorulin, L-CAM, E-cad
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 12550
HGNC: HGNC:1748
Homologene: 20917
Sod2
Name: superoxide dismutase 2, mitochondrial
Synonyms: MnSOD, manganese SOD, manganese superoxide dismutase, Sod-2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20656
VEGA: 17
Homologene: 530
Hgf
Name: hepatocyte growth factor
Synonyms: scatter factor, NK1, SF/HGF, HGF/SF, NK2, C230052L06Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 15234
HGNC: HGNC:4893
Homologene: 503
Epyc
Name: epiphycan
Synonyms: PG-Lb, epiphycan, SLRR3B, Dspg3
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 13516
HGNC: HGNC:3053
Homologene: 3634
Lefty1
Name: left right determination factor 1
Synonyms: lefty-1, Stra3, Lefty, Ebaf
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13590
HGNC: HGNC:6552
Homologene: 49231
Pds5b
Name: PDS5 cohesin associated factor B
Synonyms: AS3, Aprin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100710
Homologene: 41001
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 119,667,548 bp
  • A to G, chromosome 1 at 180,937,725 bp
  • C to A, chromosome 2 at 17,393,180 bp
  • A to G, chromosome 2 at 30,110,869 bp
  • A to T, chromosome 2 at 76,876,580 bp
  • A to G, chromosome 2 at 180,172,937 bp
  • C to T, chromosome 3 at 27,542,104 bp
  • T to C, chromosome 3 at 55,128,558 bp
  • T to C, chromosome 3 at 76,707,831 bp
  • T to A, chromosome 3 at 99,314,903 bp
  • T to C, chromosome 3 at 130,009,858 bp
  • T to C, chromosome 3 at 138,468,882 bp
  • T to C, chromosome 3 at 155,188,182 bp
  • T to C, chromosome 4 at 96,658,740 bp
  • G to T, chromosome 5 at 16,593,990 bp
  • T to C, chromosome 5 at 96,769,507 bp
  • T to C, chromosome 5 at 110,297,788 bp
  • T to C, chromosome 5 at 145,132,862 bp
  • T to C, chromosome 5 at 150,719,914 bp
  • C to T, chromosome 6 at 122,609,797 bp
  • G to T, chromosome 6 at 123,069,769 bp
  • A to T, chromosome 7 at 29,309,749 bp
  • A to T, chromosome 7 at 45,830,632 bp
  • T to C, chromosome 7 at 75,609,004 bp
  • C to T, chromosome 7 at 90,375,602 bp
  • C to T, chromosome 7 at 109,716,026 bp
  • C to T, chromosome 7 at 122,170,140 bp
  • C to G, chromosome 7 at 126,493,756 bp
  • T to G, chromosome 7 at 126,979,653 bp
  • T to C, chromosome 8 at 71,651,191 bp
  • ACTCGAAATGATGTGGCTC to ACTC, chromosome 8 at 106,666,238 bp
  • A to G, chromosome 9 at 8,643,410 bp
  • A to G, chromosome 9 at 78,367,883 bp
  • T to A, chromosome 9 at 111,378,935 bp
  • C to A, chromosome 9 at 118,049,976 bp
  • T to G, chromosome 9 at 122,853,667 bp
  • T to A, chromosome 10 at 18,603,455 bp
  • A to G, chromosome 10 at 76,170,783 bp
  • A to G, chromosome 10 at 79,396,707 bp
  • A to G, chromosome 10 at 97,675,745 bp
  • A to G, chromosome 11 at 55,137,311 bp
  • T to C, chromosome 11 at 95,306,156 bp
  • A to T, chromosome 12 at 98,689,137 bp
  • A to T, chromosome 12 at 116,452,363 bp
  • T to A, chromosome 13 at 22,883,324 bp
  • A to G, chromosome 13 at 40,728,832 bp
  • A to G, chromosome 13 at 59,800,128 bp
  • A to T, chromosome 14 at 103,685,728 bp
  • C to A, chromosome 15 at 10,393,744 bp
  • T to A, chromosome 16 at 15,630,425 bp
  • G to A, chromosome 16 at 31,456,894 bp
  • T to G, chromosome 16 at 32,753,994 bp
  • A to T, chromosome 16 at 32,754,017 bp
  • C to A, chromosome 16 at 35,647,367 bp
  • C to A, chromosome 17 at 13,015,087 bp
  • T to C, chromosome 17 at 34,030,990 bp
  • A to T, chromosome 17 at 34,917,532 bp
  • T to C, chromosome 17 at 71,831,208 bp
  • A to G, chromosome 18 at 20,688,365 bp
  • G to T, chromosome 18 at 64,511,824 bp
  • A to G, chromosome 18 at 84,675,173 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8929 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068773-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.