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Strain Name:
C57BL/6J-MtgxR8969Btlr/Mmmh
Stock Number:
068803-MU
Citation ID:
RRID:MMRRC_068803-MU
Other Names:
R8969 (G1)
Major Collection:

Strain Information

Kit
Name: KIT proto-oncogene receptor tyrosine kinase
Synonyms: Steel Factor Receptor, c-KIT, Dominant white spotting, belly-spot, Tr-kit, SOW3, SCO5, SCO1, Gsfsow3, Gsfsco5, Gsfsco1, CD117
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16590
HGNC: HGNC:6342
Homologene: 187
Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: E330008K23Rik, HALR, Mll3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231051
Homologene: 46480
Dspp
Name: dentin sialophosphoprotein
Synonyms: Dmp3, Dpp, Dsp
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 666279
HGNC: HGNC:3054
Chrnb2
Name: cholinergic receptor nicotinic beta 2 subunit
Synonyms: [b]2-nAchR, Acrb-2, Acrb2, C030030P04Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11444
HGNC: HGNC:1962
Homologene: 595
Crkl
Name: v-crk avian sarcoma virus CT10 oncogene homolog-like
Synonyms: Crkol, 1110025F07Rik, snoop
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12929
HGNC: HGNC:2363
Homologene: 38021
Aldh1l1
Name: aldehyde dehydrogenase 1 family, member L1
Synonyms: 1810048F20Rik, Fthfd
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 107747
HGNC: HGNC:3978
Homologene: 122031
Srd5a3
Name: steroid 5 alpha-reductase 3
Synonyms: D730040M03Rik, 1110025P14Rik, Srd5a2l
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 57357
Homologene: 41385
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 107,524,660 bp
  • A to T, chromosome 1 at 127,960,851 bp
  • G to T, chromosome 2 at 29,346,863 bp
  • A to G, chromosome 2 at 35,019,463 bp
  • A to G, chromosome 2 at 37,428,665 bp
  • A to G, chromosome 2 at 85,869,488 bp
  • T to A, chromosome 2 at 87,150,584 bp
  • A to T, chromosome 2 at 90,902,429 bp
  • C to T, chromosome 2 at 131,053,074 bp
  • T to C, chromosome 2 at 147,029,384 bp
  • A to G, chromosome 2 at 181,237,788 bp
  • T to A, chromosome 3 at 27,222,736 bp
  • A to G, chromosome 3 at 30,818,875 bp
  • T to C, chromosome 3 at 87,725,642 bp
  • G to A, chromosome 3 at 87,929,975 bp
  • T to C, chromosome 3 at 88,966,291 bp
  • A to G, chromosome 3 at 89,757,225 bp
  • A to T, chromosome 4 at 34,692,522 bp
  • A to T, chromosome 4 at 44,149,079 bp
  • C to T, chromosome 4 at 63,154,091 bp
  • T to A, chromosome 5 at 14,522,194 bp
  • C to T, chromosome 5 at 24,387,834 bp
  • T to A, chromosome 5 at 25,314,389 bp
  • A to C, chromosome 5 at 72,559,130 bp
  • G to A, chromosome 5 at 75,639,062 bp
  • A to G, chromosome 5 at 76,153,646 bp
  • T to A, chromosome 5 at 86,713,899 bp
  • A to T, chromosome 5 at 104,177,774 bp
  • A to T, chromosome 5 at 146,112,820 bp
  • T to C, chromosome 6 at 71,903,447 bp
  • C to T, chromosome 6 at 86,651,484 bp
  • A to G, chromosome 6 at 90,570,808 bp
  • G to A, chromosome 7 at 28,607,957 bp
  • C to A, chromosome 7 at 39,409,308 bp
  • T to A, chromosome 7 at 44,054,508 bp
  • A to G, chromosome 7 at 45,679,473 bp
  • T to C, chromosome 7 at 102,714,351 bp
  • T to G, chromosome 7 at 103,098,044 bp
  • A to T, chromosome 7 at 113,325,955 bp
  • T to A, chromosome 7 at 143,638,436 bp
  • A to C, chromosome 8 at 61,384,826 bp
  • T to A, chromosome 8 at 61,684,849 bp
  • A to G, chromosome 8 at 112,011,738 bp
  • T to C, chromosome 8 at 119,870,148 bp
  • T to C, chromosome 9 at 7,130,723 bp
  • T to C, chromosome 9 at 22,225,834 bp
  • T to C, chromosome 9 at 82,926,964 bp
  • A to G, chromosome 10 at 79,478,019 bp
  • T to C, chromosome 10 at 128,671,673 bp
  • T to A, chromosome 11 at 9,277,944 bp
  • C to T, chromosome 11 at 36,051,861 bp
  • T to A, chromosome 11 at 70,660,865 bp
  • T to A, chromosome 11 at 73,711,873 bp
  • T to C, chromosome 11 at 91,577,193 bp
  • T to A, chromosome 11 at 99,387,608 bp
  • T to A, chromosome 11 at 100,102,303 bp
  • C to T, chromosome 11 at 100,379,565 bp
  • G to T, chromosome 12 at 75,453,718 bp
  • T to A, chromosome 12 at 98,689,025 bp
  • A to G, chromosome 13 at 24,422,653 bp
  • T to A, chromosome 13 at 45,391,344 bp
  • A to G, chromosome 13 at 55,085,634 bp
  • A to T, chromosome 13 at 98,755,096 bp
  • A to G, chromosome 13 at 116,303,321 bp
  • T to C, chromosome 14 at 122,941,812 bp
  • A to G, chromosome 15 at 21,492,653 bp
  • T to G, chromosome 15 at 102,490,779 bp
  • A to G, chromosome 16 at 17,469,054 bp
  • A to T, chromosome 16 at 45,772,133 bp
  • T to G, chromosome 16 at 78,547,794 bp
  • T to C, chromosome 16 at 91,404,172 bp
  • C to T, chromosome 17 at 5,725,280 bp
  • T to A, chromosome 17 at 26,087,381 bp
  • T to C, chromosome 17 at 29,705,579 bp
  • C to T, chromosome 17 at 31,648,880 bp
  • T to C, chromosome 17 at 35,055,410 bp
  • T to A, chromosome 17 at 53,660,088 bp
  • G to A, chromosome 19 at 20,816,229 bp
  • A to G, chromosome 19 at 22,925,944 bp
  • T to G, chromosome 19 at 29,047,694 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8969 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068803-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.