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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR8987Btlr/Mmmh
Stock Number:
068819-MU
Citation ID:
RRID:MMRRC_068819-MU
Other Names:
R8987 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Cntnap2
Name: contactin associated protein-like 2
Synonyms: 5430425M22Rik, Caspr2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 66797
Homologene: 69159

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D630045J12Rik
Name: RIKEN cDNA D630045J12 gene
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330286
Homologene: 19782

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Satb1
Name: special AT-rich sequence binding protein 1
Synonyms: 2610306G12Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20230
Homologene: 2232

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Actn4
Name: actinin alpha 4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 60595
HGNC: HGNC:166
Homologene: 55857

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Chtf8
Name: CTF8, chromosome transmission fidelity factor 8
Synonyms: 5830457O10Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 214987
Homologene: 84588

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IMPC
Google
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Hspa12a
Name: heat shock protein 12A
Synonyms: Hspa12a, 1700063D12Rik, Gm19925
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 73442
VEGA: 19
Homologene: 18422

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Tacr3
Name: tachykinin receptor 3
Synonyms: neuromedin K receptor, Tac3r, Nk3r
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 21338
Homologene: 824

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 45,911,335 bp
  • G to A, chromosome 1 at 54,661,190 bp
  • A to G, chromosome 1 at 93,778,359 bp
  • G to A, chromosome 2 at 120,269,961 bp
  • A to C, chromosome 2 at 130,038,483 bp
  • C to T, chromosome 2 at 142,849,863 bp
  • T to C, chromosome 2 at 142,901,358 bp
  • A to G, chromosome 2 at 163,858,297 bp
  • T to C, chromosome 2 at 178,067,798 bp
  • G to A, chromosome 2 at 178,067,799 bp
  • T to C, chromosome 3 at 51,690,447 bp
  • T to C, chromosome 3 at 54,194,711 bp
  • C to T, chromosome 3 at 87,085,093 bp
  • C to T, chromosome 3 at 87,730,481 bp
  • T to C, chromosome 3 at 88,360,407 bp
  • T to A, chromosome 3 at 94,940,114 bp
  • T to A, chromosome 3 at 134,854,812 bp
  • T to G, chromosome 3 at 134,854,957 bp
  • G to A, chromosome 3 at 154,709,703 bp
  • G to A, chromosome 4 at 16,123,699 bp
  • G to A, chromosome 4 at 44,645,661 bp
  • T to A, chromosome 4 at 152,103,915 bp
  • G to T, chromosome 4 at 155,660,894 bp
  • T to C, chromosome 5 at 8,927,931 bp
  • A to T, chromosome 5 at 30,364,095 bp
  • G to T, chromosome 5 at 33,668,825 bp
  • T to G, chromosome 5 at 34,820,024 bp
  • A to T, chromosome 5 at 38,299,727 bp
  • T to C, chromosome 5 at 112,337,055 bp
  • T to C, chromosome 5 at 113,692,752 bp
  • C to A, chromosome 5 at 119,680,821 bp
  • T to A, chromosome 5 at 120,867,089 bp
  • C to T, chromosome 5 at 137,911,587 bp
  • A to T, chromosome 5 at 145,374,229 bp
  • G to T, chromosome 5 at 145,374,230 bp
  • G to A, chromosome 6 at 8,950,312 bp
  • A to T, chromosome 6 at 34,160,507 bp
  • A to G, chromosome 6 at 38,196,963 bp
  • A to T, chromosome 6 at 40,729,636 bp
  • G to T, chromosome 6 at 46,484,049 bp
  • A to T, chromosome 6 at 48,835,596 bp
  • A to T, chromosome 6 at 89,661,495 bp
  • T to A, chromosome 6 at 133,853,726 bp
  • T to A, chromosome 6 at 142,356,525 bp
  • C to T, chromosome 7 at 4,776,030 bp
  • A to G, chromosome 7 at 28,896,973 bp
  • G to T, chromosome 7 at 30,877,747 bp
  • A to T, chromosome 7 at 46,287,454 bp
  • A to T, chromosome 7 at 50,848,651 bp
  • A to T, chromosome 7 at 65,927,227 bp
  • T to A, chromosome 7 at 74,320,576 bp
  • C to A, chromosome 7 at 81,560,018 bp
  • T to A, chromosome 8 at 13,470,294 bp
  • T to A, chromosome 8 at 43,569,321 bp
  • T to A, chromosome 8 at 106,886,103 bp
  • T to C, chromosome 8 at 109,732,485 bp
  • T to A, chromosome 8 at 110,513,134 bp
  • C to T, chromosome 8 at 123,297,799 bp
  • A to T, chromosome 9 at 18,551,685 bp
  • T to C, chromosome 9 at 20,476,448 bp
  • T to A, chromosome 9 at 36,120,990 bp
  • A to T, chromosome 9 at 39,772,392 bp
  • T to C, chromosome 9 at 50,895,347 bp
  • A to G, chromosome 9 at 66,250,320 bp
  • A to T, chromosome 9 at 76,130,310 bp
  • A to G, chromosome 9 at 83,401,743 bp
  • C to T, chromosome 9 at 108,294,509 bp
  • T to A, chromosome 9 at 109,108,110 bp
  • T to A, chromosome 9 at 111,271,968 bp
  • T to C, chromosome 9 at 123,829,074 bp
  • A to G, chromosome 10 at 5,227,579 bp
  • A to G, chromosome 10 at 20,963,784 bp
  • A to G, chromosome 10 at 23,900,816 bp
  • C to T, chromosome 10 at 79,293,870 bp
  • C to A, chromosome 10 at 127,182,866 bp
  • A to G, chromosome 11 at 22,053,531 bp
  • A to T, chromosome 11 at 23,464,267 bp
  • T to C, chromosome 11 at 50,266,748 bp
  • A to T, chromosome 11 at 72,112,306 bp
  • G to A, chromosome 11 at 83,069,537 bp
  • A to G, chromosome 11 at 87,763,568 bp
  • A to T, chromosome 11 at 94,670,452 bp
  • C to T, chromosome 11 at 97,775,684 bp
  • A to T, chromosome 11 at 98,892,266 bp
  • A to G, chromosome 11 at 100,103,546 bp
  • A to C, chromosome 12 at 86,161,501 bp
  • C to T, chromosome 12 at 113,491,128 bp
  • A to T, chromosome 13 at 54,467,895 bp
  • C to T, chromosome 13 at 58,571,440 bp
  • A to T, chromosome 13 at 93,452,889 bp
  • G to T, chromosome 13 at 98,053,964 bp
  • A to G, chromosome 13 at 100,067,513 bp
  • A to G, chromosome 13 at 100,426,926 bp
  • C to T, chromosome 14 at 31,311,747 bp
  • C to T, chromosome 14 at 49,015,529 bp
  • T to C, chromosome 14 at 56,526,926 bp
  • T to A, chromosome 14 at 60,756,447 bp
  • C to T, chromosome 14 at 66,807,953 bp
  • A to T, chromosome 14 at 103,208,796 bp
  • T to C, chromosome 15 at 4,814,862 bp
  • T to A, chromosome 15 at 27,732,687 bp
  • G to A, chromosome 15 at 64,817,907 bp
  • T to A, chromosome 15 at 71,462,340 bp
  • A to G, chromosome 15 at 85,868,908 bp
  • A to T, chromosome 15 at 96,418,676 bp
  • A to T, chromosome 15 at 98,791,743 bp
  • A to C, chromosome 16 at 17,804,904 bp
  • A to T, chromosome 16 at 17,925,880 bp
  • A to G, chromosome 16 at 18,811,550 bp
  • A to G, chromosome 16 at 43,931,347 bp
  • C to T, chromosome 16 at 88,562,845 bp
  • A to T, chromosome 17 at 25,105,428 bp
  • T to A, chromosome 17 at 25,309,421 bp
  • A to C, chromosome 17 at 32,798,827 bp
  • A to G, chromosome 17 at 37,169,976 bp
  • A to T, chromosome 17 at 37,170,099 bp
  • A to T, chromosome 17 at 51,805,353 bp
  • A to T, chromosome 17 at 81,647,853 bp
  • T to G, chromosome 18 at 20,682,924 bp
  • T to A, chromosome 18 at 32,093,592 bp
  • A to G, chromosome 18 at 34,690,826 bp
  • T to C, chromosome 18 at 34,947,929 bp
  • T to A, chromosome 18 at 36,999,945 bp
  • A to T, chromosome 18 at 39,357,599 bp
  • A to G, chromosome 18 at 49,893,852 bp
  • A to T, chromosome 19 at 4,004,511 bp
  • A to G, chromosome 19 at 22,918,760 bp
  • G to T, chromosome 19 at 55,083,795 bp
  • T to A, chromosome 19 at 58,799,471 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8987 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
068819-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.