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Strain Name:
C57BL/6J-MtgxR8998Btlr/Mmmh
Stock Number:
068829-MU
Citation ID:
RRID:MMRRC_068829-MU
Other Names:
R8998 (G1)
Major Collection:

Strain Information

Tyrp1
Name: tyrosinase-related protein 1
Synonyms: Tyrp, isa, TRP-1, TRP1, Oca3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 22178
Homologene: 464
Met
Name: met proto-oncogene
Synonyms: HGF receptor, c-Met, Par4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17295
HGNC: HGNC:7029
Homologene: 206
Gabrg1
Name: gamma-aminobutyric acid type A receptor subunit gamma 1
Synonyms: GabaA
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14405
HGNC: HGNC:4086
Homologene: 22570
Ptprf
Name: protein tyrosine phosphatase receptor type F
Synonyms: LAR, RPTP-LAR
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 19268
HGNC: HGNC:9670
Homologene: 20623
Mfn1
Name: mitofusin 1
Synonyms: 6330416C07Rik, 2310002F04Rik, D3Ertd265e, HR2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 67414
Homologene: 11481
Slc4a7
Name: solute carrier family 4, sodium bicarbonate cotransporter, member 7
Synonyms: E430014N10Rik, NBC3, NBCn1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 218756
VEGA: 14
Homologene: 2680
Plekha1
Name: pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1
Synonyms: TAPP1, C920009D07Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101476
Homologene: 11001
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 20,364,201 bp
  • A to T, chromosome 1 at 66,413,314 bp
  • T to C, chromosome 1 at 69,896,547 bp
  • C to A, chromosome 1 at 89,929,023 bp
  • A to G, chromosome 1 at 138,101,192 bp
  • C to T, chromosome 1 at 181,818,947 bp
  • T to C, chromosome 2 at 59,969,264 bp
  • T to C, chromosome 2 at 62,537,024 bp
  • T to G, chromosome 2 at 70,531,620 bp
  • T to C, chromosome 2 at 76,827,933 bp
  • T to A, chromosome 2 at 80,630,927 bp
  • T to A, chromosome 2 at 87,902,845 bp
  • C to T, chromosome 2 at 90,139,128 bp
  • C to A, chromosome 2 at 129,810,589 bp
  • A to G, chromosome 2 at 165,381,488 bp
  • A to G, chromosome 3 at 28,665,771 bp
  • T to A, chromosome 3 at 32,569,534 bp
  • A to C, chromosome 3 at 46,446,348 bp
  • A to C, chromosome 3 at 69,027,561 bp
  • T to C, chromosome 3 at 92,418,869 bp
  • A to G, chromosome 3 at 100,931,644 bp
  • A to T, chromosome 3 at 106,821,683 bp
  • T to C, chromosome 4 at 62,324,128 bp
  • G to C, chromosome 4 at 73,919,183 bp
  • T to C, chromosome 4 at 80,844,857 bp
  • C to A, chromosome 4 at 81,284,645 bp
  • A to G, chromosome 4 at 107,080,960 bp
  • A to G, chromosome 4 at 118,226,474 bp
  • A to G, chromosome 4 at 129,622,678 bp
  • A to G, chromosome 5 at 14,677,496 bp
  • T to A, chromosome 5 at 70,816,035 bp
  • G to A, chromosome 5 at 100,692,243 bp
  • T to C, chromosome 5 at 101,664,216 bp
  • A to C, chromosome 5 at 101,845,192 bp
  • C to T, chromosome 6 at 17,491,535 bp
  • T to C, chromosome 6 at 30,666,166 bp
  • C to A, chromosome 6 at 70,193,530 bp
  • A to C, chromosome 6 at 119,242,915 bp
  • A to G, chromosome 6 at 120,294,929 bp
  • T to C, chromosome 7 at 3,842,491 bp
  • A to G, chromosome 7 at 10,715,702 bp
  • A to G, chromosome 7 at 28,338,423 bp
  • A to C, chromosome 7 at 85,411,099 bp
  • C to A, chromosome 7 at 98,619,305 bp
  • T to C, chromosome 7 at 104,768,762 bp
  • A to C, chromosome 7 at 108,845,810 bp
  • T to C, chromosome 7 at 130,908,469 bp
  • T to A, chromosome 7 at 142,202,196 bp
  • T to C, chromosome 8 at 48,276,687 bp
  • A to G, chromosome 8 at 88,250,722 bp
  • A to T, chromosome 9 at 26,853,618 bp
  • T to C, chromosome 9 at 38,473,491 bp
  • A to G, chromosome 9 at 44,821,877 bp
  • G to A, chromosome 9 at 49,037,839 bp
  • T to C, chromosome 9 at 53,229,234 bp
  • A to T, chromosome 10 at 63,162,271 bp
  • A to G, chromosome 10 at 129,473,517 bp
  • A to G, chromosome 11 at 11,685,013 bp
  • C to T, chromosome 11 at 30,838,957 bp
  • T to A, chromosome 11 at 43,259,899 bp
  • T to C, chromosome 11 at 60,949,991 bp
  • T to C, chromosome 11 at 67,927,311 bp
  • A to G, chromosome 11 at 69,142,225 bp
  • T to C, chromosome 11 at 72,030,257 bp
  • A to T, chromosome 11 at 104,749,651 bp
  • G to A, chromosome 11 at 120,654,381 bp
  • T to C, chromosome 12 at 73,696,199 bp
  • C to G, chromosome 12 at 103,651,729 bp
  • A to G, chromosome 13 at 21,347,818 bp
  • T to A, chromosome 14 at 14,775,346 bp
  • T to C, chromosome 14 at 31,296,278 bp
  • C to T, chromosome 14 at 33,962,403 bp
  • T to A, chromosome 14 at 56,130,687 bp
  • T to C, chromosome 14 at 75,583,214 bp
  • T to A, chromosome 15 at 76,102,736 bp
  • A to C, chromosome 15 at 102,031,439 bp
  • A to G, chromosome 16 at 22,953,705 bp
  • G to C, chromosome 17 at 14,950,621 bp
  • G to A, chromosome 17 at 25,963,081 bp
  • A to G, chromosome 17 at 48,302,747 bp
  • A to T, chromosome 17 at 56,563,391 bp
  • T to A, chromosome 17 at 85,623,736 bp
  • C to A, chromosome 18 at 7,211,574 bp
  • T to C, chromosome 18 at 20,533,627 bp
  • C to T, chromosome 18 at 36,940,375 bp
  • C to T, chromosome 18 at 42,737,928 bp
  • T to C, chromosome 18 at 44,864,304 bp
  • G to A, chromosome 19 at 6,339,930 bp
  • A to T, chromosome 19 at 10,799,278 bp
  • G to A, chromosome 19 at 34,936,853 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8998 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068829-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.